NB4Homo sapiens (Human)Cancer cell line

Also known as: NB.4, NB-4

🤖 AI SummaryBased on 12 publications

Quick Overview

NB4 is a human acute promyelocytic leukemia cell line used in research for APL and leukemia studies.

Detailed Summary

NB4 is a well-characterized human acute promyelocytic leukemia (APL) cell line derived from a patient with t(15;17) chromosomal translocation. It is widely used in research for studying APL pathogenesis, drug responses, and differentiation mechanisms. NB4 cells are sensitive to all-trans-retinoic acid (ATRA) and can be used to investigate the effects of various therapies, including arsenic trioxide and tyrosine kinase inhibitors. The cell line is also utilized in studies of genetic mutations, such as those involving the JAK2 V617F mutation and cohesin complex alterations. NB4 has been instrumental in understanding the molecular mechanisms of APL and has contributed to the development of targeted therapies for leukemia.

Research Applications

Acute Promyelocytic Leukemia (APL) researchDrug response studiesDifferentiation mechanismsGenetic mutation analysisTargeted therapy development

Key Characteristics

t(15;17) chromosomal translocationATRA sensitivityArsenic trioxide responsivenessJAK2 V617F mutation studiesCohesin complex alterations

Generated on 6/14/2025

Basic Information

Database ID	CVCL_0005
Species	Homo sapiens (Human)
Tissue Source	Bone marrow[UBERON:UBERON_0002371]

Donor Information

Age	23
Age Category	Adult
Sex	Female

Disease Information

Disease	Acute promyelocytic leukemia
Lineage	Myeloid
Subtype	Acute Myeloid Leukemia
OncoTree Code	AML

DepMap Information

Source Type	DSMZ
Source ID	ACH-000294_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg248Gln (c.743G>A)	Unspecified	Somatic mutation acquired during proliferation	PubMed=20575032
MutationSimple	KRAS	p.Ala18Asp (c.53C>A)	Heterozygous	-	from parent cell line NB4
Gene fusion	PML	PML-RARA	-	-	PubMed=8781440

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,12

D13S317

11,12

D16S539

D18S51

12,14

D19S433

D21S11

28,33.2

D2S1338

20,25

D3S1358

15,17

D5S818

D7S820

10,13

D8S1179

10,14

FGA

21,22

Penta D

10,13

Penta E

7,13

TH01

7,9.3

TPOX

8,11

vWA

16,19

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

The LL-100 panel: 100 cell lines for blood cancer studies.";

MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.

Sci. Rep. 9:8218-8218(2019).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

DOI PubMed PMC

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOI PubMed PMC

A mass spectrometric-derived cell surface protein atlas.";

Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.

PLoS ONE 10:E0121314-E0121314(2015).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.

Haferlach T., Shirahige K., Miyano S., Ogawa S.

Nat. Genet. 45:1232-1237(2013).

DOI PubMed

Dynamic DNA methylation across diverse human cell lines and tissues.

Crawford G.E., Absher D.M., Wold B.J., Myers R.M.

Genome Res. 23:555-567(2013).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

Leukemia 20:471-476(2006).

DOI PubMed

Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.

Fioretos T.

Leukemia 19:1042-1050(2005).

DOI PubMed

FLT3 mutations in the activation loop of tyrosine kinase domain are frequently found in infant ALL with MLL rearrangements and pediatric ALL with hyperdiploidy.

Tsuchida M., Sugita K., Ida K., Hayashi Y.

Blood 103:1085-1088(2004).

DOI PubMed

Molecular cytogenetics of the acute promyelocytic leukemia-derived cell line NB4 and of four all-trans retinoic acid-resistant subclones.

Miller W.H. Jr., Lafage-Pochitaloff M.

Genes Chromosomes Cancer 35:261-270(2002).

DOI PubMed

Maturation sensitive and resistant t(15;17) NB4 cell lines as tools for APL physiopathology: nomenclature of cells and repertory of their known genetic alterations and phenotypes.

Roussel M.J.S., Lanotte M.

Oncogene 20:7287-7291(2001).

DOI PubMed

The induction of apoptosis and cell cycle arrest by arsenic trioxide in lymphoid neoplasms.

Takeshita K., Takeshita A., Ohno R.

Leukemia 12:1383-1391(1998).

DOI PubMed

Distinct apoptotic responses in maturation sensitive and resistant t(15;17) acute promyelocytic leukemia NB4 cells. 9-cis retinoic acid induces apoptosis independent of maturation and Bcl-2 expression.

Bruel A., Benoit G., De Nay D., Brown S., Lanotte M.

Leukemia 9:1173-1184(1995).

PubMed

NB4, a maturation inducible cell line with t(15;17) marker isolated from a human acute promyelocytic leukemia (M3).

Berger R.

Blood 77:1080-1086(1991).

DOI PubMed

A retinoid acid 'resistant' t(15;17) acute promyelocytic leukemia cell line: isolation, morphological, immunological, and molecular features.

Kastner P., Berger R., Lanotte M.

Leukemia 6:1281-1287(1992).

PubMed

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI

Web Resources

proteinatlas.org genome.ucsc.edu