THP-1Homo sapiens (Human)Cancer cell line
Also known as: THP1, THP 1, THPI, THP-1(ATCC), THP-1-O, O-THP-1, Tohoku Hospital Pediatrics-1
Quick Overview
THP-1 is a human monocytic cell line used in cancer and immunology research.
Detailed Summary
Research Applications
Key Characteristics
Basic Information
| Database ID | CVCL_0006 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Peripheral blood[UBERON:UBERON_0000178] |
Donor Information
| Age | 1 |
|---|---|
| Age Category | Pediatric |
| Sex | Male |
Disease Information
| Disease | Childhood acute monocytic leukemia |
|---|---|
| Lineage | Myeloid |
| Subtype | Acute Myeloid Leukemia |
| OncoTree Code | AML |
DepMap Information
| Source Type | ATCC |
|---|---|
| Source ID | ACH-000146_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| Gene fusion | CSNK2A1 | CSNK2A1-DDX39B | - | - | from parent cell line THP-1 |
| Gene fusion | KMT2A | KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 | - | - | PubMed=31701557 |
| MutationSimple | NRAS | p.Gly12Asp (c.35G>A) | Unspecified | Somatic | Wistar |
| MutationSimple | TP53 | p.Arg174fs*3 (c.520_545del26) | Heterozygous | - | from parent cell line THP-1 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Loading gene expression data...
Publications
Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.
Chauhan V., Howland M.
ScientificWorldJournal 2012:205038.1-205038.9(2012).
Pan-cancer proteomic map of 949 human cell lines.";
Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Cancer Cell 40:835-849.e8(2022).
THP-1 reference data: proposal of an in vitro branched evolution model for cancer cell lines.
Kasai F., Hirayama N., Fukushima M., Kohara A., Nakamura Y.
Int. J. Cancer 151:463-472(2022).
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021).
In-depth mining of the immunopeptidome of an acute myeloid leukemia cell line using complementary ligand enrichment and data acquisition strategies.
Ramarathinam S.H., Purcell A.W.
Mol. Immunol. 123:7-17(2020).
Quantitative proteomics of the Cancer Cell Line Encyclopedia.";
Sellers W.R., Gygi S.P.
Cell 180:387-402.e16(2020).
The LL-100 panel: 100 cell lines for blood cancer studies.";
MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.
Sci. Rep. 9:8218-8218(2019).
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Sellers W.R.
Nature 569:503-508(2019).
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
Cancer Res. 79:1263-1273(2019).
Screening human cell lines for viral infections applying RNA-Seq data analysis.
Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.
PLoS ONE 14:E0210404-E0210404(2019).
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
Yang H.H., Koeffler H.P.
BMC Cancer 18:940.1-940.13(2018).
Adaptation of the human Cell Line Activation Test (h-CLAT) to animal-product-free conditions.
Edwards A., Roscoe L., Longmore C., Bailey F., Sim B., Treasure C.
ALTEX 35:477-488(2018).
THP-1 and U937 cells.";
Chanput W., Peters V., Wichers H.J.
(In book chapter) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx K., Cotter P., Lopez-Exposito I., Kleiveland C., Lea T., Mackie A., Requena T., Swiatecka D., Wichers H. (eds.); pp.147-159; Springer; Cham; Switzerland (2015).
Co-culture of human alveolar epithelial (hAELVi) and macrophage (THP-1) cell lines.
Schneider-Daum N., de Souza Carvalho-Wodarz C., Lehr C.-M.
ALTEX 35:211-222(2018).
Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFalpha expression.
Moore C., Kuhn A., Behnert A., Guthke R., Driesch D., Slevogt H.
Sci. Rep. 7:838-838(2017).
Characterization of human cancer cell lines by reverse-phase protein arrays.
Liang H.
Cancer Cell 31:225-239(2017).
Hh/Gli antagonist in acute myeloid leukemia with CBFA2T3-GLIS2 fusion gene.
Pigazzi M., Martelli A.M., Basso G., Locatelli F., Pession A.
J. Hematol. Oncol. 10:26.1-26.5(2017).
A landscape of pharmacogenomic interactions in cancer.";
Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
Cell 166:740-754(2016).
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Loewer M., Sahin U., Castle J.C.
Genome Med. 7:118.1-118.7(2015).
Multicomponent coculture system of cancer cells and two types of stromal cells for in vitro evaluation of anticancer drugs.
Yamazoe H., Hagihara Y., Kobayashi H.
Tissue Eng. Part C. Methods 22:20-29(2016).
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Golub T.R., Root D.E., Hahn W.C.
Sci. Data 1:140035-140035(2014).
A resource for cell line authentication, annotation and quality control.
Neve R.M.
Nature 520:307-311(2015).
A comprehensive transcriptional portrait of human cancer cell lines.
Settleman J., Seshagiri S., Zhang Z.-M.
Nat. Biotechnol. 33:306-312(2015).
THP-1 cell line: an in vitro cell model for immune modulation approach.
Chanput W., Mes J.J., Wichers H.J.
Int. Immunopharmacol. 23:37-45(2014).
Mechanisms of resistance to azacitidine in human leukemia cell lines.
Ohmine K., Muroi K., Ozawa K.
Exp. Hematol. 42:294-306.e2(2014).
Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.
Haferlach T., Shirahige K., Miyano S., Ogawa S.
Nat. Genet. 45:1232-1237(2013).
The leukemia-lymphoma cell line factsbook.";
Drexler H.G.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).
Frequent mutations in the p53 gene in human myeloid leukemia cell lines.
Ishikawa F., Takaku F., Yazaki Y., Hirai H.
Blood 79:2378-2383(1992).
Inhibition of phorbol ester-induced cell activation in microgravity.
Limouse M., Manie S., Konstantinova I., Ferrua B., Schaffar L.
Exp. Cell Res. 197:82-86(1991).
Characterization of resistance to VP-16 in human leukemic cell line.
Saijo Y., Kumano N., Tokue Y., Satoh K., Oizumi K., Motomiya M.
Tohoku J. Exp. Med. 159:299-306(1989).
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1. carboxylic esterase.
Drexler H.G., Gaedicke G., Minowada J.
Leuk. Res. 9:209-229(1985).
Isoenzyme studies in human leukemia-lymphoma cell lines -- III. Beta-hexosaminidase (E.C. 3.2.1.30).
Drexler H.G., Gaedicke G., Minowada J.
Leuk. Res. 9:549-559(1985).
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Drexler H.G., Gaedicke G., Minowada J.
Leuk. Res. 9:537-548(1985).
Lineage infidelity of a human myelogenous leukemia cell line.";
Palumbo A., Minowada J., Erikson J., Croce C.M., Rovera G.
Blood 64:1059-1063(1984).
Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester.
Tada K.
Cancer Res. 42:1530-1536(1982).
Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).
Tsuchiya S., Yamabe M., Yamaguchi Y., Kobayashi Y., Konno T., Tada K.
Int. J. Cancer 26:171-176(1980).
Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.
Imaizumi M., Endo M., Takano N., Konno T.
Leukemia 10:102-105(1996).
Screening the p53 status of human cell lines using a yeast functional assay.
Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Mol. Carcinog. 19:243-253(1997).
Mutations of the RAS genes in childhood acute myeloid leukemia, myelodysplastic syndrome and juvenile chronic myelocytic leukemia.
Kobayashi M., Bessho F., Yanagisawa M., Hayashi Y.
Leuk. Res. 21:697-701(1997).
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Hirai H.
Jpn. J. Cancer Res. 89:33-39(1998).
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Aizawa Y., Ueda R., Seto M.
Jpn. J. Cancer Res. 89:712-718(1998).
Establishment of an activated macrophage cell line, A-THP-1, and its properties.
Tominaga T., Suzuki M., Saeki H., Matsuno S., Tachibana T., Kudo T.
Tohoku J. Exp. Med. 186:99-119(1998).
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Leuk. Res. 24:255-262(2000).
Cytogenetic and molecular analysis of the acute monocytic leukemia cell line THP-1 with an MLL-AF9 translocation.
Odero M.D., Zeleznik-Le N.J., Chinwalla V., Rowley J.D.
Genes Chromosomes Cancer 29:333-338(2000).
Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Leuk. Res. 25:275-278(2001).
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
Wolf E., Gabius H.-J.
J. Cancer Res. Clin. Oncol. 127:375-386(2001).
Short tandem repeat profiling provides an international reference standard for human cell lines.
Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).
Mutations of the BRAF gene in human cancer.";
Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Nature 417:949-954(2002).
FLT3 mutations in the activation loop of tyrosine kinase domain are frequently found in infant ALL with MLL rearrangements and pediatric ALL with hyperdiploidy.
Tsuchida M., Sugita K., Ida K., Hayashi Y.
Blood 103:1085-1088(2004).
Malignant hematopoietic cell lines: in vitro models for the study of MLL gene alterations.
Drexler H.G., Quentmeier H., MacLeod R.A.F.
Leukemia 18:227-232(2004).
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Fioretos T.
Leukemia 19:1042-1050(2005).
Development of an in vitro skin sensitization test using human cell lines: the human Cell Line Activation Test (h-CLAT). I. Optimization of the h-CLAT protocol.
Sakaguchi H., Miyazawa M., Ito Y., Suzuki H., Toyoda H.
Toxicol. In Vitro 20:767-773(2006).
Development of an in vitro skin sensitization test using human cell lines; human Cell Line Activation Test (h-CLAT). II. An inter-laboratory study of the h-CLAT.
Yoneyama K., Hirota M., Itagaki H., Toyoda H., Suzuki H.
Toxicol. In Vitro 20:774-784(2006).
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.
Leukemia 20:471-476(2006).
Aberrant p53 protein expression and function in a panel of hematopoietic cell lines with different p53 mutations.
Hasegawa H., Yamada Y.
Eur. J. Haematol. 82:301-307(2009).
The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line.
Tagami M., Takahashi Y., Kawai J., Hayashizaki Y.
Nat. Genet. 41:553-562(2009).
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Haber D.A.
Cancer Res. 70:2158-2164(2010).
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
Nature 483:603-607(2012).
Effects of alpha-particle radiation on microRNA responses in human cell-lines.
Chauhan V., Howland M., Wilkins R.C.
Open Biochem. J. 6:16-22(2012).
THP-1 macrophages and SGBS adipocytes -- a new human in vitro model system of inflamed adipose tissue.
Fischer-Posovszky P.
Front. Endocrinol. 2:89.1-89.8(2011).
Anecdotal Information
- Have been flown in space on Cosmos-2044 to study the inhibition of phorbol ester-induced cell activation in microgravity (PubMed=1915666)