U-937Homo sapiens (Human)Cancer cell line
Also known as: U937, U 937
Quick Overview
Human histiocytic lymphoma cell line with macrophage-like properties
Detailed Summary
Research Applications
Key Characteristics
Basic Information
| Database ID | CVCL_0007 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Pleural effusion[UBERON:UBERON_0000175] |
Donor Information
| Age | 37 |
|---|---|
| Age Category | Adult |
| Sex | Male |
| Race | caucasian |
Disease Information
| Disease | Adult acute monocytic leukemia |
|---|---|
| Lineage | Myeloid |
| Subtype | Acute Myeloid Leukemia |
| OncoTree Code | AML |
DepMap Information
| Source Type | ATCC |
|---|---|
| Source ID | ACH-000406_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| Gene fusion | MLLT10 | PICALM-MLLT10, CALM-AF10 | - | - | from parent cell line U-937 |
| MutationSimple | PTEN | p.Gly129fs*51 (c.387_388insCGCC) | Hemizygous | - | from parent cell line U-937 |
| MutationSimple | PTPN11 | p.Gly60Arg (c.178G>C) | Heterozygous | - | from parent cell line U-937 |
| MutationSimple | TP53 | c.559+1G>A (p.Val173Trpfs*59) | Homozygous | Splice donor mutation | from parent cell line U-937 |
| MutationSimple | WT1 | p.Arg301Ter (c.901C>T) (p.Arg352Ter, c.1054C>T) (p.Arg374Ter, c.1120C>T) | Heterozygous | Somatic | from parent cell line U-937 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Loading gene expression data...
Publications
Initial characterization of the human central proteome.";
Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17.1-17.13(2011).
Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18.
Zamora L., Granada I., Buschbeck M.
Sci. Rep. 11:21145-21145(2021).
Detailed molecular cytogenetic characterisation of the myeloid cell line U937 reveals the fate of homologous chromosomes and shows that centromere capture is a feature of genome instability.
MacKinnon R.N., Peverall J., Campbell L.J., Wall M.
Mol. Cytogenet. 13:50.1-50.14(2020).
Quantitative proteomics of the Cancer Cell Line Encyclopedia.";
Sellers W.R., Gygi S.P.
Cell 180:387-402.e16(2020).
The LL-100 panel: 100 cell lines for blood cancer studies.";
MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.
Sci. Rep. 9:8218-8218(2019).
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Sellers W.R.
Nature 569:503-508(2019).
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
Cancer Res. 79:1263-1273(2019).
Screening human cell lines for viral infections applying RNA-Seq data analysis.
Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.
PLoS ONE 14:E0210404-E0210404(2019).
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
Yang H.H., Koeffler H.P.
BMC Cancer 18:940.1-940.13(2018).
THP-1 and U937 cells.";
Chanput W., Peters V., Wichers H.J.
(In book chapter) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx K., Cotter P., Lopez-Exposito I., Kleiveland C., Lea T., Mackie A., Requena T., Swiatecka D., Wichers H. (eds.); pp.147-159; Springer; Cham; Switzerland (2015).
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Loewer M., Sahin U., Castle J.C.
Genome Med. 7:118.1-118.7(2015).
A resource for cell line authentication, annotation and quality control.
Neve R.M.
Nature 520:307-311(2015).
A comprehensive transcriptional portrait of human cancer cell lines.
Settleman J., Seshagiri S., Zhang Z.-M.
Nat. Biotechnol. 33:306-312(2015).
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
Nature 483:603-607(2012).
Chromosome abnormalities in human tumor cells in culture.";
Biedler J.L.
(In book chapter) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York; USA (1975).
Characterization of a human derived macrophage-like cell line, U-M.";
Shinohara K., Kong Z.-L., Murakami H.
(In book chapter) Animal cell culture and production of biologicals; Sasaki R., Ikura K. (eds.); pp.181-186; Springer; Dordrecht; Netherlands (1991).
Severe disruption of the cytoskeleton and immunologically relevant surface molecules in a human macrophageal cell line in microgravity -- results of an in vitro experiment on board of the Shenzhou-8 space mission.
Zhuang F.-Y., Ullrich O.
Acta Astronaut. 94:277-292(2013).
Heterogeneity of cellular origins in human malignant lymphoma cell line derived from histo-monocytic lineage cells.
Morikawa S., Harada T., Katoh T.
(In book chapter) Cellular, molecular, genetic approaches to immunodiagnosis and immunotherapy. 8th International conference on labeled antibodies, Tokyo, November 1985; Kano K., Mori S., Sugisaki T., Torisu M. (eds.); pp.373-380; Karger; Basel; Switzerland (1988).
Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
Sundstrom C., Nilsson K.
Int. J. Cancer 17:565-577(1976).
Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.
Ralph P., Moore M.A.S., Nilsson K.
J. Exp. Med. 143:1528-1533(1976).
Frequent mutations in the p53 gene in human myeloid leukemia cell lines.
Ishikawa F., Takaku F., Yazaki Y., Hirai H.
Blood 79:2378-2383(1992).
Regulation of cellular trans-activating activities in two different promonocytic leukemia cell lines.
Chang K.S.-S., Liu W.-T., Josephs S.F.
Cancer Lett. 60:75-83(1991).
Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.
Nakano A., Harada T., Morikawa S., Kato Y.
Acta Pathol. Jpn. 40:107-115(1990).
Establishment of a macrophagelike cell line derived from U-937, human histiocytic lymphoma, grown serum-free.
Kong Z.-L., Miwa M., Murakami H., Shinohara K.
In Vitro Cell. Dev. Biol. 26:949-954(1990).
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1. carboxylic esterase.
Drexler H.G., Gaedicke G., Minowada J.
Leuk. Res. 9:209-229(1985).
Isoenzyme studies in human leukemia-lymphoma cell lines -- III. Beta-hexosaminidase (E.C. 3.2.1.30).
Drexler H.G., Gaedicke G., Minowada J.
Leuk. Res. 9:549-559(1985).
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Drexler H.G., Gaedicke G., Minowada J.
Leuk. Res. 9:537-548(1985).
Lineage infidelity of a human myelogenous leukemia cell line.";
Palumbo A., Minowada J., Erikson J., Croce C.M., Rovera G.
Blood 64:1059-1063(1984).
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984).
Cell line cross-contamination of U-937.";
Dorotinsky C.S., Hay R.J.
J. Leukoc. Biol. 57:804-804(1995).
Identification of a mutant human topoisomerase I with intact catalytic activity and resistance to 9-nitro-camptothecin.
Kufe D.W.
J. Biol. Chem. 269:2433-2439(1994).
Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.
Uppenkamp M.J., Nowrousian M.R., Seeber S., Opalka B.
Br. J. Haematol. 91:350-354(1995).
Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.
Imaizumi M., Endo M., Takano N., Konno T.
Leukemia 10:102-105(1996).
The (2;5)(p23;q35) translocation in cell lines derived from malignant lymphomas: absence of t(2;5) in Hodgkin-analogous cell lines.
Quentmeier H., Drexler H.G.
Leukemia 10:142-149(1996).
The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.
Bohlander S.K.
Proc. Natl. Acad. Sci. U.S.A. 93:4804-4809(1996).
Mechanisms of resistance in a human cell line exposed to sequential topoisomerase poisoning.
Pantazis P., Rubin E.H.
Cancer Res. 57:5100-5106(1997).
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Hirai H.
Jpn. J. Cancer Res. 89:33-39(1998).
Telomere analysis by fluorescence in situ hybridization and flow cytometry.
Roos G.
Nucleic Acids Res. 26:3651-3656(1998).
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Aizawa Y., Ueda R., Seto M.
Jpn. J. Cancer Res. 89:712-718(1998).
The kinetics of translocation and cellular quantity of protein kinase C in human leukocytes are modified during spaceflight.
Cazenave J.-P., Schmitt D.
FASEB J. 13 Suppl. 1:S23-S33(1999).
Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling.
Grever M.R., Byrd J.C., Botstein D., Brown P.O., Staudt L.M.
Nature 403:503-511(2000).
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Leuk. Res. 24:255-262(2000).
Mutational analysis of the tumour suppressor gene MMAC1/PTEN in malignant myeloid disorders.
Aggerholm A., Gronbaek K., Guldberg P., Hokland P.
Eur. J. Haematol. 65:109-113(2000).
Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Leuk. Res. 25:275-278(2001).
Short tandem repeat profiling provides an international reference standard for human cell lines.
Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).
The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH.
Young B.D., Jones L.K.
Cytogenet. Cell Genet. 94:9-14(2001).
Molecular cytogenetic analysis of the monoblastic cell line U937. karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization.
Cho Y.-H.
Cancer Genet. Cytogenet. 137:124-132(2002).
Cancer cell line identification by short tandem repeat profiling: power and limitations.
Schmidt S., Kofler R.
FASEB J. 19:434-436(2005).
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Fioretos T.
Leukemia 19:1042-1050(2005).
Development of an in vitro skin sensitization test using human cell lines: the human Cell Line Activation Test (h-CLAT). I. Optimization of the h-CLAT protocol.
Sakaguchi H., Miyazawa M., Ito Y., Suzuki H., Toyoda H.
Toxicol. In Vitro 20:767-773(2006).
Development of an in vitro skin sensitization test using human cell lines; human Cell Line Activation Test (h-CLAT). II. An inter-laboratory study of the h-CLAT.
Yoneyama K., Hirota M., Itagaki H., Toyoda H., Suzuki H.
Toxicol. In Vitro 20:774-784(2006).
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.
Leukemia 20:471-476(2006).
Anecdotal Information
- Have been flown in space on shuttle flight STS-76 and on Shenzhou-8 to study growth and cytoskeletal changes in microgravity (PubMed=10352142; DOI=10.1016/j.actaastro.2013.06.007)