SH-SY5YHomo sapiens (Human)Cancer cell line
Also known as: SH-SY5Y Parental, SY5Y, SK-SH-SY5Y, SHSY-5Y, SHSY5Y, SH-Sy5y, SH-SY5 (Occasionally.)
Quick Overview
SH-SY5Y is a human neuroblastoma cell line used in cancer and neurological research.
Detailed Summary
Research Applications
Key Characteristics
Basic Information
| Database ID | CVCL_0019 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Bone marrow[UBERON:UBERON_0002371] |
Donor Information
| Age | 4 |
|---|---|
| Age Category | Pediatric |
| Sex | Female |
| Subtype Features | MYC_Amplified |
Disease Information
| Disease | Neuroblastoma |
|---|---|
| Lineage | Peripheral Nervous System |
| Subtype | Neuroblastoma |
| OncoTree Code | NBL |
DepMap Information
| Source Type | ATCC |
|---|---|
| Source ID | ACH-001188_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | ALK | p.Phe1174Leu (c.3522C>A) | Unspecified | Only detected in 5% of the cells in early passages but increase to 50% in late passages | from parent cell line SH-EP007 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
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Publications
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Sellers W.R.
Nature 569:503-508(2019).
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
Cancer Res. 79:1263-1273(2019).
Identification of various cell culture models for the study of Zika virus.
Himmelsbach K., Hildt E.
World J. Virol. 7:10-20(2018).
An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.
Lindbjerg Andersen C., Nielsen M.L., Olsen J.V.
Cell Syst. 4:587-599.e4(2017).
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Sci. Data 4:170033-170033(2017).
The SH-SY5Y cell line in Parkinson's disease research: a systematic review.
Xicoy H., Wieringa B., Martens G.J.M.
Mol. Neurodegener. 12:10.1-10.11(2017).
Data for identification of GPI-anchored peptides and omega-sites in cancer cell lines.
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Data Brief 7:1302-1305(2016).
Identification of glycosylphosphatidylinositol-anchored proteins and omega-sites using TiO2-based affinity purification followed by hydrogen fluoride treatment.
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
J. Proteomics 139:77-83(2016).
A distinct gene expression signature characterizes human neuroblastoma cancer stem cells.
Ross R.A., Walton J.D., Han D., Guo H.-F., Cheung N.-K.V.
Stem Cell Res. 15:419-426(2015).
Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN in endosomes and lipid rafts.
George L., Comb M.J., Grimes M.L.
PLoS Comput. Biol. 11:e1004130.1-e1004130.33(2015).
N-linked glycan profiling in neuroblastoma cell lines.";
Volchenboum S.L.
J. Proteome Res. 14:2074-2081(2015).
Identification of cross-contamination in SH-SY5Y cell line.";
Jiang T., Wang H.-L.
Hum. Cell 27:176-178(2014).
Testing of SNS-032 in a panel of human neuroblastoma cell lines with acquired resistance to a broad range of drugs.
Fichtner I., Ghafourian T., Westermann F., Cinatl J. Jr.
Transl. Oncol. 6:685-696(2013).
Comparative proteomic analysis of human SH-SY5Y neuroblastoma cells under simulated microgravity.
Zhang Y.-Q., Wang H.-B., Lai C.-J., Wang L., Deng Y.-L.
Astrobiology 13:143-150(2013).
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
Nature 483:603-607(2012).
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.
Int. J. Cancer 131:1556-1568(2012).
NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.
Messiaen L.M., Versteeg R., Bernards R.
Cell 142:218-229(2010).
Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y.
Nishida Y., Adati N., Ozawa R., Maeda A., Sakaki Y., Takeda T.
BMC Res. Notes 1:95.1-95.11(2008).
1H MRS identifies specific metabolite profiles associated with MYCN-amplified and non-amplified tumour subtypes of neuroblastoma cell lines.
Edwards E.C., Strachan M.C., McMullan D.J., Wilkes T.M., Grundy R.G.
NMR Biomed. 20:692-700(2007).
Mutations in PIK3CA are infrequent in neuroblastoma.";
Dam V., Morgan B.T., Mazanek P., Hogarty M.D.
BMC Cancer 6:177.1-177.10(2006).
Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line.
Ghosh S.S., Gibson B.W.
Mitochondrion 1:161-179(2001).
Characteristics of stem cells from human neuroblastoma cell lines and in tumors.
Biedler J.L., Cheung N.-K.V., Ross R.A.
Neoplasia 6:838-845(2004).
Expression profiling of t(12;22) positive clear cell sarcoma of soft tissue cell lines reveals characteristic up-regulation of potential new marker genes including ERBB3.
Gabbert H.E., Poremba C.
Cancer Res. 64:3395-3405(2004).
Differential response of p53 target genes to p73 overexpression in SH-SY5Y neuroblastoma cell line.
Le Roux G., Haddada H., Benard J., Douc-Rasy S.
J. Cell Sci. 117:293-301(2004).
A corrected karyotype for the SH-SY5Y human neuroblastoma cell line.
Spengler B.A., Biedler J.L., Ross R.A.
Cancer Genet. Cytogenet. 138:177-178(2002).
Immunocytochemical analysis of cell lines derived from solid tumors.
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
J. Histochem. Cytochem. 49:1369-1378(2001).
Frequency of radiation-induced micronuclei in neuronal cells does not correlate with clonogenic survival.
Akudugu J.M., Slabbert J.P., Serafin A.M., Bohm L.
Radiat. Res. 153:62-67(2000).
Characterization of thread proteins expressed in neuroectodermal tumors.
Xu Y.-Y., Wands J.R., de la Monte S.M.
Cancer Res. 53:3823-3829(1993).
Coordinate morphological and biochemical interconversion of human neuroblastoma cells.
Ross R.A., Spengler B.A., Biedler J.L.
J. Natl. Cancer Inst. 71:741-747(1983).
Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.
Biedler J.L., Helson L., Spengler B.A.
Cancer Res. 33:2643-2652(1973).
Gene amplification-associated cytogenetic aberrations and protein changes in vincristine-resistant Chinese hamster, mouse, and human cells.
Meyers M.B., Spengler B.A., Chang T.-D., Melera P.W., Biedler J.L.
J. Cell Biol. 100:588-597(1985).
Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A.
Preis P.N., Saya H., Nadasdi L., Hochhaus G., Levin V.A., Sadee W.
Cancer Res. 48:6530-6534(1988).
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Ciccarone V.C., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.
Cancer Res. 49:219-225(1989).