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SK-BR-3Homo sapiens (Human)Cancer cell line

Also known as: SK-Br-3, Sk-Br-3, SK BR 03, SKBR-3, SKBr-3, SK-BR3, SKBr3, SkBr3, SKBR3, SKBR3B

🤖 AI SummaryBased on 12 publications

Quick Overview

Human breast cancer cell line used in cancer research and drug development.

Detailed Summary

SK-BR-3 is a human breast cancer cell line derived from a metastatic lesion. It is widely used in cancer research, particularly for studying HER2-positive breast cancers. The cell line exhibits characteristics of luminal B subtype and is known for its high expression of HER2 receptors. SK-BR-3 is utilized in studies related to drug resistance, gene expression, and molecular mechanisms of cancer progression. It has been employed in various research applications including proteomic analysis, drug screening, and investigation of oncogenic pathways. The cell line is also used to study the effects of targeted therapies and to identify potential biomarkers for cancer treatment.

Research Applications

Drug screeningProteomic analysisOncogenic pathway investigationHER2-positive breast cancer researchGene expression studiesMolecular mechanisms of cancer progressionTargeted therapy development

Key Characteristics

HER2-positiveLuminal B subtypeHigh HER2 receptor expressionMetastatic originUsed in drug resistance studies
Generated on 6/14/2025

Basic Information

Database IDCVCL_0033
SpeciesHomo sapiens (Human)
Tissue SourcePleural effusion[UBERON:UBERON_0000175]

Donor Information

Age43
Age CategoryAdult
SexFemale
Racecaucasian
Subtype FeaturesHER2+

Disease Information

DiseaseBreast adenocarcinoma
LineageBreast
SubtypeInvasive Breast Carcinoma
OncoTree CodeBRCA

DepMap Information

Source TypeATCC
Source IDACH-000017_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
Gene deletionCDH1-Heterozygous-CelloPub=CLPUB00696
MutationSimpleTP53p.Arg175His (c.524G>A)UnspecifiedSomatic mutation acquired during proliferationfrom parent cell line YCC-3

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X
CSF1PO
12
D13S317
11
D16S539
9
D18S51
10,13
D19S433
14
D21S11
30,30.2
D2S1338
20,25
D3S1358
17
D5S818
9
D7S820
9,12
D8S1179
11,12
FGA
20
Penta D
9,12
Penta E
10,11
TH01
8,9
TPOX
8,11
vWA
17
Gene Expression Profile
Gene expression levels and statistical distribution
Loading cohorts...
Full DepMap dataset with combined data across cell lines

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Publications

Essential gene profiles in breast, pancreatic, and ovarian cancer cells.

Rottapel R., Neel B.G., Moffat J.

Cancer Discov. 2:172-189(2012).

DOIPubMedPMC

HSP90 inhibitor, 17-DMAG, alone and in combination with lapatinib attenuates acquired lapatinib-resistance in ER-positive, HER2-overexpressing breast cancer cell line.

Lee H.J., Shin S., Kang J., Han K.-C., Kim Y.H., Bae J.-W., Park K.H.

Cancers (Basel) 12:2630.1-2630.16(2020).

DOIPubMedPMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOIPubMedPMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOIPubMedPMC

Complex rearrangements and oncogene amplifications revealed by long-read DNA and RNA sequencing of a breast cancer cell line.

McPherson J.D., Hicks J., McCombie W.R., Schatz M.C.

Genome Res. 28:1126-1135(2018).

DOIPubMedPMC

Enhancer transcription reveals subtype-specific gene expression programs controlling breast cancer pathogenesis.

Bedford M.T., Shi X.-B., Li W., Barton M.C., Dent S.Y.R., Kraus W.L.

Genome Res. 28:159-170(2018).

DOIPubMedPMC

Multidimensional phenotyping of breast cancer cell lines to guide preclinical research.

Lakhani S.R.

Breast Cancer Res. Treat. 167:289-301(2018).

DOIPubMed

Glycoproteins in claudin-low breast cancer cell lines have a unique expression profile.

Yen T.-Y., Bowen S., Yen R., Piryatinska A., Macher B.A., Timpe L.C.

J. Proteome Res. 16:1391-1400(2017).

DOIPubMedPMC

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

DOIPubMedPMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOIPubMedPMC

Redefining the breast cancer exosome proteome by tandem mass tag quantitative proteomics and multivariate cluster analysis.

Yang A.J.

Anal. Chem. 87:10462-10469(2015).

DOIPubMedPMC

Molecular portrait of breast-cancer-derived cell lines reveals poor similarity with tumors.

Cifani P., Kirik U., Waldemarson S., James P.

J. Proteome Res. 14:2819-2827(2015).

DOIPubMed

A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.

Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.

OncoImmunology 3:e954893.1-e954893.12(2014).

DOIPubMedPMC

The proteomic landscape of triple-negative breast cancer.";

Irie H.Y., Lee S.-I., Blau C.A., Villen J.

Cell Rep. 11:630-644(2015).

DOIPubMedPMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOIPubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOIPubMed

Differences and homologies of chromosomal alterations within and between breast cancer cell lines: a clustering analysis.

Ramirez-Clavijo S.R., Pasini B., Sapino A.

Mol. Cytogenet. 7:8.1-8.14(2014).

DOIPubMedPMC

More than apples and oranges -- detecting cancer with a fruit fly's antenna.

Capuano R., Di Natale C.

Sci. Rep. 4:3576-3576(2014).

DOIPubMedPMC

Modeling precision treatment of breast cancer.";

Collisson E.A., van 't Veer L.J., Spellman P.T., Gray J.W.

Genome Biol. 14:R110.1-R110.14(2013).

DOIPubMedPMC

Characterization of cell lines derived from breast cancers and normal mammary tissues for the study of the intrinsic molecular subtypes.

Harrell J.C., Roman E., Adamo B., Troester M.A., Perou C.M.

Breast Cancer Res. Treat. 142:237-255(2013).

DOIPubMedPMC

Glutamine sensitivity analysis identifies the xCT antiporter as a common triple-negative breast tumor therapeutic target.

McCormick F., Gray J.W.

Cancer Cell 24:450-465(2013).

DOIPubMedPMC

Cell surface-specific N-glycan profiling in breast cancer.";

Shi S.-L., Chen C.-Y., Li Y.

PLoS ONE 8:E72704-E72704(2013).

DOIPubMedPMC

A novel approach for characterizing microsatellite instability in cancer cells.

Lu Y.-H., Soong T.D., Elemento O.

PLoS ONE 8:E63056-E63056(2013).

DOIPubMedPMC

miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation-specific miRNAs.

Martens J.W.M.

Breast Cancer Res. 15:R33.1-R33.17(2013).

DOIPubMedPMC

Molecular characterisation of cell line models for triple-negative breast cancers.

Reis-Filho J.S., Tutt A.

BMC Genomics 13:619.1-619.14(2012).

DOIPubMedPMC

New human tumor cell lines.";

Fogh J., Trempe G.L.

(In book chapter) Human tumor cells in vitro; Fogh J. (eds.); pp.115-159; Springer; New York; USA (1975).

DOI

Cell lines from human breast.";

Leibovitz A.

(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York; USA (1994).

DOI

Breast cancer stem cells: tumourspheres and implications for therapy.";

Morrison B.J.

Thesis PhD (2010); Griffith University; Brisbane; Australia.

DOI

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

Fogh J., Fogh J.M., Orfeo T.

J. Natl. Cancer Inst. 59:221-226(1977).

DOIPubMed

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

Fogh J., Wright W.C., Loveless J.D.

J. Natl. Cancer Inst. 58:209-214(1977).

DOIPubMed

Human breast cancer in culture.";

Trempe G.L.

Recent Results Cancer Res. 57:33-41(1976).

DOIPubMed

Mutations in p53 as potential molecular markers for human breast cancer.

Runnebaum I.B., Nagarajan M., Bowman M., Soto D., Sukumar S.

Proc. Natl. Acad. Sci. U.S.A. 88:10657-10661(1991).

DOIPubMedPMC

Human tumor lines for cancer research.";

Fogh J.

Cancer Invest. 4:157-184(1986).

DOIPubMed

Cell culture quality control by rapid isoenzymatic characterization.

Halton D.M., Peterson W.D. Jr., Hukku B.

In Vitro 19:16-24(1983).

DOIPubMed

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

Gershwin M.E., Lentz D., Owens R.B.

Exp. Cell Biol. 52:361-370(1984).

DOIPubMed

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.

Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984).

DOIPubMedPMC

Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.

Wright W.C., Daniels W.P., Fogh J.

J. Natl. Cancer Inst. 66:239-247(1981).

DOIPubMed

HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.

Pollack M.S., Heagney S.D., Livingston P.O., Fogh J.

J. Natl. Cancer Inst. 66:1003-1012(1981).

DOIPubMed

Lack of relationship between CDK activity and G1 cyclin expression in breast cancer cells.

Sweeney K.J., Swarbrick A., Sutherland R.L., Musgrove E.A.

Oncogene 16:2865-2878(1998).

DOIPubMed

Chromosomal alterations in 15 breast cancer cell lines by comparative genomic hybridization and spectral karyotyping.

Isola J.J., Larsson C.

Genes Chromosomes Cancer 28:308-317(2000).

DOIPubMed

Comparative genomic hybridization analysis of 38 breast cancer cell lines: a basis for interpreting complementary DNA microarray data.

Gooden G.C., Ethier S.P., Kallioniemi A.H., Kallioniemi O.-P.

Cancer Res. 60:4519-4525(2000).

PubMed

Molecular cytogenetic analysis of breast cancer cell lines.";

Courtay-Cahen C., Roberts I., Theillet C., Caldas C., Edwards P.A.W.

Br. J. Cancer 83:1309-1317(2000).

DOIPubMedPMC

Aberrations of chromosome 8 in 16 breast cancer cell lines by comparative genomic hybridization, fluorescence in situ hybridization, and spectral karyotyping.

Isola J.J.

Cancer Genet. Cytogenet. 126:1-7(2001).

DOIPubMed

A recurrent chromosome translocation breakpoint in breast and pancreatic cancer cell lines targets the neuregulin/NRG1 gene.

Edwards P.A.W., Chaffanet M.

Genes Chromosomes Cancer 37:333-345(2003).

DOIPubMed

Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.

Edwards P.A.W., Caldas C.

Carcinogenesis 26:923-930(2005).

DOIPubMed

BRCA1 mutation analysis of 41 human breast cancer cell lines reveals three new deleterious mutants.

van den Ouweland A.M.W., Merajver S.D., Ethier S.P., Schutte M.

Cancer Res. 66:41-45(2006).

DOIPubMed

Comprehensive copy number profiles of breast cancer cell model genomes.

Shadeo A., Lam W.L.

Breast Cancer Res. 8:R9.1-R9.14(2006).

DOIPubMedPMC

Thirteen new p53 gene mutants identified among 41 human breast cancer cell lines.

Wasielewski M., Elstrodt F., Klijn J.G.M., Berns E.M.J.J., Schutte M.

Breast Cancer Res. Treat. 99:97-101(2006).

DOIPubMed

A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes.

Johnson M.D., Lippman M.E., Ethier S.P., Gazdar A.F., Gray J.W.

Cancer Cell 10:515-527(2006).

DOIPubMedPMC

High-resolution genomic profiles of breast cancer cell lines assessed by tiling BAC array comparative genomic hybridization.

Ringner M., Hoglund M., Borg A.

Genes Chromosomes Cancer 46:543-558(2007).

DOIPubMed

The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression.

Petersen O.W., Gray J.W., Bissell M.J.

Mol. Oncol. 1:84-96(2007).

DOIPubMedPMC

Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery.

Pollack J.R.

PLoS ONE 4:E6146-E6146(2009).

DOIPubMedPMC

Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines.

den Bakker M.A., Foekens J.A., Martens J.W.M., Schutte M.

Breast Cancer Res. Treat. 121:53-64(2010).

DOIPubMed

Systems-level modeling of cancer-fibroblast interaction.";

Finn S.P., Loda M., Mahmood U., Ramaswamy S.

PLoS ONE 4:E6888-E6888(2009).

DOIPubMedPMC

Breast cancer cell lines carry cell line-specific genomic alterations that are distinct from aberrations in breast cancer tissues: comparison of the CGH profiles between cancer cell lines and primary cancer tissues.

Yamamoto S., Oka M., Hirano T., Sasaki K.

BMC Cancer 10:15.1-15.10(2010).

DOIPubMedPMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOIPubMedPMC

Is HPV-18 present in human breast cancer cell lines?";

Peran I., Riegel A.T., Dai Y., Schlegel R., Liu X.

Br. J. Cancer 102:1549-1550(2010).

DOIPubMedPMC

Identification of fusion genes in breast cancer by paired-end RNA-sequencing.

Kallioniemi O.-P.

Genome Biol. 12:R6.1-R6.13(2011).

DOIPubMedPMC

Reassessment of estrogen receptor expression in human breast cancer cell lines.

Ford C.H.J., Al-Bader M., Al-Ayadhi B., Francis I.

Anticancer Res. 31:521-527(2011).

PubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOIPubMedPMC

Web Resources

en.wikipedia.orgcellosaurus.orgatcc.orgdpsc.ccbr.utoronto.caproteinatlas.orglincs.hms.harvard.eduthermofisher.commskcc.orgsynapse.orgtcpaportal.org

Anecdotal Information

  • Used in a study utilising the fruit fly's olfactory system to detect cancer cells (PubMed=24389870)