SK-MEL-1Homo sapiens (Human)Cancer cell line

Also known as: SK 1, SKMEL1, SkMEL-1, SKMEL-1, SK-Mel1, SK-Mel 1, SK Mel 1, SK-Mel-1

🤖 AI SummaryBased on 15 publications

Quick Overview

Human melanoma cell line with BRAF V600E mutation, used in cancer research.

Detailed Summary

SK-MEL-1 is a human melanoma cell line derived from a malignant melanoma. It is characterized by the presence of the BRAF V600E mutation, which is a common driver mutation in melanoma. This cell line is widely used in research to study the mechanisms of melanoma progression and to test the efficacy of targeted therapies, particularly those directed against the MAPK pathway. The cell line has been utilized in studies investigating the role of NF1 loss and its association with RAS activation and MEK dependence. Additionally, SK-MEL-1 has been employed in studies examining the impact of PTEN and RB1 tumor suppressor inactivation on RAF dependence in BRAF-mutant melanomas. The cell line is also part of large-scale genomic and transcriptomic studies, contributing to the understanding of cancer cell line diversity and the development of predictive models for drug response.

Research Applications

BRAF V600E mutation studiesMAPK pathway researchNF1 loss and RAS activationPTEN and RB1 inactivation studiesDrug response prediction models

Key Characteristics

BRAF V600E mutationNF1 lossPTEN and RB1 inactivationMEK dependence

Generated on 6/14/2025

Basic Information

Database ID	CVCL_0068
Species	Homo sapiens (Human)
Tissue Source	Thoracic lymph duct[UBERON:UBERON_0007644]

Donor Information

Age	29
Age Category	Adult
Sex	Male
Race	caucasian

Disease Information

Disease	Melanoma
Lineage	Skin
Subtype	Melanoma
OncoTree Code	MEL

DepMap Information

Source Type	ATCC
Source ID	ACH-000465_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	CTNNB1	p.Ser33Cys (c.98C>G)	Heterozygous	-	Unknown, Unknown, Unknown
MutationSimple	BRAF	p.Val600Glu (c.1799T>A)	Unspecified	-	PubMed=26214590

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

12,13

D13S317

D16S539

11,12

D18S51

13,16

D19S433

D21S11

29,32.2

D2S1338

19,23

D3S1358

14,16

D5S818

12,13

D7S820

D8S1179

13,16

FGA

17,20

Penta D

11,13

Penta E

7,21

TH01

TPOX

vWA

16,17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

DOI PubMed PMC

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOI PubMed

Loss of NF1 in cutaneous melanoma is associated with RAS activation and MEK dependence.

Rosen N., Solit D.B.

Cancer Res. 74:2340-2350(2014).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

Concurrent loss of the PTEN and RB1 tumor suppressors attenuates RAF dependence in melanomas harboring (V600E)BRAF.

Wolchok J.D., Houghton A.N., Solit D.B.

Oncogene 31:446-457(2012).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

Immunocytochemical analysis of cell lines derived from solid tumors.

Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.

J. Histochem. Cytochem. 49:1369-1378(2001).

DOI PubMed

Expression of hyaluronidase by tumor cells induces angiogenesis in vivo.

Markowitz S.D., Willson J.K.V., Sy M.-S.

Proc. Natl. Acad. Sci. U.S.A. 93:7832-7837(1996).

DOI PubMed PMC

Surface antigens of human melanoma cells defined by monoclonal antibodies. I. Biochemical characterization of two antigens found on cell lines and fresh tumors of diverse tissue origin.

Johnson J.P., Demmer-Dieckmann M., Meo T., Hadam M.R., Riethmuller G.

Eur. J. Immunol. 11:825-831(1981).

DOI PubMed

Polymorphic enzyme analysis of cultured human tumor cell lines.";

Dracopoli N.C., Fogh J.

J. Natl. Cancer Inst. 70:469-476(1983).

PubMed

Suspension culture of a pigment-producing cell line derived from a human malignant melanoma.

Oettgen H.F., Aoki T., Old L.J., Boyse E.A., de Harven E., Mills G.M.

J. Natl. Cancer Inst. 41:827-843(1968).

DOI PubMed

Human tumor lines for cancer research.";

Fogh J.

Cancer Invest. 4:157-184(1986).

DOI PubMed

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Cancer Res. 48:589-601(1988).

PubMed

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

Fogh J., Wright W.C., Loveless J.D.

J. Natl. Cancer Inst. 58:209-214(1977).

DOI PubMed

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

Fogh J., Fogh J.M., Orfeo T.

J. Natl. Cancer Inst. 59:221-226(1977).

DOI PubMed

Web Resources

mskcc.org tcpaportal.org