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NALM-1Homo sapiens (Human)Cancer cell line

Also known as: Nalm1, NALM1, NALM 1, Nalm-1

🤖 AI SummaryBased on 15 publications

Quick Overview

Human B-cell precursor leukemia cell line with Ph+ and JAK2 mutations.

Detailed Summary

NALM-1 is a human B-cell precursor leukemia cell line established from the peripheral blood of a patient with chronic myeloid leukemia (CML) in blast crisis. It is characterized by the presence of the Philadelphia (Ph) chromosome, which results from the t(9;22)(q34;q11) translocation, leading to the BCR-ABL fusion gene. This cell line is also noted for harboring the JAK2 V617F mutation, which is associated with myeloproliferative disorders. NALM-1 is widely used in research for studying the molecular mechanisms of CML and related leukemias, including the role of BCR-ABL and JAK2 mutations in leukemogenesis. The cell line is also utilized in drug sensitivity studies and for understanding the genetic alterations that contribute to leukemia progression.

Research Applications

Molecular mechanisms of CMLBCR-ABL fusion gene analysisJAK2 V617F mutation studiesDrug sensitivity testingGenetic alterations in leukemia

Key Characteristics

Ph+ chromosomeBCR-ABL fusion geneJAK2 V617F mutationB-cell precursor lineage
Generated on 6/14/2025

Basic Information

Database IDCVCL_0091
SpeciesHomo sapiens (Human)
Tissue SourcePeripheral blood[UBERON:UBERON_0000178]

Donor Information

Age3
Age CategoryPediatric
SexFemale
Subtype FeaturesBCR-ABL1 positive

Disease Information

DiseaseChronic myeloid leukemia
LineageMyeloid
SubtypeChronic Myeloid Leukemia, BCR-ABL1+
OncoTree CodeCMLBCRABL1

DepMap Information

Source TypeDSMZ
Source IDACH-000462_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
Gene fusionABL1BCR-ABL1, BCR-ABL-BCR exon 1 fused to ABL1 exon 2PubMed=10576511, PubMed=10071072, PubMed=8751477

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X
CSF1PO
10,12
D13S317
11,12
D16S539
11,15
D18S51
11,15
D19S433
11,15
D21S11
29,30
D2S1338
17,20
D3S1358
15,17
D5S818
11
D7S820
10,11
D8S1179
14,15
FGA
23,26
Penta D
3
Penta E
8,9
TH01
7,9
TPOX
9,11
vWA
15,17
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines

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Publications

Glucocorticoid receptor gene mutations confer glucocorticoid resistance in B-cell precursor acute lymphoblastic leukemia.

Minegishi M., Iwamoto S., Sugita K., Inukai T.

J. Steroid Biochem. Mol. Biol. 218:106068.1-106068.12(2022).

DOIPubMed

The LL-100 panel: 100 cell lines for blood cancer studies.";

MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.

Sci. Rep. 9:8218-8218(2019).

DOIPubMedPMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOIPubMedPMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOIPubMedPMC

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

DOIPubMedPMC

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

DOIPubMedPMC

Copy number abnormality of acute lymphoblastic leukemia cell lines based on their genetic subtypes.

Sugita K., Hosoi H.

Int. J. Hematol. 108:312-318(2018).

DOIPubMed

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOIPubMedPMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOIPubMedPMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOIPubMedPMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOIPubMedPMC

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

Leukemia 20:471-476(2006).

DOIPubMed

Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.

Fioretos T.

Leukemia 19:1042-1050(2005).

DOIPubMed

Re-analysis of the cell line NALM-1 karyotype by GTG-banding, spectral karyotyping, and whole chromosome painting.

Pelz A.-F., Weilepp G., Wieacker P.F.

Cancer Genet. Cytogenet. 156:59-61(2005).

DOIPubMed

TNF-related apoptosis-inducing ligand (TRAIL) frequently induces apoptosis in Philadelphia chromosome-positive leukemia cells.

Koyama-Okazaki T., Suzuki T., Sugita K., Nakazawa S.

Blood 101:3658-3667(2003).

DOIPubMed

ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.";

Uphoff C.C., Habig S., Fombonne S., Matsuo Y., Drexler H.G.

Leuk. Res. 23:1055-1060(1999).

DOIPubMed

Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.

Drexler H.G., MacLeod R.A.F., Uphoff C.C.

Leuk. Res. 23:207-215(1999).

DOIPubMed

Establishment and characterization of human B cell precursor-leukemia cell lines.

Matsuo Y., Drexler H.G.

Leuk. Res. 22:567-579(1998).

DOIPubMed

Occurrence of TEL-AML1 fusion resulting from (12;21) translocation in human early B-lineage leukemia cell lines.

Janssen J.W.G., Drexler H.G.

Leukemia 11:441-447(1997).

DOIPubMed

Heterotransplantation and clonal growth of human Ph'-chromosome-positive leukemia-cell (NALM-1) and B-cell leukemia-cell (BALM-2) lines.

Kubonishi I., Freeman A.I., Minowada J.

Cancer 45:2324-2329(1980).

DOIPubMed

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.

Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984).

DOIPubMedPMC

Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:537-548(1985).

DOIPubMed

Ph positive CML cell lines.";

Keating A.

Baillieres Clin. Haematol. 1:1021-1029(1987).

DOIPubMed

Isoenzyme studies in human leukemia-lymphoma cell lines -- III. Beta-hexosaminidase (E.C. 3.2.1.30).

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:549-559(1985).

DOIPubMed

Isoenzyme studies in human leukemia-lymphoma cell lines -- 1. carboxylic esterase.

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:209-229(1985).

DOIPubMed

Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.

Nakano A., Harada T., Morikawa S., Kato Y.

Acta Pathol. Jpn. 40:107-115(1990).

DOIPubMed

Cytogenetic study of a new Ph1-positive cell line (NALM-1).";

Sonta S.-i., Minowada J., Tsubota T., Sandberg A.A.

J. Natl. Cancer Inst. 59:833-837(1977).

DOIPubMed

A non-T, non-B human leukemia cell line (NALM-1): establishment of the cell line and presence of leukemia-associated antigens.

Minowada J., Tsubota T., Greaves M.F., Walters T.R.

J. Natl. Cancer Inst. 59:83-87(1977).

DOIPubMed

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI