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SEMHomo sapiens (Human)Cancer cell line

🤖 AI SummaryBased on 11 publications

Quick Overview

Human acute lymphoblastic leukemia cell line with t(4;11) translocation

Detailed Summary

The SEM cell line is a human acute lymphoblastic leukemia cell line established from the peripheral blood of a 5-year-old girl in relapse with t(4;11) chromosomal rearrangement. It exhibits a biphenotypic immunophenotype, expressing both B-cell and myeloid markers. The cell line is known for its ability to respond to interleukin-7, which promotes its growth in culture. SEM cells carry the t(4;11)(q21;q23) translocation, which results in the fusion of the MLL gene on chromosome 11 with the AF-4 gene on chromosome 4. This translocation is associated with a poor prognosis in patients with acute lymphoblastic leukemia. The SEM cell line is widely used in research to study the molecular mechanisms of leukemogenesis and the effects of cytokines on leukemia cell proliferation.

Research Applications

Study of chromosomal translocations in leukemiaInvestigation of MLL-AF4 fusion geneAnalysis of cytokine effects on leukemia cell growthModel for t(4;11) translocation in acute lymphoblastic leukemia

Key Characteristics

Biphenotypic immunophenotypet(4;11) chromosomal translocationResponse to interleukin-7Expression of both B-cell and myeloid markers

Generated on 6/14/2025

Basic Information

Database ID	CVCL_0095
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age	5
Age Category	Pediatric
Sex	Female
Subtype Features	KMT2A

Disease Information

Disease	B-lymphoblastic leukemia/lymphoma with t(v;11q23.3)
Lineage	Lymphoid
Subtype	B-Lymphoblastic Leukemia/Lymphoma with t(v;11q23.3);KMT2A Rearranged
OncoTree Code	BLLKMT2A

DepMap Information

Source Type	DSMZ
Source ID	ACH-000782_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg248Gln (c.743G>A)	Unspecified	Somatic mutation acquired during proliferation	PubMed=20575032
MutationSimple	CDKN2A	p.His83Tyr (c.247C>T) (p.Ala97Val, c.290C>T)	Unspecified	-	PubMed=11787853
Gene fusion	AFF1	KMT2A-AFF1, MLL-AFF1, ALL-1/AF4	-	In frame	from parent cell line SEMK2

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X

CSF1PO

11,12

D13S317

14

D16S539

8,10

D18S51

16,17

D19S433

13,14

D21S11

29,30,31

D2S1338

17,24

D3S1358

14,17

D5S818

12

D7S820

10,12

D8S1179

11,14

FGA

21,25

Penta D

11,14

Penta E

10,11

TH01

6,10

TPOX

8

vWA

17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines.

Lehtio J., Vesterlund M., Jafari R.

Nat. Commun. 13:1691.1-1691.19(2022).

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

The LL-100 panel: 100 cell lines for blood cancer studies.";

MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.

Sci. Rep. 9:8218-8218(2019).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

Survey of activated FLT3 signaling in leukemia.";

Druker B.J., Heinrich M.C., Rush J., Polakiewicz R.D.

PLoS ONE 6:E19169-E19169(2011).

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

Malignant hematopoietic cell lines: in vitro models for the study of MLL gene alterations.

Drexler H.G., Quentmeier H., MacLeod R.A.F.

Leukemia 18:227-232(2004).

Fine structure of translocation breakpoints in leukemic blasts with chromosomal translocation t(4;11): the DNA damage-repair model of translocation.

Marschalek R.

Oncogene 17:3035-3044(1998).

Occurrence of TEL-AML1 fusion resulting from (12;21) translocation in human early B-lineage leukemia cell lines.

Janssen J.W.G., Drexler H.G.

Leukemia 11:441-447(1997).

The acute lymphoblastic leukaemia cell line SEM with t(4;11) chromosomal rearrangement is biphenotypic and responsive to interleukin-7.

Beck J.-D.

Br. J. Haematol. 86:275-283(1994).

Molecular analysis of the chromosomal breakpoint and fusion transcripts in the acute lymphoblastic SEM cell line with chromosomal translocation t(4;11).

Zweckbronner I., Beck J.-D., Fey G.H.

Br. J. Haematol. 90:308-320(1995).

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).