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SKM-1Homo sapiens (Human)Cancer cell line

Also known as: SKM1

🤖 AI SummaryBased on 9 publications

Quick Overview

SKM-1 is a human myeloid cell line derived from a patient with myelodysplastic syndrome (MDS) progressing to acute myelogenous ...

Detailed Summary

SKM-1 is a human myeloid cell line established from the peripheral blood of a 76-year-old Japanese male patient with monoblastic leukemia following myelodysplastic syndrome (MDS). The cell line was derived during disease progression when the patient lost multiple point mutations of ras genes and acquired chromosomal abnormalities involving the short arm of chromosome 17. SKM-1 cells exhibit characteristics of immature monoblasts, with a high nuclear-to-cytoplasmic ratio and positive staining for butyrate esterase. The cells express myelomonocyte-specific antigens such as CD4, CD13, CD33, HLA-DR, and CD11b. Cytogenetic analysis revealed a karyotype of 46,XY, del(9)q13:q22), der(17)t(17:7)p13:z. SKM-1 cells show mutations in the p53 gene, including a missense mutation at codon 327 and a silent mutation at codon 158. These genetic alterations suggest a role for p53 in the progression from MDS to myelogenous leukemia. The cell line has been used to study the molecular mechanisms underlying the transformation of MDS to AML and the role of p53 in clonal evolution.

Research Applications

Study of myelodysplastic syndrome (MDS) progression to acute myelogenous leukemia (AML)Investigation of p53 gene mutations and their role in clonal evolutionAnalysis of chromosomal abnormalities and their impact on disease progressionCharacterization of myelomonocyte-specific antigens and cell surface markers

Key Characteristics

Established from a patient with MDS progressing to AMLLoss of ras gene mutations during disease progressionAcquisition of chromosomal abnormalities involving 17pMutations in the p53 gene (missense and silent mutations)Expression of myelomonocyte-specific antigens (CD4, CD13, CD33, HLA-DR, CD11b)Karyotype: 46,XY, del(9)q13:q22), der(17)t(17:7)p13:z

Generated on 6/14/2025

Basic Information

Database ID	CVCL_0098
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age	76
Age Category	Adult
Sex	Male
Race	asian

Disease Information

Disease	Acute myeloid leukemia
Lineage	Myeloid
Subtype	Acute Myeloid Leukemia
OncoTree Code	AML

DepMap Information

Source Type	HSRRB
Source ID	ACH-000373_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg248Gln (c.743G>A)	Unspecified	Somatic mutation acquired during proliferation	PubMed=20575032
MutationSimple	KRAS	p.Lys117Asn (c.351A>C)	Homozygous	-	from parent cell line SKM-1
MutationSimple	EZH2	p.Tyr646Cys (c.1937A>G)	Heterozygous	-	Unknown, Unknown
MutationSimple	BCORL1	c.4619-1G>A	Homozygous	Splice acceptor mutation	from parent cell line SKM-1
MutationSimple	ASXL1	p.Tyr591Ter (c.1773C>A)	Homozygous	-	PubMed=27750403

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X

CSF1PO

10,12

D13S317

11,13

D16S539

9,11

D18S51

14,15

D19S433

14,15

D21S11

31.2,32.2

D2S1338

19,23

D3S1358

18

D5S818

10,12

D7S820

10,11

D8S1179

11,13

FGA

22,23

Penta D

9,12

Penta E

15,19

TH01

7,9

TPOX

8,11

vWA

18

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

Immunophenotypic, cytogenetic, and mutational characterization of cell lines derived from myelodysplastic syndrome patients after progression to acute myeloid leukemia.

Campos D., Granada I., Junca J., Drexler H.G., Sole F., Buschbeck M.

Genes Chromosomes Cancer 56:243-252(2017).

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

Somatic mutations in the transcriptional corepressor gene BCORL1 in adult acute myelogenous leukemia.

Vogelstein B., Kinzler K.W., Papadopoulos N., Malek S.N.

Blood 118:5914-5917(2011).

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

Leukemia 20:471-476(2006).

The SKM-1 leukemic cell line established from a patient with progression to myelomonocytic leukemia in myelodysplastic syndrome (MDS)-contribution to better understanding of MDS.

Nakagawa T., Matozaki S.

Leuk. Lymphoma 17:335-339(1995).

Establishment of a leukaemic cell line from a patient with acquisition of chromosomal abnormalities during disease progression in myelodysplastic syndrome.

Kawaguchi R., Yokoyama Y., Hikiji K., Isobe T., Chihara K.

Br. J. Haematol. 85:469-476(1993).

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).