CMK-11-5Homo sapiens (Human)Cancer cell line

Also known as: CMK115, CMK11-5, CMK 11-5

🤖 AI SummaryBased on 4 publications

Quick Overview

Human acute megakaryoblastic leukemia cell line with p53/FXR2 fusion gene

Detailed Summary

CMK-11-5 is a human acute megakaryoblastic leukemia cell line derived from a Down syndrome patient. This cell line is notable for expressing a novel p53/FXR2 chimeric gene resulting from an interstitial deletion on chromosome 17. The p53/FXR2 fusion protein lacks the DNA-binding domain of wild-type p53 and is localized in the cytoplasm rather than the nucleus. Research has shown that this fusion protein fails to activate the p21 WAF-1/CIP1 promoter, indicating a loss of transcriptional activity. The cell line is used in studies related to leukemia biology, genetic mutations, and drug response mechanisms. It has also been utilized in investigations of platelet calcium signaling and megakaryocyte function.

Research Applications

Leukemia biologyGenetic mutationsDrug response mechanismsPlatelet calcium signalingMegakaryocyte function

Key Characteristics

p53/FXR2 chimeric geneCytoplasmic localization of fusion proteinLoss of p21 WAF-1/CIP1 promoter activation

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0217
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age Category	Pediatric
Sex	Male

Disease Information

Disease	Acute megakaryoblastic leukemia in Down syndrome
Lineage	Myeloid
Subtype	Myeloid Leukemia Associated with Down Syndrome
OncoTree Code	MLADS

DepMap Information

Source Type	HSRRB
Source ID	ACH-001036_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Asp49His (c.145G>C)	Heterozygous	-	from parent cell line Ishikawa
Gene fusion	FXR2	TP53-FXR2	-	-	PubMed=16778363

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

11,13

D13S317

D16S539

9,11

D5S818

11,12

D7S820

11,12

TH01

TPOX

9,11

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Establishment of a megakaryoblastic cell line for conventional assessment of platelet calcium signaling.

Yatomi Y., Ohmori T.

Int. J. Hematol. 111:786-794(2020).

DOI PubMed

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

Cloning and characterization of the novel chimeric gene p53/FXR2 in the acute megakaryoblastic leukemia cell line CMK11-5.

Kanezaki R., Toki T., Xu G., Narayanan R., Ito E.

Tohoku J. Exp. Med. 209:169-180(2006).

DOI PubMed

Human immunodeficiency virus infection of megakaryocytic cells.";

Sakaguchi M., Sato T., Groopman J.E.

Blood 77:481-485(1991).

DOI PubMed

Ultrastructural analysis of platelet-like particles from a human megakaryocytic leukemia cell line (CMK 11-5).

Ryo R., Sato T.

Int. J. Hematol. 56:67-78(1992).

PubMed

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI