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DLD-1Homo sapiens (Human)Cancer cell line

Also known as: DLD 1, DLD1, CoCL3, DLD

🤖 AI SummaryBased on 16 publications

Quick Overview

Human colon epithelial cell line with known APC mutations and drug sensitivity profiles.

Detailed Summary

DLD-1 is a human colon epithelial cell line derived from a colorectal cancer tumor. It is widely used in cancer research due to its well-characterized genetic profile, including specific mutations in the APC gene, which is critical in the Wnt/β-catenin signaling pathway. Research on DLD-1 has contributed to understanding the molecular mechanisms of colorectal cancer, particularly in relation to drug sensitivity and resistance. The cell line is notable for its use in studies involving tankyrase inhibitors and other targeted therapies, as well as in investigations of genetic diversity and its impact on treatment outcomes. DLD-1 is also utilized in studies examining the role of APC mutations in cancer progression and therapeutic response.

Research Applications

Molecular mechanisms of colorectal cancerDrug sensitivity and resistance studiesAPC mutation analysisTankyrase inhibitor researchGenetic diversity in cancer treatment

Key Characteristics

APC gene mutationsWnt/β-catenin pathway involvementDrug sensitivity profilesUsed in targeted therapy research
Generated on 6/15/2025

Basic Information

Database IDCVCL_0248
SpeciesHomo sapiens (Human)
Tissue SourceColon[UBERON:UBERON_0001155]

Donor Information

Age67
Age CategoryAdult
SexMale

Disease Information

DiseaseColon adenocarcinoma
LineageBowel
SubtypeColon Adenocarcinoma
OncoTree CodeCOAD

DepMap Information

Source TypeATCC
Source IDACH-001061_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
MutationSimpleACVR2Ap.Lys437Argfs*5 (c.1310delA)Homozygous-PubMed=12615714
MutationSimpleAPCp.Arg727Met (c.2180G>T)Heterozygous-from parent cell line HCT 15
MutationSimpleAPCp.Lys993Asn (c.2979G>T)Heterozygous-from parent cell line HCT 15
MutationSimpleAPCp.Ile1417Leufs*2 (c.4248delC)Heterozygous-from parent cell line HCT 15
MutationSimpleAPCp.Arg2166Ter (c.6496C>T)Heterozygous-from parent cell line HCT 15
MutationSimpleB2Mc.68-1G>THeterozygousSplice acceptor mutationfrom parent cell line HCT 15
MutationSimpleB2Mp.Tyr30Ter (c.90C>A)Heterozygous-from parent cell line HCT 15
MutationSimpleEP300p.Glu1014Ter (c.3040G>T)Heterozygous-PubMed=10700188
MutationSimpleKRASp.Gly13Asp (c.38G>A)HeterozygousSomaticfrom parent cell line MDA-MB-231
MutationSimplePIK3CAp.Glu545Lys (c.1633G>A)Heterozygous-from parent cell line MCF-7
MutationSimplePIK3CAp.Asp549Asn (c.1645G>A)Heterozygous-from parent cell line HCT 15
MutationSimpleTGFBR2p.Lys128Serfs*35 (c.383delA)Homozygous-PubMed=12615714
MutationSimpleTP53p.Ser241Phe (c.722C>T)Unspecified-PubMed=23851445, PubMed=17260012

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X,Y
CSF1PO
11,12
D12S391
19,22
D13S317
8,11
D16S539
12,13
D18S51
11,17
D19S433
14,16
D21S11
29,32.2
D2S1338
17,25
D2S441
13,15
D3S1358
17
D5S818
13
D6S1043
11,13
D7S820
10,12
D8S1179
15
FGA
22
Penta D
9,14
Penta E
7,14
TH01
7,9.3
TPOX
8,11
vWA
18,19
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines

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Publications

5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.

Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.

Br. J. Cancer 103:340-346(2010).

DOIPubMedPMC

Comparison of different colorectal cancer with liver metastases models using six colorectal cancer cell lines.

Xu Y.-T., Zhang L., Wang Q.-L., Zheng M.-J.

Pathol. Oncol. Res. 26:2177-2183(2020).

DOIPubMed

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOIPubMedPMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOIPubMedPMC

Pharmacoproteomic characterisation of human colon and rectal cancer.

Weichert W., Knapp S., Feller S.M., Kuster B.

Mol. Syst. Biol. 13:951-951(2017).

DOIPubMedPMC

Multi-omics of 34 colorectal cancer cell lines -- a resource for biomedical studies.

Myklebost O., Skotheim R.I., Sveen A., Lothe R.A.

Mol. Cancer 16:116.1-116.16(2017).

DOIPubMedPMC

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

DOIPubMedPMC

A novel RNA sequencing data analysis method for cell line authentication.

Uhlen M., Al-Khalili Szigyarto C.

PLoS ONE 12:E0171435-E0171435(2017).

DOIPubMedPMC

APC mutations as a potential biomarker for sensitivity to tankyrase inhibitors in colorectal cancer.

Nagayama S., Fujita N., Sugimoto Y., Seimiya H.

Mol. Cancer Ther. 16:752-762(2017).

DOIPubMed

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOIPubMedPMC

N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.

Tollenaar R.A.E.M., Rombouts Y., Wuhrer M.

Mol. Cell. Proteomics 15:124-140(2016).

DOIPubMedPMC

Genome wide expression profiling of cancer cell lines cultured in microgravity reveals significant dysregulation of cell cycle and microRNA gene networks.

Kumar R., Verma R.S.

PLoS ONE 10:E0135958-E0135958(2015).

DOIPubMedPMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOIPubMedPMC

A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.

Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.

OncoImmunology 3:e954893.1-e954893.12(2014).

DOIPubMedPMC

Highly expressed genes in rapidly proliferating tumor cells as new targets for colorectal cancer treatment.

Sanchez A., Schwartz S. Jr., Bilic J., Mariadason J.M., Arango D.

Clin. Cancer Res. 21:3695-3704(2015).

DOIPubMed

The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.

Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.

Nat. Commun. 6:7002.1-7002.10(2015).

DOIPubMed

Feasibility of label-free phosphoproteomics and application to base-line signaling of colorectal cancer cell lines.

Pham T.V., Ishihama Y., Verheul H.M.W., Jimenez C.R.

J. Proteomics 127:247-258(2015).

DOIPubMed

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOIPubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOIPubMed

Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.

Mariadason J.M., Sieber O.M.

Cancer Res. 74:3238-3247(2014).

DOIPubMed

Epigenetic and genetic features of 24 colon cancer cell lines.";

Hektoen M., Lind G.E., Lothe R.A.

Oncogenesis 2:e71.1-e71.8(2013).

DOIPubMedPMC

Subtypes of primary colorectal tumors correlate with response to targeted treatment in colorectal cell lines.

Orphanides G., French T., Wessels L.F.A.

BMC Med. Genomics 5:66.1-66.15(2012).

DOIPubMedPMC

DCK is frequently inactivated in acquired gemcitabine-resistant human cancer cells.

Ishida M., Motoi F., Egawa S., Unno M., Horii A.

Biochem. Biophys. Res. Commun. 421:98-104(2012).

DOIPubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOIPubMedPMC

N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells.

Dexter D.L., Barbosa J.A., Calabresi P.

Cancer Res. 39:1020-1025(1979).

PubMed

Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.

Gray-Goodrich M., Campbell H., Mayo J.G., Boyd M.R.

J. Natl. Cancer Inst. 83:757-766(1991).

DOIPubMed

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Cancer Res. 48:589-601(1988).

PubMed

Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins.

Chen T.-R., Hay R.J., Macy M.L.

Cancer Genet. Cytogenet. 10:351-362(1983).

DOIPubMed

Activities of purine-metabolizing enzymes in human colon carcinoma cell lines and xenograft tumors.

Rogler-Brown T.L., Calabresi P., Parks R.E. Jr.

Biochem. Pharmacol. 30:793-798(1981).

DOIPubMed

DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin.

Chen T.-R., Dorotinsky C.S., McGuire L.J., Macy M.L., Hay R.J.

Cancer Genet. Cytogenet. 81:103-108(1995).

DOIPubMed

Increased incidence of p53 mutations is associated with hepatic metastasis in colorectal neoplastic progression.

Steele G., Summerhayes I.C.

Oncogene 11:647-652(1995).

PubMed

Expression of cytoskeletal-associated protein tyrosine phosphatase PTPH1 mRNA in human hepatocellular carcinoma.

Yachi A.

J. Gastroenterol. 29:727-732(1994).

DOIPubMed

Mutations and altered expression of p16INK4 in human cancer.";

Harris C.C.

Proc. Natl. Acad. Sci. U.S.A. 91:11045-11049(1994).

DOIPubMedPMC

Beta 2-microglobulin gene mutations: a study of established colorectal cell lines and fresh tumors.

Bicknell D.C., Rowan A.J., Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 91:4751-4755(1994).

DOIPubMedPMC

Liver metastases with 10 human colon carcinoma cell lines in nude mice and association with carcinoembryonic antigen production.

Tibbetts L.M., Doremus C.M., Tzanakakis G.N., Vezeridis M.P.

Cancer 71:315-321(1993).

DOIPubMed

Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.

Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 90:2842-2845(1993).

DOIPubMedPMC

Screening the p53 status of human cell lines using a yeast functional assay.

Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.

Mol. Carcinog. 19:243-253(1997).

DOIPubMed

Beta-catenin mutations in cell lines established from human colorectal cancers.

Ilyas M., Tomlinson I.P.M., Rowan A.J., Pignatelli M., Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 94:10330-10334(1997).

DOIPubMedPMC

Mutational analysis of the APC/beta-catenin/Tcf pathway in colorectal cancer.

Sparks A.B., Morin P.J., Vogelstein B., Kinzler K.W.

Cancer Res. 58:1130-1134(1998).

PubMed

Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?

Mareel M.M.

Cancer Genet. Cytogenet. 107:76-79(1998).

DOIPubMed

Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations.

Neumann T., Jauho A., Auer G., Ried T.

Genes Chromosomes Cancer 27:183-190(2000).

DOIPubMedPMC

Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.

Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.

Eur. J. Cancer 35:1724-1729(1999).

DOIPubMed

Mutations truncating the EP300 acetylase in human cancers.";

Delhanty J.D.A., Ponder B.A.J., Kouzarides T., Caldas C.

Nat. Genet. 24:300-303(2000).

DOIPubMed

Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement.

Bicknell D.C., Bodmer W.F., Arends M.J., Wyllie A.H., Edwards P.A.W.

Proc. Natl. Acad. Sci. U.S.A. 98:2538-2543(2001).

DOIPubMedPMC

Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.

Wolf E., Gabius H.-J.

J. Cancer Res. Clin. Oncol. 127:375-386(2001).

DOIPubMed

Short tandem repeat profiling provides an international reference standard for human cell lines.

Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).

DOIPubMedPMC

Immunocytochemical analysis of cell lines derived from solid tumors.

Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.

J. Histochem. Cytochem. 49:1369-1378(2001).

DOIPubMed

Mutations of the BRAF gene in human cancer.";

Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.

Nature 417:949-954(2002).

DOIPubMed

Evidence of selection for clones having genetic inactivation of the activin A type II receptor (ACVR2) gene in gastrointestinal cancers.

Willson J.K.V., Yeo C.J., Hruban R.H., Kern S.E.

Cancer Res. 63:994-999(2003).

PubMed

Analysis of p53 mutations and their expression in 56 colorectal cancer cell lines.

Liu Y., Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006).

DOIPubMedPMC

Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.

Garcia-Foncillas J.

Mol. Cancer 5:29.1-29.10(2006).

DOIPubMedPMC

Cell growth, global phosphotyrosine elevation, and c-Met phosphorylation through Src family kinases in colorectal cancer cells.

Emaduddin M., Bicknell D.C., Bodmer W.F., Feller S.M.

Proc. Natl. Acad. Sci. U.S.A. 105:2358-2362(2008).

DOIPubMedPMC

Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines.

Camps J., McNeil N.E., Difilippantonio M.J., Ried T.

Genes Chromosomes Cancer 49:204-223(2010).

DOIPubMedPMC

Genomic and biological characterization of exon 4 KRAS mutations in human cancer.

Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.

Cancer Res. 70:5901-5911(2010).

DOIPubMedPMC

Web Resources

dtp.cancer.govcellosaurus.orgtcpaportal.org