Back to Cell Types

Hs 294THomo sapiens (Human)Cancer cell line

Also known as: HS294, Hs294, HS294T, Hs294T, HS-294, HS-294-T, Hs-294T, HS-294T, HTB-140 (Based on the ATCC catalog number.), HTB140 (Based on the ATCC catalog number.)

🤖 AI SummaryBased on 12 publications

Hs294T

Quick Overview

Human melanoma cell line with metastatic potential and specific molecular characteristics.

Detailed Summary

The Hs294T cell line is a human melanoma cell line derived from a metastatic tumor. It exhibits characteristics associated with malignant transformation, including abnormal morphology, high growth rate, and tumorigenicity in immunosuppressed mice. This cell line has been used in studies investigating the role of hyaluronidase in tumor metastasis and the expression of nerve growth factor (NGF) receptors. Additionally, it has been utilized in research on BRAF mutations and their implications in melanoma progression. The cell line shows variability in melanin production and exhibits distinct growth patterns in different culture conditions.

Research Applications

Investigation of hyaluronidase expression and its role in tumor metastasisAnalysis of NGF receptor expression and signalingStudy of BRAF mutations and their impact on melanoma progressionEvaluation of tumorigenicity in immunosuppressed miceCharacterization of cell morphology and growth properties

Key Characteristics

Metastatic originAbnormal morphologyHigh growth rateTumorigenic in vivoVariable melanin productionExpression of NGF receptorsBRAF mutations

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0331
Species	Homo sapiens (Human)
Tissue Source	Lymph node[UBERON:UBERON_0000029]

Donor Information

Age	56
Age Category	Adult
Sex	Male
Race	caucasian

Disease Information

Disease	Melanoma
Lineage	Skin
Subtype	Melanoma
OncoTree Code	MEL

DepMap Information

Source Type	ATCC
Source ID	ACH-000014_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TERT	c.1-124C>T (c.228C>T) (C228T)	Unspecified	In promoter	from parent cell line Hep-G2
MutationSimple	BRAF	p.Val600Glu (c.1799T>A)	Unspecified	-	PubMed=26214590

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X

CSF1PO

11

D13S317

11,12

D16S539

11,12

D18S51

13,17

D19S433

14,14.2

D21S11

30

D2S1338

17,24

D3S1358

16,17

D5S818

12

D7S820

10

D8S1179

13,14

FGA

23,24

Penta D

13

Penta E

7,10

TH01

8,9.3

TPOX

8,11

vWA

16,18

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Characterization of two melanoma cell lines resistant to BRAF/MEK inhibitors (vemurafenib and cobimetinib).

Nowak D.

Cell Commun. Signal. 22:410.1-410.21(2024).

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

Involvement of overexpressed wild-type BRAF in the growth of malignant melanoma cell lines.

Yasui K., Misawa-Furihata A., Kawakami Y., Inazawa J.

Oncogene 23:8796-8804(2004).

Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.

Wolf E., Gabius H.-J.

J. Cancer Res. Clin. Oncol. 127:375-386(2001).

Expression of hyaluronidase by tumor cells induces angiogenesis in vivo.

Markowitz S.D., Willson J.K.V., Sy M.-S.

Proc. Natl. Acad. Sci. U.S.A. 93:7832-7837(1996).

Polymorphic enzyme analysis of cultured human tumor cell lines.";

Dracopoli N.C., Fogh J.

J. Natl. Cancer Inst. 70:469-476(1983).

Human tumor lines for cancer research.";

Fogh J.

Cancer Invest. 4:157-184(1986).

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Cancer Res. 48:589-601(1988).

Biological properties of human melanoma cells in culture.";

Madin S.H.

In Vitro 15:342-350(1979).

Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces.

Sherwin S.A., Sliski A.H., Todaro G.J.

Proc. Natl. Acad. Sci. U.S.A. 76:1288-1292(1979).

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

Fogh J., Fogh J.M., Orfeo T.

J. Natl. Cancer Inst. 59:221-226(1977).

Nerve growth factor receptors on human melanoma cells in culture.";

Fabricant R.N., De Larco J.E., Todaro G.J.

Proc. Natl. Acad. Sci. U.S.A. 74:565-569(1977).