HuT 78Homo sapiens (Human)Cancer cell line

Also known as: NCI-H78, HUT78, Hut78, HuT78, HUT-78, HuT-78, HUT 78, Hut 78

🤖 AI SummaryBased on 13 publications

HUT 78

Quick Overview

Human T-cell leukemia/lymphoma cell line with potential for studying immune disorders and cancer therapies.

Detailed Summary

HUT 78 is a human T-cell leukemia/lymphoma cell line derived from a patient with Sezary syndrome. It is characterized by its expression of T-cell markers and its ability to produce and respond to T-cell growth factor (TCGF). This cell line has been instrumental in the study of T-cell malignancies and has contributed to the identification of retroviruses such as HTLV-1. HUT 78 is used in research to understand the biology of T-cell lymphomas and leukemias, including their genetic alterations and responses to therapeutic agents. The cell line is also notable for its role in the development of diagnostic assays for HTLV-1 infection.

Research Applications

Study of T-cell malignanciesIdentification of retroviruses (e.g., HTLV-1)Development of diagnostic assays for HTLV-1Investigation of T-cell growth factor (TCGF) interactionsAnalysis of genetic alterations in T-cell lymphomas

Key Characteristics

Expresses T-cell markersProduces and responds to TCGFUsed in retrovirus researchRelevant to HTLV-1 infection studies

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0337
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age	53
Age Category	Adult
Sex	Male
Race	caucasian

Disease Information

Disease	Sezary syndrome
Lineage	Lymphoid
Subtype	Sezary Syndrome
OncoTree Code	SS

DepMap Information

Source Type	ATCC
Source ID	ACH-000509_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg196Ter (c.586C>T)	Unspecified	-	PubMed=25275298
MutationSimple	NRAS	p.Gln61Lys (c.181C>A)	Unspecified	Acquired during resistance selection process	PubMed=26214590
Gene deletion	MPDZ	-	Homozygous	-	PubMed=20215515

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

11,12

D13S317

8,12

D16S539

11,12

D18S51

D19S433

D21S11

D2S1338

20,25

D3S1358

15,16

D5S818

11,12

D7S820

D8S1179

12,14

FGA

21,25

Penta D

Penta E

13,15

TH01

8,9

TPOX

8,9

vWA

14,15

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

High-throughput RNA sequencing-based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project.

O'Grady T., Baddoo M., Fewell C., Renne R., Flemington E.K.

J. Virol. 89:713-729(2015).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Mutations of the BRAF gene in human cancer.";

Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.

Nature 417:949-954(2002).

DOI PubMed

Short tandem repeat profiling provides an international reference standard for human cell lines.

Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).

DOI PubMed PMC

Chromosomal imbalances in adult T-cell leukemia revealed by comparative genomic hybridization: gains at 14q32 and 2p16-22 in cell lines.

Yamada Y., Isobe M., Seto M., Nakamura Y., Inazawa J.

J. Hum. Genet. 44:357-363(1999).

DOI PubMed

Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.

Aizawa Y., Ueda R., Seto M.

Jpn. J. Cancer Res. 89:712-718(1998).

DOI PubMed PMC

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

DOI PubMed

T-cell lymphoma cell lines (HUT102 and HUT78) established at the National Cancer Institute: history and importance to understanding the biology, clinical features, and therapy of cutaneous T-cell lymphomas (CTCL) and adult T-cell leukemia-lymphomas (ATLL).

Bunn P.A. Jr., Foss F.M.

J. Cell. Biochem. Suppl. 24:12-23(1996).

DOI PubMed

NCI series of cell lines: an historical perspective.";

Gazdar A.F., Minna J.D.

J. Cell. Biochem. 63 Suppl. 24:1-11(1996).

DOI PubMed

Interferon system defects in malignant T-cells.";

Xu B., Einhorn S.

Leukemia 8:425-434(1994).

PubMed

Macaques immunized with HLA-DR are protected from challenge with simian immunodeficiency virus.

Mann D.L., Henderson L.E., Benveniste R.E.

J. Virol. 69:3117-3124(1995).

DOI PubMed PMC

Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor.

Gootenberg J.E., Ruscetti F.W., Mier J.W., Gazdar A.F., Gallo R.C.

J. Exp. Med. 154:1403-1418(1981).

DOI PubMed PMC

Differential expression of the amv gene in human hematopoietic cells.

Aaronson S.A., Wong-Staal F.

Proc. Natl. Acad. Sci. U.S.A. 79:2194-2198(1982).

DOI PubMed PMC

Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas.

Schechter G.P., Guccion J.G.

Blood 55:409-417(1980).

DOI PubMed

T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III.

Popovic M., Read-Connole E.L., Gallo R.C.

Lancet 324:1472-1473(1984).

DOI PubMed

Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:537-548(1985).

DOI PubMed

Constitutive production of B cell differentiation factor-like activity by human T and B cell lines.

Kanowith-Klein S., Saxon A., Uittenbogaart C.H.

Eur. J. Immunol. 17:593-598(1987).

DOI PubMed

Isoenzyme studies in human leukemia-lymphoma cell lines -- III. Beta-hexosaminidase (E.C. 3.2.1.30).

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:549-559(1985).

DOI PubMed

Isoenzyme studies in human leukemia-lymphoma cell lines -- 1. carboxylic esterase.

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:209-229(1985).

DOI PubMed

Origin of the HIV-susceptible human CD4+ cell line H9.";

Read-Connole E.L., Gallo R.C., Gazdar A.F.

AIDS Res. Hum. Retroviruses 5:253-255(1989).

DOI PubMed

The untold story of HUT78.";

Rubinstein E.

Science 248:1499-1507(1990).

DOI PubMed

Frequent mutations in the p53 tumor suppressor gene in human leukemia T-cell lines.

Cheng J., Haas M.

Mol. Cell. Biol. 10:5502-5509(1990).

DOI PubMed PMC

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI

Web Resources

thermofisher.com nibsc.org

Anecdotal Information

For a number of years it was not known that H9 used by Gallo's lab to isolate HIV-1 (HTLV-III) was in fact a clone of Hut 78 from Minna's lab (PubMed=2567177). This created a controversy between the labs of Minna and Gallo (PubMed=2193399)