Ku812Homo sapiens (Human)Cancer cell line

Also known as: KU 812, KU.812, KU-812, KU812

🤖 AI SummaryBased on 14 publications

KU812

Quick Overview

Human basophil progenitor cell line with BCR-ABL fusion for leukemia research.

Detailed Summary

KU812 is a human basophil progenitor cell line derived from a patient with chronic myeloid leukemia (CML). It expresses the BCR-ABL fusion gene, a hallmark of CML, which results from the Philadelphia chromosome translocation. This cell line is widely used in research to study the molecular mechanisms of CML and to test therapeutic strategies targeting the BCR-ABL tyrosine kinase. KU812 is also utilized in investigations of cell proliferation, apoptosis, and drug resistance. The cell line has been characterized in multiple studies for its genetic and phenotypic stability, making it a reliable model for studying leukemogenesis and evaluating potential anticancer agents.

Research Applications

Leukemia researchBCR-ABL fusion gene studiesTyrosine kinase inhibitor testingCell proliferation and apoptosis studiesDrug resistance mechanisms

Key Characteristics

BCR-ABL fusion gene expressionPhenotypic stabilityRelevance to CML pathogenesis

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0379
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age	38
Age Category	Adult
Sex	Male
Race	asian
Subtype Features	BCR-ABL1 positive

Disease Information

Disease	Chronic myeloid leukemia
Lineage	Myeloid
Subtype	Chronic Myeloid Leukemia, BCR-ABL1+
OncoTree Code	CMLBCRABL1

DepMap Information

Source Type	ATCC
Source ID	ACH-000074_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Lys132Arg (c.395A>G)	Unspecified	-	PubMed=34732238
Gene fusion	ABL1	BCR-ABL1, BCR-ABL	-	BCR exon 1 fused to ABL1 exon 2	PubMed=10576511, PubMed=10071072, PubMed=8751477

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

12,13

D13S317

D16S539

D18S51

D19S433

13,14

D21S11

30,31.2

D2S1338

D3S1358

D5S818

D7S820

D8S1179

11,15

FGA

22,24

Penta D

Penta E

5,19

TH01

TPOX

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

The LL-100 panel: 100 cell lines for blood cancer studies.";

MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.

Sci. Rep. 9:8218-8218(2019).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

DOI PubMed PMC

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOI PubMed

Mast cell and basophil cell lines: a compendium.";

Passante E.

Methods Mol. Biol. 1192:101-113(2014).

DOI PubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

Genomic amplification of BCR/ABL1 and a region downstream of ABL1 in chronic myeloid leukaemia: a FISH mapping study of CML patients and cell lines.

Virgili A., Nacheva E.

Mol. Cytogenet. 3:15.1-15.12(2010).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

Leukemia 20:471-476(2006).

DOI PubMed

Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.

Fioretos T.

Leukemia 19:1042-1050(2005).

DOI PubMed

Short tandem repeat profiling provides an international reference standard for human cell lines.

Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).

DOI PubMed PMC

In vitro cytotoxic effects of a tyrosine kinase inhibitor STI571 in combination with commonly used antileukemic agents.

Furukawa Y.

Blood 97:1999-2007(2001).

DOI PubMed

ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.";

Uphoff C.C., Habig S., Fombonne S., Matsuo Y., Drexler H.G.

Leuk. Res. 23:1055-1060(1999).

DOI PubMed

Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.

Drexler H.G., MacLeod R.A.F., Uphoff C.C.

Leuk. Res. 23:207-215(1999).

DOI PubMed

Rare alteration of genomic structure or expression of the DPC4 gene in myelogenous leukemias.

Taniwaki M., Kashima K., Misawa S.-i.

Acta Haematol. 99:187-190(1998).

DOI PubMed

Frameshift mutations of the hMSH6 gene in human leukemia cell lines.

Hirai H.

Jpn. J. Cancer Res. 89:33-39(1998).

DOI PubMed PMC

Screening the p53 status of human cell lines using a yeast functional assay.

Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.

Mol. Carcinog. 19:243-253(1997).

DOI PubMed

A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.

Kishi K.

Leuk. Res. 9:381-390(1985).

DOI PubMed

Ph positive CML cell lines.";

Keating A.

Baillieres Clin. Haematol. 1:1021-1029(1987).

DOI PubMed

KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.

Beuzard Y., Kishi K., Breton-Gorius J., Vainchenker W.

Blood 73:2003-2013(1989).

DOI PubMed

Identification of cell lines with variable numbers of tandem repeat (VNTR) amplified by polymerase chain reaction.

Matsuo Y., Okochi A., Ariyasu T., Iimura E., Ohno T.

Tissue Cult. Res. Commun. 15:211-219(1996).

DOI

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI