M059KHomo sapiens (Human)Cancer cell line

🤖 AI SummaryBased on 8 publications

Quick Overview

Human glioblastoma cell line with distinct radiosensitivity and drug response profiles.

Detailed Summary

M059K is a human glioblastoma cell line derived from a single tumor specimen, showing significant differences in radiosensitivity and drug response compared to its isogenic counterpart M059J. It exhibits a higher survival fraction after radiation exposure (SF2 = 0.64) and is less sensitive to chemotherapeutic agents like bleomycin, BCNU, and nitrogen mustard. The cell line is aneuploid and has been used to study DNA repair mechanisms, radiation sensitivity, and tumor heterogeneity. M059K is part of the Cancer Cell Line Encyclopedia (CCLE) and has been characterized for its genetic and molecular profiles, making it a valuable model for cancer research and drug development.

Research Applications

Radiosensitivity studiesDrug response profilingDNA repair mechanism analysisTumor heterogeneity investigationCancer immunotherapy research

Key Characteristics

AneuploidHigh SF2 value (0.64)Less sensitive to chemotherapeutic agentsPart of CCLEUsed for molecular profiling

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0401
Species	Homo sapiens (Human)
Tissue Source	Brain[UBERON:UBERON_0000955]

Donor Information

Age	33
Age Category	Adult
Sex	Male

Disease Information

Disease	Glioblastoma
Lineage	CNS/Brain
Subtype	Glioblastoma
OncoTree Code	GB

DepMap Information

Source Type	ATCC
Source ID	ACH-000152_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Glu286lys (c.856G>A)	Homozygous	-	Unknown, PubMed=11023613
MutationSimple	PTEN	p.Pro204fs*17 (c.610delC)	Homozygous	-	Unknown

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

10,12

D13S317

D16S539

10,12

D18S51

15,19

D19S433

D21S11

30,31.2

D2S1338

20,24

D3S1358

14,15

D5S818

11,12

D7S820

D8S1179

FGA

20,26

Penta D

12,13

Penta E

7,15

TH01

9.3

TPOX

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOI PubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

MAP kinase-interacting kinase 1 regulates SMAD2-dependent TGF-beta signaling pathway in human glioblastoma.

Moncayo G., Hemmings B.A.

Cancer Res. 71:2392-2402(2011).

DOI PubMed

Profiling of UV-induced ATM/ATR signaling pathways.";

Polakiewicz R.D., Comb M.J.

Proc. Natl. Acad. Sci. U.S.A. 104:19855-19860(2007).

DOI PubMed PMC

Detection of ATM gene mutation in human glioma cell line M059J by a rapid frameshift/stop codon assay in yeast.

Igarashi T., Chessa L., Delia D., Teraoka H., Mizutani S.

Radiat. Res. 158:195-201(2002).

DOI PubMed

Human TP53 from the malignant glioma-derived cell lines M059J and M059K has a cancer-associated mutation in exon 8.

Anderson C.W., Allalunis-Turner M.J.

Radiat. Res. 154:473-476(2000).

DOI PubMed

Complementation of the radiosensitive M059J cell line.";

Hoppe B.S., Jensen R.B., Kirchgessner C.U.

Radiat. Res. 153:125-130(2000).

DOI PubMed

Isolation of two cell lines from a human malignant glioma specimen differing in sensitivity to radiation and chemotherapeutic drugs.

Mirzayans R.

Radiat. Res. 134:349-354(1993).

DOI PubMed

Radiation-induced DNA damage and repair in cells of a radiosensitive human malignant glioma cell line.

Day R.S. 3rd

Radiat. Res. 144:288-293(1995).

DOI PubMed