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MG-63Homo sapiens (Human)Cancer cell line

Also known as: MG63, M-G63, MGG3 (In figure 4.)

🤖 AI SummaryBased on 13 publications

Quick Overview

MG-63 is a human osteosarcoma cell line used in cancer research.

Detailed Summary

MG-63 is a human osteosarcoma cell line derived from a bone tumor. It is widely used in research to study the biology of osteosarcoma, including metastasis, drug response, and genetic alterations. The cell line has been characterized for its ability to form tumors in vivo and differentiate into various cell types. Research on MG-63 has contributed to understanding the role of enhancer elements in metastasis and the identification of potential therapeutic targets. Additionally, it has been used to investigate the effects of microgravity on osteoblastic cells and to study the molecular mechanisms underlying cancer progression.

Research Applications

Metastasis researchDrug response studiesGenetic and epigenetic alterationsTumor formation in vivoDifferentiation capacityMicrogravity effects on osteoblasts

Key Characteristics

High metastatic potentialAbility to form tumors in vivoDifferentiation into multiple cell lineagesUsed in studies of enhancer elements and metastasisRelevance to osteosarcoma biology
Generated on 6/15/2025

Basic Information

Database IDCVCL_0426
SpeciesHomo sapiens (Human)
Tissue SourceBone, left femur[UBERON:UBERON_0000981]

Donor Information

Age14
Age CategoryPediatric
SexMale
Racecaucasian

Disease Information

DiseaseOsteosarcoma
LineageBone
SubtypeOsteosarcoma
OncoTree CodeOS

DepMap Information

Source TypeATCC
Source IDACH-000359_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
MutationNone reportedTP53---PubMed=19787792
Gene deletionCDKN2A-HomozygousPossiblePubMed=26870271

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X,Y
CSF1PO
10,12
D13S317
11
D16S539
11
D18S51
16
D19S433
13,14
D21S11
30
D2S1338
17,24
D3S1358
15
D5S818
11,12
D7S820
10
D8S1179
13
FGA
21,25
Penta D
9,13
Penta E
11,12
SE33
20
TH01
9.3
TPOX
8,11
vWA
16,19
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines

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Publications

Establishment and characterization of single and triple-agent resistant osteosarcoma cell lines.

Low K., Hills F., Roberts H.C., Stordal B.

Adv. Biol. (Weinh.) 7:e2200194.1-e2200194.13(2023).

DOIPubMed

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOIPubMedPMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOIPubMedPMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOIPubMedPMC

Positively selected enhancer elements endow osteosarcoma cells with metastatic competence.

Versteeg H.H., Stamatoyannopoulos J.A., Khanna C., Scacheri P.C.

Nat. Med. 24:176-185(2018).

DOIPubMedPMC

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

DOIPubMedPMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOIPubMedPMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOIPubMedPMC

Characterization of the metastatic phenotype of a panel of established osteosarcoma cells.

Luu H.H., Meltzer P.S., Khanna C.

Oncotarget 6:29469-29481(2015).

DOIPubMedPMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOIPubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOIPubMed

Metabolic markers of MG-63 osteosarcoma cell line response to doxorubicin and methotrexate treatment: comparison to cisplatin.

Lamego I., Duarte I.F., Marques M.P.M., Gil A.M.

J. Proteome Res. 13:6033-6045(2014).

DOIPubMed

Rac1 GTPase silencing counteracts microgravity-induced effects on osteoblastic cells.

Demets R., Colige A., Vico L.

FASEB J. 28:4077-4087(2014).

DOIPubMedPMC

Integrative analysis reveals relationships of genetic and epigenetic alterations in osteosarcoma.

Myklebost O., Meza-Zepeda L.A.

PLoS ONE 7:E48262-E48262(2012).

DOIPubMedPMC

Genetic and molecular characterization of the human osteosarcoma 3AB-OS cancer stem cell line: a possible model for studying osteosarcoma origin and stemness.

Tesoriere G., Vento R., Russo A.

J. Cell. Physiol. 228:1189-1201(2013).

DOIPubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOIPubMedPMC

Functional characterization of osteosarcoma cell lines provides representative models to study the human disease.

Hogendoorn P.C.W., Llombart-Bosch A., Cleton-Jansen A.-M.

Lab. Invest. 91:1195-1205(2011).

DOIPubMed

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOIPubMedPMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOIPubMedPMC

Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.

Buerger H., Aigner T., Gabbert H.E., Poremba C.

Genes Chromosomes Cancer 49:40-51(2010).

DOIPubMed

Establishment and characterization of a new highly metastatic human osteosarcoma cell line.

Chen J., Jin X.-Q., Haydon R.C., He T.-C., Luu H.H.

Clin. Exp. Metastasis 26:599-610(2009).

DOIPubMed

Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment.

Messina C., Pirozzi G., Tirino V., Tesoriere G., Vento R.

J. Cell. Physiol. 219:301-313(2009).

DOIPubMed

Identification of cryptic microaberrations in osteosarcoma by high-definition oligonucleotide array comparative genomic hybridization.

Squire J.A., Zielenska M.

Cancer Genet. Cytogenet. 179:52-61(2007).

DOIPubMed

Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts.

Milz S.

Anticancer Res. 24:3743-3748(2004).

PubMed

Chromosomal alterations in osteosarcoma cell lines revealed by comparative genomic hybridization and multicolor karyotyping.

Poremba C.

Cancer Genet. Cytogenet. 140:145-152(2003).

DOIPubMed

Gene expression related to the differentiation of osteoblastic cells is altered by microgravity.

Carmeliet G., Nys G., Stockmans I., Bouillon R.

Bone 22:139S-143S(1998).

DOIPubMed

Microgravity reduces the differentiation of human osteoblastic MG-63 cells.

Carmeliet G., Nys G., Bouillon R.

J. Bone Miner. Res. 12:786-794(1997).

DOIPubMed

Immunostaining of the p30/32MIC2 antigen and molecular detection of EWS rearrangements for the diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumor.

Lollini P.-L., Picci P., Bertoni F., Baldini N.

Hum. Pathol. 27:408-416(1996).

DOIPubMed

Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype?

Clover J., Gowen M.

Bone 15:585-591(1994).

DOIPubMed

Rearrangement of the p53 gene in human osteogenic sarcomas.";

Masuda H., Miller C., Koeffler H.P., Battifora H.A., Cline M.J.

Proc. Natl. Acad. Sci. U.S.A. 84:7716-7719(1987).

DOIPubMedPMC

p53 functions as a cell cycle control protein in osteosarcomas.";

Bressac B., Ozturk M., Baker S.J., Vogelstein B., Friend S.H.

Mol. Cell. Biol. 10:5772-5781(1990).

DOIPubMedPMC

Effects of combining transforming growth factor beta and 1,25-dihydroxyvitamin D3 on differentiation of a human osteosarcoma (MG-63).

Johnson T.L., Leach R.J., Boyan B.D.

J. Biol. Chem. 267:8943-8949(1992).

DOIPubMed

Human interferon: mass production in a newly established cell line, MG-63.

Georgiades J.A., De Somer P.

Antimicrob. Agents Chemother. 12:11-15(1977).

DOIPubMedPMC

In vitro cultivation of human tumor tissues. II. Morphological and virological characterization of three cell lines.

Heremans H., Billiau A., Cassiman J.-J., Mulier J.C., De Somer P.

Oncology 35:246-252(1978).

DOIPubMed

Web Resources

thermofisher.comtcpaportal.org

Anecdotal Information

  • Have been flown in space on Foton-10 and Foton-M3 to study if differentiation is altered in microgravity (PubMed=9144345; PubMed=9600771; PubMed=24903274)