NCI-H295RHomo sapiens (Human)Cancer cell line

Also known as: H295R-S1, H-295R, H295R, NCIH295R, NCI H295R

🤖 AI SummaryBased on 4 publications

Quick Overview

Human adrenocortical carcinoma cell line for aldosterone and steroid research

Detailed Summary

The NCI-H295R cell line is a human adrenocortical carcinoma cell line derived from an adrenal tumor. It is widely used in research for studying aldosterone production and steroidogenesis. This cell line exhibits significant variability in aldosterone production capacity compared to other adrenocortical cell lines. NCI-H295R cells are capable of producing aldosterone and respond to angiotensin II (Ang II) stimulation, making them valuable for investigating the regulation of aldosterone synthesis. They also express the type 1 angiotensin II receptor (AT1 receptor) and show responsiveness to ACTH and other hormonal regulators. The cell line has been used to study the molecular mechanisms of adrenal steroidogenesis and the role of various enzymes and receptors in steroid hormone production. NCI-H295R cells are particularly useful for understanding the regulation of aldosterone biosynthesis and the effects of hormonal stimuli on steroid production.

Research Applications

Aldosterone productionSteroidogenesisHormonal regulation of steroid synthesisMolecular mechanisms of adrenal steroidogenesis

Key Characteristics

Capable of producing aldosteroneResponds to angiotensin II (Ang II) stimulationExpresses type 1 angiotensin II receptor (AT1 receptor)Responsive to ACTH and other hormonal regulatorsUsed for studying adrenal steroidogenesis

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0458
Species	Homo sapiens (Human)
Tissue Source	Adrenal gland[UBERON:UBERON_0002369]

Donor Information

Age	48
Age Category	Adult
Sex	Female

Disease Information

Disease	Adrenocortical carcinoma
Lineage	Adrenal Gland
Subtype	Adrenocortical Carcinoma
OncoTree Code	ACC

DepMap Information

Source Type	ATCC
Source ID	ACH-001598_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	CTNNB1	p.Ser45Pro (c.133T>C)	Unspecified	-	PubMed=27698927

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

10,12

D13S317

D16S539

D18S51

D19S433

D21S11

32.2

D2S1338

D3S1358

15,16

D5S818

D7S820

9,12

D8S1179

FGA

19.2,24

Penta D

Penta E

5,12

TH01

9.3

TPOX

vWA

17,18

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

An update on adrenocortical cell lines of human origin.";

Hantel C.

Endocrine 77:432-437(2022).

DOI PubMed PMC

A comprehensive investigation of steroidogenic signaling in classical and new experimental cell models of adrenocortical carcinoma.

Tiberio G.A.M., Bornstein S.R., Berruti A., Hantel C.

Cells 11:1439.1-1439.17(2022).

DOI PubMed PMC

Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to steroidogenic stimuli.

Mukherjee N.

Cells 10:2376.1-2376.15(2021).

DOI PubMed PMC

Cytotoxic effect of progesterone, tamoxifen and their combination in experimental cell models of human adrenocortical cancer.

Poliani P.L., Tiberio G.A.M., Memo M., Sigala S., Berruti A.

Front. Endocrinol. 12:669426.1-669426.13(2021).

DOI PubMed PMC

Development of new preclinical models to advance adrenocortical carcinoma research.

Wierman M.E.

Endocr. Relat. Cancer 25:437-451(2018).

DOI PubMed PMC

NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca(2)(+)-mobilization/influx and cortisol secretion.

Nishi H., Arai H., Momiyama T.

PLoS ONE 8:E71022-E71022(2013).

DOI PubMed PMC

Comparison of aldosterone production among human adrenocortical cell lines.

Wang T., Rowland J.G., Parmar J., Nesterova M., Seki T., Rainey W.E.

Horm. Metab. Res. 44:245-250(2012).

DOI PubMed PMC

Human adrenocortical carcinoma cell lines.";

Wang T., Rainey W.E.

Mol. Cell. Endocrinol. 351:58-65(2012).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Human adrenal corticocarcinoma NCI-H295R cells produce more androgens than NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase type 2 and 17,20 lyase activities.

Fluck C.E.

J. Endocrinol. 195:459-472(2007).

DOI PubMed

Adrenocortical cell lines.";

Rainey W.E., Saner K., Schimmer B.P.

Mol. Cell. Endocrinol. 228:23-38(2004).

DOI PubMed

Web Resources

en.wikipedia.org