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TF-1Homo sapiens (Human)Cancer cell line

Also known as: MFD-1, TF1

🤖 AI SummaryBased on 12 publications

Quick Overview

TF-1 is a human erythroleukemia cell line dependent on GM-CSF, IL-3, or EPO for growth.

Detailed Summary

TF-1 is a human erythroleukemia cell line that requires granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), or erythropoietin (EPO) for proliferation. It exhibits characteristics of immature erythroid cells and can differentiate into more mature erythroid cells or macrophage-like cells under specific conditions. TF-1 has been used to study the mechanisms of hematopoietic growth factor signaling and the role of specific receptors in cell growth and differentiation. The cell line is also utilized in research on the effects of various inducers on cell differentiation and in the analysis of gene expression patterns associated with erythroid lineage commitment.

Research Applications

Hematopoietic growth factor signalingErythroid differentiationGene expression analysisSignal transduction mechanisms

Key Characteristics

Dependent on GM-CSF, IL-3, or EPO for growthImmature erythroid lineageCapable of differentiation into macrophage-like cellsUsed in studies of hematopoietic transformation

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0559
Species	Homo sapiens (Human)
Tissue Source	Bone marrow[UBERON:UBERON_0002371]

Donor Information

Age	35
Age Category	Adult
Sex	Male
Race	asian

Disease Information

Disease	Acute erythroid leukemia
Lineage	Myeloid
Subtype	Acute Myeloid Leukemia
OncoTree Code	AML

DepMap Information

Source Type	ATCC
Source ID	ACH-000387_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Ile251Thrfs*94 (c.752delT)	Unspecified	-	from parent cell line TF-1
MutationSimple	NRAS	p.Gln61Pro (c.182A>C)	Heterozygous	-	from parent cell line TF-1
Gene fusion	ABHD12	CBFA2T3-ABHD12	-	-	from parent cell line TF-1

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

13

D13S317

8,9

D16S539

9,12

D18S51

13

D19S433

14,16

D21S11

30

D2S1338

19

D3S1358

15

D5S818

13

D7S820

12

D8S1179

11,15

FGA

18,19

Penta D

10,13

Penta E

5,17

TH01

7,9

TPOX

8

vWA

15,17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

The LL-100 panel: 100 cell lines for blood cancer studies.";

MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.

Sci. Rep. 9:8218-8218(2019).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.

Haferlach T., Shirahige K., Miyano S., Ogawa S.

Nat. Genet. 45:1232-1237(2013).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

Leukemia 20:471-476(2006).

Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.

Wolf E., Gabius H.-J.

J. Cancer Res. Clin. Oncol. 127:375-386(2001).

Biological significance of the expression of HIV-related chemokine coreceptors (CCR5 and CXCR4) and their ligands by human hematopoietic cell lines.

Gaulton G.N., Ratajczak M.Z.

Leukemia 14:1821-1832(2000).

Frameshift mutations of the hMSH6 gene in human leukemia cell lines.

Hirai H.

Jpn. J. Cancer Res. 89:33-39(1998).

Thrombopoietin supports the continuous growth of cytokine-dependent human leukemia cell lines.

Drexler H.G., Zaborski M., Quentmeier H.

Leukemia 11:541-551(1997).

Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin.

Piao Y.-F., Miyazono K., Urabe A., Takaku F.

J. Cell. Physiol. 140:323-334(1989).

Origins and properties of hematopoietic growth factor-dependent cell lines.

Ihle J.N., Askew D.

Int. J. Cell Cloning 7:68-91(1989).

Frequent mutations in the p53 gene in human myeloid leukemia cell lines.

Ishikawa F., Takaku F., Yazaki Y., Hirai H.

Blood 79:2378-2383(1992).

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

Web Resources