TK6Homo sapiens (Human)Transformed cell line

Also known as: H2BT, TK-6

🤖 AI SummaryBased on 9 publications

Quick Overview

Human lymphoblastoid cell line used in genotoxicity studies.

Detailed Summary

TK6 is a human B-lymphoblastoid cell line derived from a lymphoblastoid line, originally isolated from a male with hereditary spherocytosis. It is widely used in genotoxicity testing, particularly in the in vitro micronucleus assay and for studying mutations at the thymidine kinase (TK) locus. The cell line is heterozygous at the TK locus, making it suitable for studying mutations and genetic alterations. TK6 is also used to assess the effects of ionizing radiation and chemical mutagens on DNA damage and repair mechanisms. Research has shown that TK6 cells are sensitive to various mutagens and exhibit distinct responses in terms of micronucleus formation and gene mutation frequencies. The cell line is valuable for understanding the molecular mechanisms of mutagenesis and for evaluating the genotoxic potential of chemicals and radiation.

Research Applications

In vitro micronucleus assayGenotoxicity testingMutation analysis at the thymidine kinase locusDNA damage and repair studiesAssessment of chemical and radiation mutagenicity

Key Characteristics

Heterozygous at the thymidine kinase (TK) locusUsed for studying mutations and genetic alterationsSensitive to various mutagens and ionizing radiationSuitable for evaluating genotoxic potential of chemicals

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0561
Species	Homo sapiens (Human)
Tissue Source	Spleen[UBERON:UBERON_0002106]

Donor Information

Age	30
Age Category	Adult
Sex	Male
Subtype Features	BCR-ABL1

Disease Information

Disease	Hereditary spherocytosis
Lineage	Myeloid
Subtype	Chronic Myeloid Leukemia, BCR-ABL1+
OncoTree Code	CMLBCRABL1

DepMap Information

Source Type	DSMZ
Source ID	ACH-002676_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationNone reported	TP53	-	-	-	PubMed=19787792
MutationSimple	TK1	p.Ala78Argfs*27 (c.231insC)	Heterozygous	-	PubMed=11221843
MutationSimple	SPTA1	p.Ser1163Ala (c.3487T>G)	Homozygous	-	from parent cell line WIL2 NS

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

11,12

D13S317

D16S539

11,12

D18S51

11,16

D19S433

14,16

D21S11

D2S1338

17,25

D3S1358

D5S818

12,13

D7S820

9,11

D8S1179

10,13

FGA

22,24

Penta D

11,12

Penta E

5,7

TH01

8,9.3

TPOX

8,11

vWA

17,20

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Standardized cell sources and recommendations for good cell culture practices in genotoxicity testing.

Gollapudi B.B., Aardema M.J., Tanir J.Y.

Mutat. Res. Genet. Toxicol. Environ. Mutagen. 809:1-15(2016).

DOI PubMed

Whole genome and normalized mRNA sequencing reveal genetic status of TK6, WTK1, and NH32 human B-lymphoblastoid cell lines.

Dobrovolsky V.N.

Mutat. Res. Genet. Toxicol. Environ. Mutagen. 795:60-69(2016).

DOI PubMed

Different responses to 5-fluoraouracil in mutagenicity and gene expression between two human lymphoblastoid cell lines with or without TP53 mutation.

Oka H., Ouchida M., Kondo T., Morita F., Shimizu K.

Acta Med. Okayama 66:119-129(2012).

DOI PubMed

Difference in susceptibility to morphological changes in the nucleus to aneugens between p53-competent and p53-abrogated lymphoblastoid cell lines (TK6 and NH32 cells) in the in vitro micronucleus assay.

Hashimoto K., Nakajima Y., Uematsu R., Chatani F.

Mutagenesis 27:287-293(2012).

DOI PubMed

The utility of DNA microarrays for characterizing genotoxicity.";

Newton R.K., Aardema M.J., Aubrecht J.

Environ. Health Perspect. 112:420-422(2004).

DOI PubMed PMC

Different mechanisms of radiation-induced loss of heterozygosity in two human lymphoid cell lines from a single donor.

Kronenberg A.

Cancer Res. 61:1129-1137(2001).

PubMed

Radiation-induced mutations at the autosomal thymidine kinase locus are not elevated in p53-null cells.

Chuang Y.-Y.E., Chen Q., Brown J.P., Sedivy J.M., Liber H.L.

Cancer Res. 59:3073-3076(1999).

PubMed

Single and coincident intragenic mutations attributable to gene conversion in a human cell line.

Giver C.R., Grosovsky A.J.

Genetics 146:1429-1439(1997).

DOI PubMed PMC

DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.

Olive P.L.

Radiat. Res. 134:307-315(1993).

DOI PubMed

Mutational spectrum of X-ray induced TK- human cell mutants.";

Grosovsky A.J.

Carcinogenesis 16:267-275(1995).

DOI PubMed

Absence of radiation-induced G1 arrest in two closely related human lymphoblast cell lines that differ in p53 status.

Little J.B., Nagasawa H., Keng P.C., Yu Y.-J., Li C.-Y.

J. Biol. Chem. 270:11033-11036(1995).

DOI PubMed

A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test.

Zhang L.-S., Honma M., Hayashi M., Suzuki T., Matsuoka A., Sofuni T.

Mutat. Res. 347:105-115(1995).

DOI PubMed

Somatic mutations at a heterozygous autosomal locus in human cells occur more frequently by allele loss than by intragenic structural alterations.

Yandell D.W., Dryja T.P., Little J.B.

Somat. Cell Mol. Genet. 12:255-263(1986).

DOI PubMed

Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay.

Skopek T.R., Liber H.L., Penman B.W., Thilly W.G.

Biochem. Biophys. Res. Commun. 84:411-416(1978).

DOI PubMed