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MDA-MB-435SHomo sapiens (Human)Cancer cell line

Also known as: BrCL15, MDAMB435S, MDA-MB-435-S, MDA-MB-435 S, MDA-MB-435s

🤖 AI SummaryBased on 14 publications

Quick Overview

Human breast cancer cell line with melanocytic origin, used in cancer research.

Detailed Summary

MDA-MB-435S is a human breast cancer cell line that has been identified as having a melanocytic origin rather than a typical breast cancer lineage. This cell line is widely used in cancer research, particularly in studies involving breast cancer and melanoma. It exhibits characteristics of both epithelial and melanocytic cells, including the expression of markers such as S100 and melan A. The cell line has been shown to have mutations in the p53 gene and other genetic alterations, which may contribute to its tumorigenic properties. Research on MDA-MB-435S has provided insights into the molecular mechanisms of cancer progression and has been utilized in studies related to drug sensitivity and genetic diversity in cancer cells.

Research Applications

Cancer researchBreast cancer studiesMelanoma researchGenetic mutation analysisDrug sensitivity testing

Key Characteristics

Melanocytic originExpression of S100 and melan Ap53 gene mutationsGenetic heterogeneityUsed in drug screening

Generated on 6/15/2025

Basic Information

Database ID	CVCL_0622
Species	Homo sapiens (Human)
Tissue Source	Right buttock, hypodermis[UBERON:UBERON_0002072]

Donor Information

Age	31
Age Category	Adult
Sex	Unknown
Race	caucasian

Disease Information

Disease	Amelanotic melanoma
Lineage	Skin
Subtype	Melanoma
OncoTree Code	MEL

DepMap Information

Source Type	ATCC
Source ID	ACH-000884_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Gly266Glu (c.797G>A)	Unspecified	-	PubMed=21173094
MutationSimple	EP300	p.Glu1013Gly (c.3038A>G)	Hemizygous	-	PubMed=10700188
MutationSimple	EP300	p.Leu827Pro (c.2480T>C)	Hemizygous	-	PubMed=10700188
MutationSimple	CDKN2A	c.455insCdel26	Heterozygous	-	from parent cell line OCM-1
MutationSimple	CDKN2A	c.150+2T>C (IVS1+2T>C)	Heterozygous	Splice donor mutation	from parent cell line OCM-1
MutationSimple	BRAF	p.Val600Glu (c.1799T>A)	Unspecified	-	PubMed=26214590

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X

CSF1PO

11

D12S391

18,19

D13S317

12

D16S539

13

D18S51

13

D19S433

14

D1S1656

12

D21S11

30

D2S1338

19,24

D3S1358

14

D5S818

11,12

D6S1043

11

D7S820

8

D8S1179

13

FGA

21

Penta D

9,11

Penta E

10,12

TH01

6,7

TPOX

8,11

vWA

16,18

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.

Capes-Davis A.

Int. J. Cancer 142:561-572(2018).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation-specific miRNAs.

Martens J.W.M.

Breast Cancer Res. 15:R33.1-R33.17(2013).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

Breast cancer cell lines carry cell line-specific genomic alterations that are distinct from aberrations in breast cancer tissues: comparison of the CGH profiles between cancer cell lines and primary cancer tissues.

Yamamoto S., Oka M., Hirano T., Sasaki K.

BMC Cancer 10:15.1-15.10(2010).

Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines.

den Bakker M.A., Foekens J.A., Martens J.W.M., Schutte M.

Breast Cancer Res. Treat. 121:53-64(2010).

Thirteen new p53 gene mutants identified among 41 human breast cancer cell lines.

Wasielewski M., Elstrodt F., Klijn J.G.M., Berns E.M.J.J., Schutte M.

Breast Cancer Res. Treat. 99:97-101(2006).

BRCA1 mutation analysis of 41 human breast cancer cell lines reveals three new deleterious mutants.

van den Ouweland A.M.W., Merajver S.D., Ethier S.P., Schutte M.

Cancer Res. 66:41-45(2006).

Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.

Edwards P.A.W., Caldas C.

Carcinogenesis 26:923-930(2005).

Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.

Santibanez-Koref M.F., Schlag P.M., Scherneck S.

Genes Chromosomes Cancer 37:29-35(2003).

Further evidence to support the melanocytic origin of MDA-MB-435.";

Fox J.C.

Mol. Pathol. 55:294-299(2002).

Mutations truncating the EP300 acetylase in human cancers.";

Delhanty J.D.A., Ponder B.A.J., Kouzarides T., Caldas C.

Nat. Genet. 24:300-303(2000).

E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells.

Lochner D., Birchmeier W.

J. Cell Biol. 113:173-185(1991).

Cell lines from human breast.";

Leibovitz A.

(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York; USA (1994).

Web Resources

atcc.org atcc.org iclac.org