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MOLT-3Homo sapiens (Human)Cancer cell line

Also known as: Molt3, MOLT3, Molt 3, MOLT 3, Molt-3

🤖 AI SummaryBased on 14 publications

Quick Overview

Human T-cell leukemia cell line with known genetic alterations and drug resistance profiles.

Detailed Summary

MOLT-3 is a human T-cell leukemia cell line derived from a patient with acute lymphoblastic leukemia. It is characterized by its T-cell lineage and has been extensively studied for its genetic alterations, including mutations in the NOTCH1 gene and microsatellite instability. This cell line is commonly used in research on T-cell malignancies, drug resistance mechanisms, and the molecular pathways involved in leukemia progression. MOLT-3 has been utilized in studies investigating the effects of various chemotherapeutic agents and targeted therapies, making it a valuable model for understanding the biology of T-cell leukemias and developing new treatment strategies.

Research Applications

Genetic alterations in T-cell malignanciesDrug resistance mechanismsMolecular pathways in leukemia progressionChemotherapeutic agent effectsTargeted therapy development

Key Characteristics

T-cell lineageMutations in NOTCH1 geneMicrosatellite instabilityDrug resistance profiles
Generated on 6/15/2025

Basic Information

Database IDCVCL_0624
SpeciesHomo sapiens (Human)
Tissue SourcePeripheral blood[UBERON:UBERON_0000178]

Donor Information

Age19
Age CategoryAdult
SexMale
Subtype FeaturesTAL1

Disease Information

DiseasePrecursor T-cell acute lymphoblastic leukemia
LineageLymphoid
SubtypeAdult T-Cell Leukemia/Lymphoma
OncoTree CodeATLL

DepMap Information

Source TypeNIBRI
Source IDACH-000964_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
MutationSimplePTENp.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA)Heterozygous-Unknown, Unknown, PubMed=25230021
MutationSimpleNRASp.Gln61Lys (c.181C>A)UnspecifiedAcquired during resistance selection processPubMed=26214590
MutationSimpleNOTCH1p.Pro2514Argfs*4 (c.7541_7542delCT)Heterozygous-from parent cell line MOLT-4
MutationSimpleNOTCH1p.Leu1600Pro (c.4799T>C)Unspecified-PubMed=22675565

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X,Y
CSF1PO
11,12
D13S317
11,12,13
D16S539
10,11,13
D18S51
12,13,16,17
D19S433
14,15
D1S1656
15.3,16,16.3
D21S11
28,29,30,31
D2S1338
22,23,24
D3S1358
14,15,16,17
D5S818
12
D7S820
7,8,9
D8S1179
9,13,14,15
FGA
19,21,25
Penta D
8,13
Penta E
14,16
TH01
6,8
TPOX
8
vWA
17
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines

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Publications

Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines.

Lehtio J., Vesterlund M., Jafari R.

Nat. Commun. 13:1691.1-1691.19(2022).

DOIPubMedPMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOIPubMedPMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOIPubMedPMC

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

DOIPubMedPMC

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

DOIPubMedPMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOIPubMed

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOIPubMedPMC

Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes.

Langerak A.W.

Leukemia 21:230-237(2007).

DOIPubMed

Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.

Sanchez-Irizarry C., Blacklow S.C., Look A.T., Aster J.C.

Science 306:269-271(2004).

DOIPubMed

Hydrolytically activated etoposide prodrugs inhibit MDR-1 function and eradicate established MDR-1 multidrug-resistant T-cell leukemia.

Gaedicke G., Wrasidlo W., Reisfeld R.A., Lode H.N.

Blood 102:246-253(2003).

DOIPubMed

N-(4-hydroxyphenyl)retinamide increases ceramide and is cytotoxic to acute lymphoblastic leukemia cell lines, but not to non-malignant lymphocytes.

O'Donnell P.H., Guo W.-X., Reynolds C.P., Maurer B.J.

Leukemia 16:902-910(2002).

DOIPubMed

Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Leuk. Res. 24:255-262(2000).

DOIPubMed

Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: suggestion for classification by immunophenotype and T-cell receptor studies.

Burger R., Hansen-Hagge T.E., Drexler H.G., Gramatzki M.

Leuk. Res. 23:19-27(1999).

DOIPubMed

PTEN gene alterations in lymphoid neoplasms.";

Sakai A., Thieblemont C., Wellmann A., Jaffe E.S., Raffeld M.

Blood 92:3410-3415(1998).

DOIPubMed

Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.

Aizawa Y., Ueda R., Seto M.

Jpn. J. Cancer Res. 89:712-718(1998).

DOIPubMedPMC

The influence of drug-exposure conditions on the development of resistance to methotrexate or ZD1694 in cultured human leukaemia cells.

Jackman A.L.

Int. J. Cancer 66:29-36(1996).

DOIPubMed

Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.

Uppenkamp M.J., Nowrousian M.R., Seeber S., Opalka B.

Br. J. Haematol. 91:350-354(1995).

DOIPubMed

Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia.

Findley H.W. Jr.

Leukemia 9:1159-1161(1995).

PubMed

Growth of human malignant lymphoid cell lines in serum-free medium.";

Uittenbogaart C.H., Cantor Y., Fahey J.L.

In Vitro 19:67-72(1983).

DOIPubMed

Polymorphic enzyme analysis of cultured human tumor cell lines.";

Dracopoli N.C., Fogh J.

J. Natl. Cancer Inst. 70:469-476(1983).

PubMed

Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.

Minowada J., Onuma T., Moore G.E.

J. Natl. Cancer Inst. 49:891-895(1972).

DOIPubMed

Cytogenetic study of human lymphoid T-cell lines derived from lymphocytic leukemia.

Huang C.C., Hou Y., Woods L.K., Moore G.E., Minowada J.

J. Natl. Cancer Inst. 53:655-660(1974).

DOIPubMed

Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:537-548(1985).

DOIPubMed

Evolution of methotrexate resistance of human acute lymphoblastic leukemia cells in vitro.

Holland J.F.

Cancer Res. 45:1815-1822(1985).

PubMed

Human tumor lines for cancer research.";

Fogh J.

Cancer Invest. 4:157-184(1986).

DOIPubMed

Isoenzyme studies in human leukemia-lymphoma cell lines -- III. Beta-hexosaminidase (E.C. 3.2.1.30).

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:549-559(1985).

DOIPubMed

Isoenzyme studies in human leukemia-lymphoma cell lines -- 1. carboxylic esterase.

Drexler H.G., Gaedicke G., Minowada J.

Leuk. Res. 9:209-229(1985).

DOIPubMed

Immunophenotypic and cytogenetic analysis of Molt-3 and Molt-4: human T-lymphoid cell lines with rearrangement of chromosome 7.

Streifel B.J., Kersey J.H.

Blood 72:1755-1760(1988).

DOIPubMed

Detection of T-cell lymphoma-associated antigens on cord blood lymphocytes and phytohemagglutinin-stimulated blasts.

Kaplan J., Peterson W.D. Jr.

Cancer Res. 36:3471-3475(1976).

PubMed

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

Fogh J., Fogh J.M., Orfeo T.

J. Natl. Cancer Inst. 59:221-226(1977).

DOIPubMed

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI

Web Resources

nibsc.org