Hs 944.THomo sapiens (Human)Cancer cell line

Also known as: HS944, Hs 944, HS 944, HS944T, Hs944T, HS 944T, HS-944-T, Hs-944-T, Hs-994

🤖 AI SummaryBased on 6 publications

Quick Overview

Human melanoma cell line with known mutations in NRAS and BRAF, used in cancer research.

Detailed Summary

Hs 944.T is a human melanoma cell line derived from a primary tumor. It is characterized by specific genetic mutations in NRAS and BRAF, which are frequently associated with melanoma progression. This cell line is utilized in research to study the molecular mechanisms of melanoma, including the interactions between RAS signaling pathways and tumor development. The presence of these mutations makes it a valuable model for investigating targeted therapies and understanding the genetic basis of cancer progression. Additionally, Hs 944.T has been used in studies examining the role of PTEN/MMAC1 inactivation in conjunction with BRAF mutations, highlighting its importance in melanoma research.

Research Applications

Cancer researchMelanoma studiesGenetic mutation analysisTargeted therapy development

Key Characteristics

NRAS mutationBRAF mutationPTEN/MMAC1 inactivation

Generated on 6/16/2025

Basic Information

Database ID	CVCL_1040
Species	Homo sapiens (Human)

Donor Information

Age	51
Age Category	Adult
Sex	Male
Race	caucasian

Disease Information

Disease	Cutaneous melanoma
Lineage	Skin
Subtype	Cutaneous Melanoma
OncoTree Code	SKCM

DepMap Information

Source Type	ATCC
Source ID	ACH-000632_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Lys382Asnfs*40 (c.1146delA)	Heterozygous	-	Unknown, Unknown
MutationSimple	TERT	c.242_243CC>TT (-138/-139CC>TT)	Unspecified	In promoter	PubMed=23348503
MutationSimple	PTEN	p.Lys163fs (c.486_487insA)	Heterozygous	-	Unknown
MutationSimple	NRAS	p.Gln61Lys (c.181C>A)	Unspecified	Acquired during resistance selection process	PubMed=26214590
MutationSimple	FBXW7	p.Arg479Gln (c.1436G>A)	Heterozygous	-	Unknown, Unknown
MutationSimple	CDKN2A	p.His83Tyr (c.247C>T) (p.Ala97Val, c.290C>T)	Unspecified	-	PubMed=11787853

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

DOI PubMed PMC

Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.

van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.

Nat. Commun. 10:3574.1-3574.11(2019).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

Genetic interaction between NRAS and BRAF mutations and PTEN/MMAC1 inactivation in melanoma.

Tsao H., Goel V., Wu H., Yang G., Haluska F.G.

J. Invest. Dermatol. 122:337-341(2004).

DOI PubMed PMC

Relative reciprocity of NRAS and PTEN/MMAC1 alterations in cutaneous melanoma cell lines.

Tsao H., Zhang X., Fowlkes K., Haluska F.G.

Cancer Res. 60:1800-1804(2000).

PubMed

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

Gershwin M.E., Lentz D., Owens R.B.

Exp. Cell Biol. 52:361-370(1984).

DOI PubMed