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A-427Homo sapiens (Human)Cancer cell line

Also known as: A427N, A427

🤖 AI SummaryBased on 13 publications

Quick Overview

Human lung adenocarcinoma cell line with known genetic alterations.

Detailed Summary

The A-427 cell line is a human lung adenocarcinoma cell line derived from a non-small cell lung cancer (NSCLC). It is commonly used in cancer research to study genetic alterations and their implications in tumor development. This cell line has been characterized in multiple studies for its specific mutations and gene expression profiles, making it a valuable model for understanding the molecular mechanisms of lung cancer. Research on A-427 has contributed to the identification of key genetic changes associated with lung carcinogenesis, including mutations in the DCC gene and other critical oncogenes and tumor suppressors. The cell line's genetic profile provides insights into the complex interplay between genetic alterations and cancer progression, offering a platform for testing therapeutic strategies and drug responses.

Research Applications

Genetic Alterations in Lung CancerGene Expression ProfilingMutation AnalysisDrug Response Studies

Key Characteristics

Mutations in DCC GeneExpression of Tumor Suppressor GenesRelevance in Lung Carcinogenesis
Generated on 6/16/2025

Basic Information

Database IDCVCL_1055
SpeciesHomo sapiens (Human)
Tissue SourceLung[UBERON:UBERON_0002048]

Donor Information

Age52
Age CategoryAdult
SexMale

Disease Information

DiseaseLung adenocarcinoma
LineageLung
SubtypeLung Adenocarcinoma
OncoTree CodeLUAD

DepMap Information

Source TypeAcademic lab
Source IDACH-000757_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
MutationNone reportedTP53---PubMed=19787792
MutationSimpleKRASp.Gly12Asp (c.35G>A)Unspecified-PubMed=29786757
MutationSimpleCTNNB1p.Thr41Ala (c.121A>G)Heterozygous-PubMed=36629983

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X,Y
CSF1PO
10,12
D10S1248
13,15
D12S391
18,20
D13S317
11,12
D16S539
11,13
D18S51
12
D19S433
13,14
D1S1656
13,15
D21S11
31.2
D22S1045
16,17
D2S1338
17
D2S441
10
D3S1358
16
D5S818
12
D7S820
8,12
D8S1179
12,13
DYS391
11
FGA
18
Penta D
13
Penta E
15,17
TH01
9
TPOX
8,11
vWA
17
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOIPubMedPMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOIPubMedPMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOIPubMedPMC

Chemistry-first approach for nomination of personalized treatment in lung cancer.

Posner B.A., Minna J.D., Kim H.S., White M.A.

Cell 173:864-878.e29(2018).

DOIPubMedPMC

Differential effector engagement by oncogenic KRAS.";

McCormick F.

Cell Rep. 22:1889-1902(2018).

DOIPubMedPMC

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

DOIPubMedPMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOIPubMedPMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOIPubMedPMC

Comparative proteome analysis across non-small cell lung cancer cell lines.

Daub H.

J. Proteomics 130:1-10(2016).

DOIPubMed

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOIPubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOIPubMed

Human tumor cell strains defective in the repair of alkylation damage.

Mattern M.R.

Carcinogenesis 1:21-32(1980).

DOIPubMed

Prevalence of human papillomavirus 16/18/33 infection and p53 mutation in lung adenocarcinoma.

Iwakawa R., Kohno T., Enari M., Kiyono T., Yokota J.

Cancer Sci. 101:1891-1896(2010).

DOIPubMedPMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOIPubMedPMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOIPubMedPMC

A gene-alteration profile of human lung cancer cell lines.";

Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.

Hum. Mutat. 30:1199-1206(2009).

DOIPubMedPMC

The SRY-HMG box gene, SOX4, is a target of gene amplification at chromosome 6p in lung cancer.

Cigudosa J.C., Lazo P.A., Sanchez-Cespedes M.

Hum. Mol. Genet. 18:1343-1352(2009).

DOIPubMed

Mutation and expression of the DCC gene in human lung cancer.";

Yokota J.

Neoplasia 2:300-305(2000).

DOIPubMedPMC

Analysis of chromosomal alterations in non-small cell lung cancer by multiplex-FISH, comparative genomic hybridization, and multicolor bar coding.

Petersen I.

Lab. Invest. 80:1031-1041(2000).

DOIPubMed

Comprehensive analysis of p53 gene mutation characteristics in lung carcinoma with special reference to histological subtypes.

Fujita T., Kiyama M., Tomizawa Y., Kohno T., Yokota J.

Int. J. Oncol. 15:927-934(1999).

DOIPubMed

Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.

Rousset M., Zweibaum A., Fogh J.

Cancer Res. 41:1165-1170(1981).

PubMed

Oncogenes in solid human tumours.";

Barbacid M.

Nature 300:539-542(1982).

DOIPubMed

Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.

Wright W.C., Daniels W.P., Fogh J.

J. Natl. Cancer Inst. 66:239-247(1981).

DOIPubMed

Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains.

Yarosh D.B., Foote R.S., Mitra S., Day R.S. 3rd

Carcinogenesis 4:199-205(1983).

DOIPubMed

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

Gershwin M.E., Lentz D., Owens R.B.

Exp. Cell Biol. 52:361-370(1984).

DOIPubMed

Cell culture quality control by rapid isoenzymatic characterization.

Halton D.M., Peterson W.D. Jr., Hukku B.

In Vitro 19:16-24(1983).

DOIPubMed

Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.

Lubiniecki A.S., Girardi A.J., Galloway S.M., Bynum G.D.

Nature 288:724-727(1980).

DOIPubMed

In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.

Dosik H., Parks W.P.

J. Natl. Cancer Inst. 51:1417-1423(1973).

DOIPubMed

Four human carcinoma cell lines with novel mutations in position 12 of c-K-ras oncogene.

Valenzuela D.M., Groffen J.

Nucleic Acids Res. 14:843-852(1986).

DOIPubMedPMC

Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.

Brower M., Carney D.N., Oie H.K., Gazdar A.F., Minna J.D.

Cancer Res. 46:798-806(1986).

PubMed

Human tumor lines for cancer research.";

Fogh J.

Cancer Invest. 4:157-184(1986).

DOIPubMed

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Cancer Res. 48:589-601(1988).

PubMed

p53 mutations, ras mutations, and p53-heat shock 70 protein complexes in human lung carcinoma cell lines.

Gerwin B.I., Harris C.C.

Cancer Res. 51:4090-4096(1991).

PubMed

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

Fogh J., Wright W.C., Loveless J.D.

J. Natl. Cancer Inst. 58:209-214(1977).

DOIPubMed

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

Fogh J., Fogh J.M., Orfeo T.

J. Natl. Cancer Inst. 59:221-226(1977).

DOIPubMed

Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.

Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.

Tissue Antigens 11:279-286(1978).

DOIPubMed

Adenovirus: a probe for human cells deficient in DNA repair.";

Day R.S. 3rd

BioScience 31:807-813(1981).

DOI

Web Resources

tcpaportal.org