BHT-101Homo sapiens (Human)Cancer cell line

Also known as: BHT101

🤖 AI SummaryBased on 8 publications

Quick Overview

Human anaplastic thyroid carcinoma cell line for cancer research.

Detailed Summary

BHT-101 is a human anaplastic thyroid carcinoma cell line established from a metastatic lymph node deposit in a female patient. It exhibits heterogeneous cell populations, including epithelial-like and fibroblast-like cells, with a doubling time of 24 hours. The cell line is polyploid with hypertetraploid predominance and shows various chromosomal abnormalities. BHT-101 is sensitive to certain chemotherapeutic agents like adriamycin, methotrexate, and tamoxifen, but not to methimazole. It forms tumors when implanted in immunosuppressed mice and is used for studying thyroid cancer biology and drug sensitivity.

Research Applications

Cancer biology researchDrug sensitivity testingTumor formation studies

Key Characteristics

Polyploid with hypertetraploid predominanceHeterogeneous cell populationSensitivity to adriamycin, methotrexate, and tamoxifenTumor formation in immunosuppressed mice

Generated on 6/16/2025

Basic Information

Database ID	CVCL_1085
Species	Homo sapiens (Human)
Tissue Source	Lymph node[UBERON:UBERON_0000029]

Donor Information

Age	63
Age Category	Adult
Sex	Female

Disease Information

Disease	Anaplastic thyroid carcinoma
Lineage	Thyroid
Subtype	Anaplastic Thyroid Cancer
OncoTree Code	THAP

DepMap Information

Source Type	DSMZ
Source ID	ACH-000191_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Ile251Thr (c.752T>C)	Homozygous	-	Unknown, Unknown, PubMed=30737244
MutationSimple	TERT	c.1-124C>T (c.228C>T) (C228T)	Unspecified	In promoter	from parent cell line Hep-G2
MutationSimple	BRAF	p.Val600Glu (c.1799T>A)	Unspecified	-	PubMed=26214590

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

D13S317

D16S539

9,11

D18S51

12,15

D19S433

13,15

D21S11

29,32.2

D2S1338

20,23

D3S1358

16,17

D5S818

10,11

D7S820

10,11

D8S1179

13,14,15

FGA

18,24

Penta D

Penta E

10,17

TH01

9,9.3

TPOX

vWA

18,19

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Comprehensive genetic characterization of human thyroid cancer cell lines: a validated panel for preclinical studies.

Fagin J.A., Schweppe R.E.

Clin. Cancer Res. 25:3141-3151(2019).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

Frequent somatic TERT promoter mutations in thyroid cancer: higher prevalence in advanced forms of the disease.

Ibrahimpasic T., Ghossein R.A., Fagin J.A.

J. Clin. Endocrinol. Metab. 98:E1562-E1566(2013).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification.

Haugen B.R.

J. Clin. Endocrinol. Metab. 93:4331-4341(2008).

DOI PubMed PMC

Establishment, characterization and drug sensitivity of a new anaplastic thyroid carcinoma cell line (BHT-101).

Palyi I., Peter I., Daubner D., Vincze B., Lorincz I.

Virchows Arch. B. Cell. Pathol. Incl. Mol. Pathol. 63:263-269(1993).

DOI PubMed