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CADO-ES1Homo sapiens (Human)Cancer cell line

Also known as: Center for Adult Diseases Osaka-Ewing Sarcoma 1, ESCADO1, Cado-ES, CADO-ES, CADOES1, CADO ES1, Cado-ES-1, CADO-ES-1

🤖 AI SummaryBased on 13 publications

Quick Overview

CADO-ES1 is a human Ewing sarcoma cell line established from malignant pleural effusion, showing both anchorage-dependent and i...

Detailed Summary

CADO-ES1 is a human Ewing sarcoma cell line derived from the malignant pleural effusion of a 19-year-old woman. This cell line exhibits both anchorage-dependent and anchorage-independent growth, forming multicellular tumor spheroids in culture. It has a nearly diploid DNA content with a DNA index of 0.902 and contains an isodicentric chromosome 8. Despite its undifferentiated appearance, CADO-ES1 shows potential for neural differentiation when treated with cyclic AMP (cAMP). Immunocytochemical analysis reveals strong vimentin expression, with weak positivity for neurofilament (NF) and neuron-specific enolase (NSE). No evidence of Ewing sarcoma-specific translocations, such as t(11;22)(q24;q12), was detected, suggesting a distinct genetic profile. CADO-ES1 is useful for studying Ewing sarcoma biology, differentiation mechanisms, and therapeutic responses.

Research Applications

Cancer biology researchDrug sensitivity testingDifferentiation mechanismsGenomic profiling

Key Characteristics

Anchorage-independent growthMulticellular tumor spheroid formationNearly diploid DNA contentIsodicentric chromosome 8Potential for neural differentiation

Generated on 6/16/2025

Basic Information

Database ID	CVCL_1103
Species	Homo sapiens (Human)
Tissue Source	Pleural effusion[UBERON:UBERON_0000175]

Donor Information

Age	19
Age Category	Adult
Sex	Female
Race	asian
Subtype Features	EWS-ERG

Disease Information

Disease	Ewing sarcoma
Lineage	Bone
Subtype	Ewing Sarcoma
OncoTree Code	ES

DepMap Information

Source Type	DSMZ
Source ID	ACH-000210_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationNone reported	TP53	-	-	-	PubMed=19787792
Gene fusion	ERG	EWSR1-ERG, EWS-ERG	-	In frame	PubMed=9738976
Gene deletion	CDKN2A	-	Homozygous	Possible	PubMed=26870271

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X

CSF1PO

11,12

D13S317

10,13

D16S539

9,11

D18S51

15,20

D19S433

13.2,15.2

D21S11

31,32.2

D2S1338

22,25

D3S1358

16,18

D5S818

11,12

D7S820

11,13

D8S1179

12,15

FGA

21,22

Penta D

13

Penta E

12,19

SE33

19,28.2

TH01

6,9

TPOX

8,11

vWA

14,18

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

Cross-contamination meets misclassification: awakening of CHP-100 from sleeping beauty sleep -- a reviewed model for Ewing's sarcoma.

Nagel S., Steenpass L., Becker J.

Int. J. Cancer 148:2608-2613(2021).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

EZH2 inhibition in Ewing sarcoma upregulates GD2 expression for targeting with gene-modified T cells.

Muller I., Walles H., Hartmann W., Rossig C.

Mol. Ther. 27:933-946(2019).

Defining a characteristic gene expression set responsible for cancer stem cell-like features in a sub-population of Ewing sarcoma cells CADO-ES1.

Hotfilder M., Mallela N., Seggewiss J., Dirksen U., Korsching E.

Int. J. Mol. Sci. 19:3908.1-3908.23(2018).

T cell infiltration into Ewing sarcomas is associated with local expression of immune-inhibitory HLA-G.

Meltzer J., Farwick N., Greune L., Rossig C.

Oncotarget 9:6536-6549(2018).

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients.

Schmidt T., Szuhai K., Hogendoorn P.C.W.

Eur. J. Cancer 51:2624-2633(2015).

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

Breakpoint analysis of transcriptional and genomic profiles uncovers novel gene fusions spanning multiple human cancer types.

West R.B., Pollack J.R.

PLoS Genet. 9:E1003464-E1003464(2013).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

Oncogene mutation profiling of pediatric solid tumors reveals significant subsets of embryonal rhabdomyosarcoma and neuroblastoma with mutated genes in growth signaling pathways.

Borsu L., Barr F.G., Ladanyi M.

Clin. Cancer Res. 18:748-757(2012).

1q gain and CDT2 overexpression underlie an aggressive and highly proliferative form of Ewing sarcoma.

Debiec-Rychter M., Schaefer K.-L., de Alava E.

Oncogene 31:1287-1298(2012).

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.

Buerger H., Aigner T., Gabbert H.E., Poremba C.

Genes Chromosomes Cancer 49:40-51(2010).

Immunocytochemical analysis of cell lines derived from solid tumors.

Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.

J. Histochem. Cytochem. 49:1369-1378(2001).

Establishment and characterization of a new Ewing's sarcoma cell line.

Aoki Y., Misawa S.-i.

Cancer Genet. Cytogenet. 57:19-30(1991).