GI-ME-NHomo sapiens (Human)Cancer cell line

Also known as: Gaslini Institute-ME-Neuroblastoma, Gimen1, Gimen, GIMEN, Gi-MEN, Gi-ME-N

🤖 AI SummaryBased on 15 publications

Quick Overview

Human neuroblastoma cell line with unique genetic features

Detailed Summary

GI-ME-N is a human neuroblastoma cell line established from the metastatic bone marrow of a 2-year-old patient with stage IV neuroblastoma. It exhibits distinct genetic characteristics, including a partial monosomy of the short arm of chromosome 1 (1p) and the absence of N-myc amplification. This cell line has been used in studies investigating the role of genetic alterations in neuroblastoma progression and drug resistance. Research on GI-ME-N has contributed to understanding the molecular mechanisms underlying neuroblastoma, particularly in relation to chromosomal abnormalities and their impact on tumor behavior. The cell line is also utilized in studies examining the effects of various treatments, including cytosine arabinoside, on cell differentiation and growth inhibition.

Research Applications

Genetic studies of neuroblastomaInvestigation of chromosomal abnormalitiesDrug resistance and treatment response studiesCell differentiation and growth inhibition research

Key Characteristics

Partial monosomy of 1pAbsence of N-myc amplificationHigh tumorigenicity in nude miceResponse to cytosine arabinoside treatment

Generated on 6/16/2025

Basic Information

Database ID	CVCL_1232
Species	Homo sapiens (Human)
Tissue Source	Bone marrow[UBERON:UBERON_0002371]

Donor Information

Age	3
Age Category	Pediatric
Sex	Female

Disease Information

Disease	Neuroblastoma
Lineage	Peripheral Nervous System
Subtype	Neuroblastoma
OncoTree Code	NBL

DepMap Information

Source Type	DSMZ
Source ID	ACH-001344_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationUnexplicit	NF1	Microdeletion	Heterozygous	-	from parent cell line GI-ME-N
Gene deletion	NF1	-	Heterozygous	Somatic LOH	PubMed=15207265

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,12

D13S317

8,12

D16S539

9,12

D18S51

12,17

D19S433

13,14

D21S11

D2S1338

19,20

D3S1358

D5S818

D7S820

10,11

D8S1179

10,14

FGA

19,22

Penta D

9,13

Penta E

15,20

TH01

6,7

TPOX

vWA

16,19

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.

Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.

Nature 568:511-516(2019).

DOI PubMed

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

Testing of SNS-032 in a panel of human neuroblastoma cell lines with acquired resistance to a broad range of drugs.

Fichtner I., Ghafourian T., Westermann F., Cinatl J. Jr.

Transl. Oncol. 6:685-696(2013).

DOI PubMed PMC

PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.

Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.

Int. J. Cancer 131:1556-1568(2012).

DOI PubMed PMC

NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.

Messiaen L.M., Versteeg R., Bernards R.

Cell 142:218-229(2010).

DOI PubMed PMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

1H MRS identifies specific metabolite profiles associated with MYCN-amplified and non-amplified tumour subtypes of neuroblastoma cell lines.

Edwards E.C., Strachan M.C., McMullan D.J., Wilkes T.M., Grundy R.G.

NMR Biomed. 20:692-700(2007).

DOI PubMed

Combined M-FISH and CGH analysis allows comprehensive description of genetic alterations in neuroblastoma cell lines.

Salwen H.R., Laureys G., Manoel N., De Paepe A., Speleman F.

Genes Chromosomes Cancer 32:126-135(2001).

DOI PubMed

Analysis of 1;17 translocation breakpoints in neuroblastoma: implications for mapping of neuroblastoma genes.

van der Drift P., Chan A., Versteeg R., Speleman F.

Eur. J. Cancer 33:1974-1978(1997).

DOI PubMed

Comparative genomic hybridization analysis of human neuroblastomas: detection of distal 1p deletions and further molecular genetic characterization of neuroblastoma cell lines.

De Paepe A., Cremer T., Speleman F.

Cancer Genet. Cytogenet. 97:135-142(1997).

DOI PubMed

Deletion mapping in neuroblastoma cell lines suggests two distinct tumor suppressor genes in the 1p35-36 region, only one of which is associated with N-myc amplification.

Speleman F., Versteeg R.

Oncogene 10:291-297(1995).

PubMed

New line of human neuroblastoma derived from bone marrow.";

Repetto G., Cornaglia-Ferraris P.

Pathologica 78:371-384(1986).

PubMed

Cytogenetic and molecular study of two human neuroblastoma cell lines.

Cornaglia-Ferraris P.

Cancer Genet. Cytogenet. 30:225-231(1988).

DOI PubMed

Effect of cytosine arabinoside on the growth and phenotypic expression of GI-ME-N, a new human neuroblastoma cell line.

Cornaglia-Ferraris P.

Prog. Clin. Biol. Res. 271:437-448(1988).

PubMed

N-myc amplification at chromosome band 1p32 in neuroblastoma cells as investigated by in situ hybridization.

Lampert F.

J. Cancer Res. Clin. Oncol. 114:636-640(1988).

DOI PubMed

Morphologic and phenotypic changes of human neuroblastoma cells in culture induced by cytosine arabinoside.

Cornaglia-Ferraris P.

Exp. Cell Res. 181:226-237(1989).

DOI PubMed

Chromosome 1 deletions in human neuroblastomas: generation and fine mapping of microclones from the distal 1p region.

Martinsson T., Weith A., Cziepluch C., Schwab M.

Genes Chromosomes Cancer 1:67-78(1989).

DOI PubMed

A new human highly tumorigenic neuroblastoma cell line with undetectable expression of N-myc.

Donti E., Di Martino D., Tonini G.P.

Pediatr. Res. 27:1-6(1990).

DOI PubMed

Tumor cell lines of the peripheral nervous system.";

Israel M.A., Thiele C.J.

(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.43-78; Academic Press; New York; USA (1994).

DOI

Neuroblastoma.";

Thiele C.J.

(In book chapter) Human cell culture. Vol. 1. Cancer cell lines part 1; Masters J.R.W., Palsson B.O. (eds.); pp.21-53; Kluwer Academic Publishers; New York; USA (1999).

DOI