HuO9Homo sapiens (Human)Cancer cell line

Also known as: Hu09, HUO9, Huo9

🤖 AI SummaryBased on 9 publications

Quick Overview

Human osteosarcoma cell line with metastatic potential

Detailed Summary

The HuO9 cell line is a human osteosarcoma cell line established from a primary tumor in a 13-year-old female patient. It exhibits metastatic potential, particularly to the lungs, and has been used in studies investigating the molecular mechanisms of osteosarcoma progression. This cell line shows characteristics of osteoblastic differentiation, including alkaline phosphatase activity and the production of osteoid. HuO9 has been utilized in research on the role of the Rb gene in tumor pathogenesis and the impact of c-myc gene amplification on cell growth. It is also used to study the effects of various therapeutic agents on metastatic osteosarcoma.

Research Applications

Metastasis studiesOsteoblastic differentiationRb gene analysisc-myc amplification studiesTherapeutic agent testing

Key Characteristics

Metastatic potentialOsteoblastic differentiation markersRb gene alterationsc-myc gene amplification

Generated on 6/16/2025

Basic Information

Database ID	CVCL_1298
Species	Homo sapiens (Human)
Tissue Source	Bone, right distal femur[UBERON:UBERON_0004406]

Donor Information

Age	13
Age Category	Pediatric
Sex	Female

Disease Information

Disease	Osteosarcoma
Lineage	Bone
Subtype	Osteosarcoma
OncoTree Code	OS

DepMap Information

Source Type	JCRB
Source ID	ACH-001526_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationNone reported	TP53	-	-	-	PubMed=19787792

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

10,11

D13S317

D16S539

D18S51

D21S11

D3S1358

D5S818

D7S820

D8S1179

FGA

Penta D

11,13

Penta E

17,21

TH01

TPOX

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Positively selected enhancer elements endow osteosarcoma cells with metastatic competence.

Versteeg H.H., Stamatoyannopoulos J.A., Khanna C., Scacheri P.C.

Nat. Med. 24:176-185(2018).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.

Harris E., Monks A., Morris J.

Mol. Cancer Ther. 14:2452-2462(2015).

DOI PubMed PMC

Characterization of the metastatic phenotype of a panel of established osteosarcoma cells.

Luu H.H., Meltzer P.S., Khanna C.

Oncotarget 6:29469-29481(2015).

DOI PubMed PMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

Identification of human autologous cytotoxic T-lymphocyte-defined osteosarcoma gene that encodes a transcriptional regulator, papillomavirus binding factor.

Nagoya S., Wada T., Yamashita T., Sato N.

Cancer Res. 64:5442-5448(2004).

DOI PubMed

Biological properties and gene expression associated with metastatic potential of human osteosarcoma.

Tsuda H., Aoyagi K., Sasaki H., Ohwada S., Yokota J.

Clin. Exp. Metastasis 20:665-674(2003).

DOI PubMed

Establishment of human osteosarcoma cell lines with high metastatic potential to lungs and their utilities for therapeutic studies on metastatic osteosarcoma.

Moriya H., Yokota J.

Clin. Exp. Metastasis 19:477-485(2002).

DOI PubMed

Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.

Fukaya K.-i., Ishioka C., Namba M.

Acta Med. Okayama 51:261-265(1997).

DOI PubMed

Two distinct cell lines derived from a human osteosarcoma.";

Inoue H.

J. Cancer Res. Clin. Oncol. 115:531-536(1989).

DOI PubMed

A newly established human osteosarcoma cell line with osteoblastic properties.

Kawai A.

Clin. Orthop. Relat. Res. 259:256-267(1990).

DOI PubMed

The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.

Ohashi R., Namba M.

Tissue Cult. Res. Commun. 16:173-178(1997).

DOI