NCC-ITHomo sapiens (Human)Cancer cell line

Also known as: NCCIT, NCC15

🤖 AI SummaryBased on 4 publications

Quick Overview

Human germ cell tumor cell line with cisplatin resistance mutations.

Detailed Summary

The NCC-IT cell line is a human germ cell tumor cell line derived from testicular tissue. It is known for its resistance to cisplatin, a common chemotherapy drug. Research has identified mutations in several genes, including FGFR3, AKT1, PIK3CA, and KRAS, which may contribute to this resistance. These mutations are associated with the PI3K-AKT signaling pathway, suggesting potential targets for therapeutic intervention. The cell line is used in studies investigating the molecular mechanisms of cisplatin resistance and the development of targeted therapies for testicular germ cell tumors.

Research Applications

Cisplatin resistance mechanismsMutational analysis in cancerTargeted therapy development

Key Characteristics

Cisplatin-resistantMutations in PI3K-AKT pathway genesUsed in studying germ cell tumor biology

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1451
Species	Homo sapiens (Human)

Donor Information

Age	24
Age Category	Adult
Sex	Male

Disease Information

Disease	Non-central nervous system-localized embryonal carcinoma
Lineage	Testis
Subtype	Embryonal Carcinoma
OncoTree Code	EMBCA

DepMap Information

Source Type	ATCC
Source ID	ACH-001578_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Val272Cysfs*73 (c.814delG)	Homozygous	-	from parent cell line NCC-IT
MutationSimple	PTEN	p.Arg173Pro (c.518G>C)	Heterozygous	-	from parent cell line NCC-IT
MutationSimple	MAP2K4	p.Lys309Asn (c.927G>C)	Unspecified	-	from parent cell line NCC-IT

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

10,12

D13S317

D16S539

9,12

D18S51

13,14

D21S11

29,32.2

D3S1358

D5S818

10,13

D7S820

D8S1179

10,15

FGA

22,26

Penta D

10,12

Penta E

5,14

TH01

7,9

TPOX

vWA

14,18

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

Proteomic profiling of cisplatin-resistant and cisplatin-sensitive germ cell tumour cell lines using quantitative mass spectrometry.

Strobel P., Kueffer S., Nettersheim D., Bremmer F.

World J. Urol. 40:373-383(2022).

DOI PubMed PMC

Molecular basis of cisplatin resistance in testicular germ cell tumors.

Buchler T., Trka J., Boublikova L.

Cancers (Basel) 11:1316.1-1316.18(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

Presence of somatic mutations within PIK3CA, AKT, RAS, and FGFR3 but not BRAF in cisplatin-resistant germ cell tumors.

Reuter V.E., Bosl G.J., Chaganti R.S.K., Solit D.B.

Clin. Cancer Res. 20:3712-3720(2014).

DOI PubMed

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

Human mitogen-activated protein kinase kinase 4 as a candidate tumor suppressor.

Skolnick M.H., Tavtigian S.V.

Cancer Res. 57:4177-4182(1997).

PubMed

Four new human germ cell tumor cell lines.";

Kanazawa H., Kakizoe T.

Lab. Invest. 59:328-336(1988).

PubMed

Web Resources

de.wikipedia.org