NCI-H146Homo sapiens (Human)Cancer cell line

Also known as: NCIH146, H-146, H146

🤖 AI SummaryBased on 15 publications

Quick Overview

Human small cell lung cancer cell line with known genetic alterations

Detailed Summary

NCI-H146 is a human small cell lung cancer (SCLC) cell line derived from a metastatic lesion. It is widely used in research to study the molecular mechanisms of SCLC, including genetic alterations and therapeutic responses. The cell line exhibits specific mutations and expression profiles that make it a valuable model for investigating SCLC biology and drug development. Its characteristics include a high frequency of RB1 inactivation and other genomic alterations associated with SCLC progression.

Research Applications

Molecular mechanisms of SCLCGenetic alterations in cancerTherapeutic response studies

Key Characteristics

RB1 inactivationHigh genomic instabilityMetastatic origin
Generated on 6/17/2025

Basic Information

Database IDCVCL_1473
SpeciesHomo sapiens (Human)
Tissue SourceBone marrow[UBERON:UBERON_0002371]

Donor Information

Age59
Age CategoryAdult
SexMale
Racecaucasian

Disease Information

DiseaseSmall cell lung cancer
LineageLung
SubtypeSmall Cell Lung Cancer
OncoTree CodeSCLC

DepMap Information

Source TypeATCC
Source IDACH-000506_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
MutationSimpleTP53p.Pro318Leufs*21 (c.953_971del19)Heterozygous-Unknown, Unknown
MutationSimpleRB1p.Gln850Ter (c.2548C>T)Homozygous-Unknown, Unknown
MutationSimpleKMT2Dp.Glu4588Ter (c.13762G>T)Heterozygous-Unknown, Unknown
MutationSimpleKMT2Dp.Glu2911Ter (c.8731G>T)Heterozygous-Unknown, Unknown

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X
CSF1PO
11
D13S317
11,12
D16S539
11
D18S51
15,17
D21S11
30
D3S1358
11
D5S818
12
D7S820
9,10
D8S1179
12,14
FGA
23
Penta D
13
Penta E
12
TH01
6,9.3
TPOX
8,11
vWA
14,16
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.

Aldige C.R., Wistuba I.I., Minna J.D.

J. Cell. Biochem. 121:3986-3999(2020).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.

Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.

OncoImmunology 3:e954893.1-e954893.12(2014).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

Staurosporine and extracellular matrix proteins mediate the conversion of small cell lung carcinoma cells into a neuron-like phenotype.

Winter J., Probstmeier R.

PLoS ONE 9:E86910-E86910(2014).

Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.

Heymach J.V.

Cancer Discov. 2:798-811(2012).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

MicroRNA expression distinguishes SCLC from NSCLC lung tumor cells and suggests a possible pathological relationship between SCLCs and NSCLCs.

Gazdar A.F., Pertsemlidis A.

J. Exp. Clin. Cancer Res. 29:75.1-75.12(2010).

Array comparative genomic hybridization-based characterization of genetic alterations in pulmonary neuroendocrine tumors.

Lucchi M., Smith W.I. Jr., Meltzer P.S., Wang Y.-S., Giaccone G.

Proc. Natl. Acad. Sci. U.S.A. 107:13040-13045(2010).

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.

Sauter G., Lesniewski R., Semizarov D.

Mol. Cancer Res. 5:331-339(2007).

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Oncogene 19:4632-4639(2000).

MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.

Ihde D.C., Gazdar A.F.

J. Cell. Biochem. Suppl. 24:210-217(1996).

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

Insulin-like growth factor expression in human cancer cell lines.";

Grimley C., Battey J., Mulshine J.L., Cuttitta F.

J. Biol. Chem. 271:11477-11483(1996).

Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies.

Gazdar A.F., Minna J.D.

Cancer Res. 44:2052-2061(1984).

High levels of intracellular bombesin characterize human small-cell lung carcinoma.

Moody T.W., Pert C.B., Gazdar A.F., Carney D.N., Minna J.D.

Science 214:1246-1248(1981).

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.

Cuttitta F., Rosen S.T., Gazdar A.F., Minna J.D.

Proc. Natl. Acad. Sci. U.S.A. 78:4591-4595(1981).

Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.

Mitchell J.B.

J. Natl. Cancer Inst. 73:801-807(1984).

Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties.

Bunn P.A. Jr., Guccion J.G., Minna J.D.

Cancer Res. 40:3502-3507(1980).

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Cancer Res. 48:589-601(1988).

Cytogenetics of human small cell lung cancer.";

Whang-Peng J., Lee E.C.

Recent Results Cancer Res. 97:37-46(1985).

Establishment and identification of small cell lung cancer cell lines having classic and variant features.

Moody T.W., Zweig M.H., Minna J.D.

Cancer Res. 45:2913-2923(1985).

Metabolic activation of 4-ipomeanol in human lung, primary pulmonary carcinomas, and established human pulmonary carcinoma cell lines.

Adelberg S., Czerwinski M.J., McMahon N.A., Eggleston J.C., Boyd M.R.

J. Natl. Cancer Inst. 82:1420-1426(1990).

Neuromedin B is present in lung cancer cell lines.";

Giaccone G., Battey J., Gazdar A.F., Oie H.K., Draoui M., Moody T.W.

Cancer Res. 52:2732s-2736s(1992).

Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines.

Burbach J.P.H.

Pathobiology 60:136-142(1992).