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NCI-H196Homo sapiens (Human)Cancer cell line

Also known as: NCIH196, H-196, H196

🤖 AI SummaryBased on 11 publications

Quick Overview

Human small cell lung cancer cell line with Bcl-2 amplification and sensitivity to ABT-737.

Detailed Summary

The NCI-H196 cell line is a human small cell lung cancer (SCLC) cell line derived from a patient with extensive-stage disease. It is characterized by a gain on chromosome 18q, which includes the Bcl-2 gene, and shows sensitivity to the Bcl-2 inhibitor ABT-737. This cell line has been used in studies to identify genomic correlates of drug sensitivity and to explore the role of Bcl-2 amplification in SCLC. The presence of Bcl-2 amplification in NCI-H196 suggests its potential as a model for studying Bcl-2-targeted therapies in SCLC.

Research Applications

Genomic analysis of drug sensitivityBcl-2 amplification studiesTargeted therapy development

Key Characteristics

Bcl-2 gene amplification on 18qSensitivity to ABT-737Extensive-stage disease origin

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1509
Species	Homo sapiens (Human)
Tissue Source	Pleural effusion[UBERON:UBERON_0000175]

Donor Information

Age	68
Age Category	Adult
Sex	Male
Race	caucasian

Disease Information

Disease	Lung sarcomatoid carcinoma
Lineage	Lung
Subtype	SMARCA4-deficient undifferentiated tumor

DepMap Information

Source Type	ATCC
Source ID	ACH-000752_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg175His (c.524G>A)	Unspecified	Somatic mutation acquired during proliferation	from parent cell line YCC-3
MutationSimple	RB1	p.Gln383Ter (c.1147C>T)	Homozygous	-	Unknown

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

10

D12S391

19

D13S317

9

D16S539

11

D18S51

17,19

D19S433

14

D2S1338

17,20

D3S1358

15

D5S818

12

D6S1043

13

D7S820

10,11

D8S1179

13,15

FGA

22,23

Penta D

10

Penta E

8,12

TH01

6

TPOX

11

vWA

19

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Molecular and pathologic characterization of YAP1-expressing small cell lung cancer cell lines leads to reclassification as SMARCA4-deficient malignancies.

Burr M.L., Sutherland K.D.

Clin. Cancer Res. 30:1846-1858(2024).

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.

Aldige C.R., Wistuba I.I., Minna J.D.

J. Cell. Biochem. 121:3986-3999(2020).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.

Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.

OncoImmunology 3:e954893.1-e954893.12(2014).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.

Sauter G., Lesniewski R., Semizarov D.

Mol. Cancer Res. 5:331-339(2007).

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Oncogene 19:4632-4639(2000).

MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.

Ihde D.C., Gazdar A.F.

J. Cell. Biochem. Suppl. 24:210-217(1996).

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

Cytogenetics of human small cell lung cancer.";

Whang-Peng J., Lee E.C.

Recent Results Cancer Res. 97:37-46(1985).

Establishment and identification of small cell lung cancer cell lines having classic and variant features.

Moody T.W., Zweig M.H., Minna J.D.

Cancer Res. 45:2913-2923(1985).