NCI-H2444Homo sapiens (Human)Cancer cell line

Also known as: NCIH244, H-2444, H2444

🤖 AI SummaryBased on 8 publications

Quick Overview

Human lung cancer cell line with known mutations and drug sensitivity profiles.

Detailed Summary

NCI-H2444 is a human lung cancer cell line derived from non-small cell lung cancer (NSCLC). It is widely used in cancer research for studying molecular mechanisms, drug sensitivity, and therapeutic targets. The cell line has been characterized for its genetic mutations, including alterations in the RAS and EGFR pathways, which are critical in cancer progression. Research on NCI-H2444 has contributed to understanding the role of these mutations in tumor development and response to targeted therapies. The cell line is also utilized in high-throughput screening for novel anticancer drugs and in investigating the efficacy of combination therapies. Its availability in large panels like the Cancer Cell Line Encyclopedia (CCLE) and the Cancer Therapeutic Response Portal (CTRP) makes it a valuable resource for preclinical studies.

Research Applications

Drug sensitivity profilingMolecular mechanism studiesTargeted therapy developmentHigh-throughput screeningCombination therapy research

Key Characteristics

RAS pathway mutationsEGFR pathway alterationsDrug response variabilityGenomic instabilityExpression of oncogenic drivers

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1552
Species	Homo sapiens (Human)
Tissue Source	Lung[UBERON:UBERON_0002048]

Donor Information

Age Category	Unknown
Sex	Male

Disease Information

Disease	Lung non-small cell carcinoma
Lineage	Lung
Subtype	Non-Small Cell Lung Cancer
OncoTree Code	NSCLC

DepMap Information

Source Type	ATCC
Source ID	ACH-000186_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Tyr236Cys (c.707A>G)	Heterozygous	-	Unknown, Unknown
MutationSimple	KRAS	p.Gly12Val (c.35G>T)	Heterozygous	Acquired	Unknown, Unknown

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

D13S317

11,12

D16S539

D18S51

D19S433

D21S11

28,29

D2S1338

D3S1358

D5S818

D7S820

D8S1179

11,12,13

FGA

Penta D

9,12

Penta E

TH01

9.3

TPOX

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.

Aldige C.R., Wistuba I.I., Minna J.D.

J. Cell. Biochem. 121:3986-3999(2020).

DOI PubMed PMC

A dataset describing a suite of novel antibody reagents for the RAS signaling network.

Rodriguez H., Whiteaker J.R., Paulovich A.G.

Sci. Data 6:160-160(2019).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Chemistry-first approach for nomination of personalized treatment in lung cancer.

Posner B.A., Minna J.D., Kim H.S., White M.A.

Cell 173:864-878.e29(2018).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

Comparative proteome analysis across non-small cell lung cancer cell lines.

Daub H.

J. Proteomics 130:1-10(2016).

DOI PubMed

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.

Heymach J.V.

Cancer Discov. 2:798-811(2012).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.

Zhou X.-M., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.

Cell 131:1190-1203(2007).

DOI PubMed

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Oncogene 19:4632-4639(2000).

DOI PubMed

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

DOI PubMed