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NCI-H446Homo sapiens (Human)Cancer cell line

Also known as: NCIH446, NCI-446, H-446, H446, NCI-H4466 (Prior to 2020.)

🤖 AI SummaryBased on 13 publications

Quick Overview

Human small cell lung cancer cell line with known genetic alterations

Detailed Summary

The NCI-H446 cell line is a human small cell lung cancer (SCLC) cell line derived from a patient with metastatic disease. It is widely used in cancer research to study the molecular mechanisms of SCLC, including drug resistance and tumor progression. This cell line has been characterized in multiple studies for its genetic and molecular profiles, including mutations in key oncogenes and tumor suppressor genes. Research on NCI-H446 has contributed to understanding the role of specific genetic alterations in SCLC development and response to therapy. The cell line is also utilized in studies investigating the efficacy of targeted therapies and immunotherapies.

Research Applications

Drug resistance mechanismsTumor progressionGenetic alterations in SCLCTargeted therapy developmentImmunotherapy research

Key Characteristics

Metastatic originNeuroendocrine featuresGenetic mutations in key oncogenesUsed in drug sensitivity studies
Generated on 6/17/2025

Basic Information

Database IDCVCL_1562
SpeciesHomo sapiens (Human)
Tissue SourcePleural effusion[UBERON:UBERON_0000175]

Donor Information

Age61
Age CategoryAdult
SexMale
Racecaucasian

Disease Information

DiseaseSmall cell lung cancer
LineageLung
SubtypeSmall Cell Lung Cancer
OncoTree CodeSCLC

DepMap Information

Source TypeATCC
Source IDACH-000800_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
MutationSimpleTP53p.Gly154Val (c.461G>T)Homozygous-Unknown
Gene fusionLY6HPVT1-LY6H--PubMed=23716474

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X,Y
CSF1PO
13,14
D13S317
8
D16S539
12
D18S51
12,13
D19S433
13,14
D21S11
28
D2S1338
18,20
D3S1358
17
D5S818
11
D7S820
10,11
D8S1179
13,15
FGA
22
Penta D
12,13
Penta E
9,10
TH01
8,9.3
TPOX
9,11
vWA
18,19
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOIPubMedPMC

Comparative proteogenomics profiling of non-small and small lung carcinoma cell lines using mass spectrometry.

Wu J.-Y., Hao Z.-F., Ma C., Li P.-F., Dang L.-Y., Sun S.-S.

PeerJ 8:e8779.1-e8779.19(2020).

DOIPubMedPMC

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

DOIPubMedPMC

From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.

Aldige C.R., Wistuba I.I., Minna J.D.

J. Cell. Biochem. 121:3986-3999(2020).

DOIPubMedPMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOIPubMedPMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOIPubMedPMC

Genomic analysis of drug resistant small cell lung cancer cell lines by combining mRNA and miRNA expression profiling.

Chen Y.-T., Yang X., Xu Y.-C., Cao J.-R., Chen L.-B.

Oncol. Lett. 13:4077-4084(2017).

DOIPubMedPMC

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

DOIPubMedPMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOIPubMedPMC

Analysis of novel microRNA targets in drug-sensitive and -insensitive small cell lung cancer cell lines.

Ma L.-J., Li P.-P., Wang R.-X., Nan Y.-D., Liu X.-Y., Jin F.-G.

Oncol. Rep. 35:1611-1621(2016).

DOIPubMed

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOIPubMedPMC

A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.

Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.

OncoImmunology 3:e954893.1-e954893.12(2014).

DOIPubMedPMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOIPubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOIPubMed

Genome-wide identification of genes with amplification and/or fusion in small cell lung cancer.

Yokota J.

Genes Chromosomes Cancer 52:802-816(2013).

DOIPubMedPMC

Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.

Heymach J.V.

Cancer Discov. 2:798-811(2012).

DOIPubMedPMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOIPubMedPMC

Mechanism of multidrug resistance of human small cell lung cancer cell line H446/VP.

Lu Y.-H.

Chin. Med. J. 123:3299-3303(2010).

DOIPubMed

Prevalence of human papillomavirus 16/18/33 infection and p53 mutation in lung adenocarcinoma.

Iwakawa R., Kohno T., Enari M., Kiyono T., Yokota J.

Cancer Sci. 101:1891-1896(2010).

DOIPubMedPMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOIPubMedPMC

Biologic characteristics of the side population of human small cell lung cancer cell line H446.

Wang B., Yang H., Huang Y.-Z., Yan R.-H., Liu F.-J., Zhang J.-N.

Chin. J. Cancer 29:254-260(2010).

DOIPubMed

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOIPubMedPMC

A gene-alteration profile of human lung cancer cell lines.";

Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.

Hum. Mutat. 30:1199-1206(2009).

DOIPubMedPMC

Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.

Sauter G., Lesniewski R., Semizarov D.

Mol. Cancer Res. 5:331-339(2007).

DOIPubMed

Involvement of bcl-2 in multidrug resistance in human small cell lung cancer cell subline H446/DDP.

Huang G.-J.

Zhonghua Jie He He Hu Xi Za Zhi 29:156-160(2006).

DOIPubMed

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Oncogene 19:4632-4639(2000).

DOIPubMed

MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.

Ihde D.C., Gazdar A.F.

J. Cell. Biochem. Suppl. 24:210-217(1996).

DOIPubMed

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

DOIPubMed

Insulin-like growth factor expression in human cancer cell lines.";

Grimley C., Battey J., Mulshine J.L., Cuttitta F.

J. Biol. Chem. 271:11477-11483(1996).

DOIPubMed

Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies.

Gazdar A.F., Minna J.D.

Cancer Res. 44:2052-2061(1984).

PubMed

Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.

Mitchell J.B.

J. Natl. Cancer Inst. 73:801-807(1984).

DOIPubMed

Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties.

Gazdar A.F., Carney D.N., Nau M.M., Minna J.D.

Cancer Res. 45:2924-2930(1985).

PubMed

Establishment and identification of small cell lung cancer cell lines having classic and variant features.

Moody T.W., Zweig M.H., Minna J.D.

Cancer Res. 45:2913-2923(1985).

PubMed

Neuromedin B is present in lung cancer cell lines.";

Giaccone G., Battey J., Gazdar A.F., Oie H.K., Draoui M., Moody T.W.

Cancer Res. 52:2732s-2736s(1992).

PubMed

Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines.

Burbach J.P.H.

Pathobiology 60:136-142(1992).

DOIPubMed

Web Resources

tcpaportal.org