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NCI-H647Homo sapiens (Human)Cancer cell line

Also known as: NCIH647, H647ell, H-647, H647

🤖 AI SummaryBased on 6 publications

Quick Overview

Human lung cancer cell line with known RB status and potential for drug sensitivity studies.

Detailed Summary

The NCI-H647 cell line is a human lung cancer cell line derived from a patient with adenocarcinoma or squamous cell carcinoma. It is characterized by the presence of wild-type RB (retinoblastoma) protein, indicating that the RB pathway is intact. This cell line has been used in studies examining the role of RB-related genes and their interactions with viral oncoproteins such as SV40 large T antigen. The cell line is also part of larger panels like the Cancer Cell Line Encyclopedia (CCLE) and has been utilized in research on drug sensitivity and resistance mechanisms. Its genetic profile and response to various treatments make it a valuable resource for understanding lung cancer biology and developing targeted therapies.

Research Applications

RB pathway analysisDrug sensitivity studiesViral oncoprotein interactionsGenomic profiling

Key Characteristics

Wild-type RB proteinPart of CCLE panelUsed in drug resistance studies

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1574
Species	Homo sapiens (Human)
Tissue Source	Pleural effusion[UBERON:UBERON_0000175]

Donor Information

Age	56
Age Category	Adult
Sex	Male
Race	caucasian

Disease Information

Disease	Lung adenosquamous carcinoma
Lineage	Lung
Subtype	Lung Adenosquamous Carcinoma
OncoTree Code	LUAS

DepMap Information

Source Type	ATCC
Source ID	ACH-000378_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	c.782+1G>T	Homozygous	Splice donor mutation	Unknown, Unknown, PubMed=1565469, PubMed=1311061
MutationSimple	KRAS	p.Gly13Asp (c.38G>A)	Heterozygous	Somatic	from parent cell line MDA-MB-231

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X

CSF1PO

10

D13S317

9,11

D16S539

9

D18S51

12,15

D19S433

14

D21S11

28,32.2

D2S1338

17,25

D3S1358

17

D5S818

12

D7S820

10

D8S1179

11,13

FGA

22,24

Penta D

12,13

Penta E

7

TH01

6,9.3

TPOX

11

vWA

17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.

Aldige C.R., Wistuba I.I., Minna J.D.

J. Cell. Biochem. 121:3986-3999(2020).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

Chemistry-first approach for nomination of personalized treatment in lung cancer.

Posner B.A., Minna J.D., Kim H.S., White M.A.

Cell 173:864-878.e29(2018).

Differential effector engagement by oncogenic KRAS.";

McCormick F.

Cell Rep. 22:1889-1902(2018).

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Oncogene 19:4632-4639(2000).

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Cancer Res. 48:589-601(1988).

Expression of mutant p53 proteins in lung cancer correlates with the class of p53 gene mutation.

Linnoila R.I.

Oncogene 7:743-749(1992).

p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features.

Gazdar A.F.

Oncogene 7:171-180(1992).