NCI-H716Homo sapiens (Human)Cancer cell line

Also known as: NCIH716, H-716, H716, NCI H716

🤖 AI SummaryBased on 13 publications

Quick Overview

Human colorectal cancer cell line with known genetic alterations

Detailed Summary

The NCI-H716 cell line is a human colorectal cancer cell line derived from a primary tumor. It is widely used in cancer research to study the molecular mechanisms of colorectal cancer and to evaluate the efficacy of potential therapeutic agents. This cell line has been characterized in multiple studies, including its genetic profile, drug sensitivity, and response to various treatments. Research on NCI-H716 has contributed to understanding the role of specific mutations and pathways in cancer progression and treatment resistance.

Research Applications

Cancer researchDrug sensitivity testingMolecular mechanism studies

Key Characteristics

Genetic alterationsDrug response profilingMolecular pathway analysis
Generated on 6/17/2025

Basic Information

Database IDCVCL_1581
SpeciesHomo sapiens (Human)
Tissue SourceAscites[UBERON:UBERON_0007795]

Donor Information

Age33
Age CategoryAdult
SexMale
Racecaucasian

Disease Information

DiseaseCecum adenocarcinoma
LineageBowel
SubtypeColon Adenocarcinoma
OncoTree CodeCOAD

DepMap Information

Source TypeATCC
Source IDACH-000491_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
MutationSimpleTP53p.Glu224Asp (c.672G>T)Homozygous-Unknown, Unknown, PubMed=16418264
MutationSimpleKRASp.Arg97Ile (c.290G>T)Heterozygous-Unknown, Unknown
Gene fusionNPLOC4NPLOC4-PDE6G--PubMed=25485619

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X
CSF1PO
11
D13S317
8,11
D16S539
11,12
D18S51
15,16
D19S433
15,18.2
D21S11
32.2
D2S1338
14,16
D3S1358
16,18
D5S818
11
D7S820
10,11
D8S1179
12.2,14,15
FGA
20,22
Penta D
9
Penta E
7,12
TH01
6,9.3
TPOX
8,11
vWA
16
Gene Expression Profile
Gene expression levels and statistical distribution
Loading cohorts...
Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

NCI-H716 cells.";

Gagnon J., Brubaker P.L.

(In book chapter) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx K., Cotter P., Lopez-Exposito I., Kleiveland C., Lea T., Mackie A., Requena T., Swiatecka D., Wichers H. (eds.); pp.221-228; Springer; Cham; Switzerland (2015).

Differential effector engagement by oncogenic KRAS.";

McCormick F.

Cell Rep. 22:1889-1902(2018).

Pharmacoproteomic characterisation of human colon and rectal cancer.

Weichert W., Knapp S., Feller S.M., Kuster B.

Mol. Syst. Biol. 13:951-951(2017).

Genomic determinants of protein abundance variation in colorectal cancer cells.

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Choudhary J.S.

Cell Rep. 20:2201-2214(2017).

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.

Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.

Nat. Commun. 6:7002.1-7002.10(2015).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.

Mariadason J.M., Sieber O.M.

Cancer Res. 74:3238-3247(2014).

Subtypes of primary colorectal tumors correlate with response to targeted treatment in colorectal cell lines.

Orphanides G., French T., Wessels L.F.A.

BMC Med. Genomics 5:66.1-66.15(2012).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.

Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.

Br. J. Cancer 103:340-346(2010).

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines.

Camps J., McNeil N.E., Difilippantonio M.J., Ried T.

Genes Chromosomes Cancer 49:204-223(2010).

Cell growth, global phosphotyrosine elevation, and c-Met phosphorylation through Src family kinases in colorectal cancer cells.

Emaduddin M., Bicknell D.C., Bodmer W.F., Feller S.M.

Proc. Natl. Acad. Sci. U.S.A. 105:2358-2362(2008).

Analysis of p53 mutations and their expression in 56 colorectal cancer cell lines.

Liu Y., Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006).

Biology of colorectal and gastric cancer cell lines.";

Park J.-G., Gazdar A.F.

J. Cell. Biochem. Suppl. 24:131-141(1996).

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

NCI series of cell lines: an historical perspective.";

Gazdar A.F., Minna J.D.

J. Cell. Biochem. 63 Suppl. 24:1-11(1996).

Characteristics of cell lines established from human colorectal carcinoma.

Johnson B.E., Gazdar A.F.

Cancer Res. 47:6710-6718(1987).

NCI-H716 cells as a model for endocrine differentiation in colorectal cancer.

Rousch M.J.M., Moerkerk P.T.M., de Goeij A.F.P.M., Bosman F.T.

Virchows Arch. B. Cell. Pathol. Incl. Mol. Pathol. 62:311-320(1992).

Web Resources