NCI-H748Homo sapiens (Human)Cancer cell line

Also known as: NCIH748, H-748, H748

🤖 AI SummaryBased on 7 publications

Quick Overview

Human small cell lung cancer cell line with Bcl-2 amplification and sensitivity to Bcl-2 inhibitors.

Detailed Summary

NCI-H748 is a human small cell lung cancer (SCLC) cell line derived from a patient with extensive-stage disease. It is characterized by a gain on chromosome 18q, which includes the Bcl-2 and NOXA genes, and exhibits sensitivity to Bcl-2 family inhibitors like ABT-737. This cell line has been used in studies to identify genomic markers of drug sensitivity and to explore the role of Bcl-2 amplification in cancer progression. The presence of this amplification correlates with increased sensitivity to Bcl-2-targeting therapies, making NCI-H748 a valuable model for studying the molecular mechanisms of drug response in SCLC.

Research Applications

Genomic biomarker discovery for drug sensitivityBcl-2 family inhibitor sensitivity testingAmplification analysis of 18q regionMolecular mechanisms of apoptosis regulation

Key Characteristics

Amplification of Bcl-2 and NOXA on 18qSensitivity to ABT-737 and other Bcl-2 inhibitorsExtensive-stage disease originOverexpression of Bcl-2 and NOXA genes

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1588
Species	Homo sapiens (Human)
Tissue Source	Lymph node[UBERON:UBERON_0000029]

Donor Information

Age	62
Age Category	Adult
Sex	Male

Disease Information

Disease	Small cell lung cancer
Lineage	Lung
Subtype	Small Cell Lung Cancer
OncoTree Code	SCLC

DepMap Information

Source Type	ATCC
Source ID	ACH-003071_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Tyr220Cys (c.659A>G)	Unspecified	-	PubMed=21173094
MutationSimple	RB1	p.Ile324fs (c.967_968insA)	Homozygous	-	Unknown
MutationSimple	BRCA2	p.Asp2237Metfs*4 (c.6709del)	Heterozygous	-	Unknown, Unknown

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

D13S317

D16S539

D18S51

D19S433

12,12.2

D21S11

D2S1338

D3S1358

D5S818

D7S820

10,11

D8S1179

FGA

25,27

Penta D

Penta E

TH01

7,9

TPOX

10,12

vWA

17,19

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.

Aldige C.R., Wistuba I.I., Minna J.D.

J. Cell. Biochem. 121:3986-3999(2020).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.

Sauter G., Lesniewski R., Semizarov D.

Mol. Cancer Res. 5:331-339(2007).

DOI PubMed

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Oncogene 19:4632-4639(2000).

DOI PubMed

MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.

Ihde D.C., Gazdar A.F.

J. Cell. Biochem. Suppl. 24:210-217(1996).

DOI PubMed

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

DOI PubMed