NCI-H774Homo sapiens (Human)Cancer cell line

Also known as: H-774, H774

🤖 AI SummaryBased on 7 publications

Quick Overview

Human lung cancer cell line with known p53 mutations and IGF expression.

Detailed Summary

The NCI-H774 cell line is a human lung cancer cell line derived from small cell lung cancer (SCLC). It is characterized by specific genetic alterations, including mutations in the p53 gene and expression of insulin-like growth factor (IGF) proteins. Research on NCI-H774 has focused on understanding the role of these genetic changes in cancer progression and therapeutic resistance. The cell line has been used in studies examining the mutational landscape of lung cancer, particularly in relation to histological subtypes and smoking history. Additionally, NCI-H774 has been involved in investigations into the RB-related gene family and DCC gene alterations, contributing to the understanding of tumor suppressor mechanisms in lung carcinogenesis.

Research Applications

Analysis of p53 gene mutationsIGF expression studiesRB-related gene and DCC gene alterationsInvestigation of tumor suppressor mechanisms

Key Characteristics

p53 gene mutationsIGF-I and IGF-II expressionRB-related gene inactivationDCC gene expression variability

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1589
Species	Homo sapiens (Human)
Tissue Source	Soft tissue[UBERON:UBERON_0034929]

Donor Information

Age	43
Age Category	Adult
Sex	Male

Disease Information

Disease	Small cell lung cancer
Lineage	Lung
Subtype	Small Cell Lung Cancer
OncoTree Code	SCLC

DepMap Information

Source Type	ATCC
Source ID	ACH-003061_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg342Ter (c.1024C>T)	Homozygous	-	PubMed=29970484
Gene deletion	MAP2K4	-	Homozygous	-	PubMed=9331070

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

D13S317

D16S539

D18S51

14,17

D19S433

13,14

D21S11

31.2

D2S1338

23,24

D3S1358

D5S818

D7S820

9,11

D8S1179

12,13

FGA

Penta D

Penta E

7,12

TH01

6,9.3

TPOX

vWA

15,17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.

Aldige C.R., Wistuba I.I., Minna J.D.

J. Cell. Biochem. 121:3986-3999(2020).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Oncogene 19:4632-4639(2000).

DOI PubMed

Mutation and expression of the DCC gene in human lung cancer.";

Yokota J.

Neoplasia 2:300-305(2000).

DOI PubMed PMC

Comprehensive analysis of p53 gene mutation characteristics in lung carcinoma with special reference to histological subtypes.

Fujita T., Kiyama M., Tomizawa Y., Kohno T., Yokota J.

Int. J. Oncol. 15:927-934(1999).

DOI PubMed

Human mitogen-activated protein kinase kinase 4 as a candidate tumor suppressor.

Skolnick M.H., Tavtigian S.V.

Cancer Res. 57:4177-4182(1997).

PubMed

MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.

Ihde D.C., Gazdar A.F.

J. Cell. Biochem. Suppl. 24:210-217(1996).

DOI PubMed

NCI-Navy Medical Oncology Branch cell line data base.";

Carney D.N., Minna J.D., Mulshine J.L.

J. Cell. Biochem. Suppl. 24:32-91(1996).

DOI PubMed

Insulin-like growth factor expression in human cancer cell lines.";

Grimley C., Battey J., Mulshine J.L., Cuttitta F.

J. Biol. Chem. 271:11477-11483(1996).

DOI PubMed

Expression of mutant p53 proteins in lung cancer correlates with the class of p53 gene mutation.

Linnoila R.I.

Oncogene 7:743-749(1992).

PubMed

High frequency of somatically acquired p53 mutations in small-cell lung cancer cell lines and tumors.

Gazdar A.F., Minna J.D.

Oncogene 7:339-346(1992).

PubMed