RPMI-2650Homo sapiens (Human)Cancer cell line

Also known as: Roswell Park Memorial Institute 2650, R.P.M.I. #2650, RPMI2650, RPMI 2650, CCL30 (Based on the ATCC catalog number.)

🤖 AI SummaryBased on 12 publications

Quick Overview

Human nasopharyngeal carcinoma cell line with quasi-diploid karyotype.

Detailed Summary

RPMI-2650 is a human nasopharyngeal carcinoma cell line established from a pleural effusion. It exhibits a quasi-diploid karyotype with 46 chromosomes, though it contains a consistent minor rearrangement involving the D group chromosomes. This cell line has been cultured in vitro for over two years and demonstrates stable chromosome numbers, making it a valuable model for studying tumor biology and genetic stability. RPMI-2650 is used in research related to cancer genetics, tumor formation, and drug response studies due to its stable in vitro characteristics and relevance to human malignancies.

Key Characteristics

Quasi-diploid karyotypeStable chromosome numberExpresses human papillavirus sequencesUsed in tumor formation studies

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1664
Species	Homo sapiens (Human)
Tissue Source	Pleural effusion[UBERON:UBERON_0000175]

Donor Information

Age	52
Age Category	Adult
Sex	Male

Disease Information

Disease	NUT midline carcinoma
Lineage	Head and Neck
Subtype	NUT Midline Carcinoma of the Head and Neck
OncoTree Code	NMCHN

DepMap Information

Source Type	ATCC
Source ID	ACH-001385_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
Gene fusion	BRD4	BRD4-NUTM1, BRD4-NUT	-	BRD4 exon 14 fused to NUTM1 exon 2	PubMed=29348827, PubMed=28203693, PubMed=24736545

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

9,11

D13S317

11,12

D16S539

11,12

D18S51

D19S433

14,16

D21S11

28,33.2

D2S1338

20,25

D3S1358

D5S818

12,13

D7S820

8,11

D8S1179

9,13

FGA

23,25

Penta D

9,10

Penta E

11,19

TH01

6,8

TPOX

vWA

16,18

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Molecular-genetic profiling and high-throughput in vitro drug screening in NUT midline carcinoma-an aggressive and fatal disease.

Stirnweiss A., Oommen J., Kotecha R.S., Kees U.R., Beesley A.H.

Oncotarget 8:112313-112329(2017).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOI PubMed

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Growth of human tumor cells in suspension cultures.";

Moore G.E., Mount D., Tara G., Schwartz N.

Cancer Res. 23:1735-1741(1963).

PubMed

Presence of 1,25-dihydroxyvitamin D3 receptors in established human cancer cell lines in culture.

Moseley J.M., Martin T.J.

Cancer Res. 42:1116-1119(1982).

PubMed

Human tumor cell line with a quasi-diploid karyotype (RPMI 2650).";

Moorhead P.S.

Exp. Cell Res. 39:190-196(1965).

DOI PubMed

Human tumor lines for cancer research.";

Fogh J.

Cancer Invest. 4:157-184(1986).

DOI PubMed

Growth control of heterologous tissue culture cells in the congenitally athymic nude mouse.

Stiles C.D., Desmond W. Jr., Chuman L.M., Sato G.H., Saier M.H. Jr.

Cancer Res. 36:1353-1360(1976).

PubMed

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

Fogh J., Wright W.C., Loveless J.D.

J. Natl. Cancer Inst. 58:209-214(1977).

DOI PubMed

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

Fogh J., Fogh J.M., Orfeo T.

J. Natl. Cancer Inst. 59:221-226(1977).

DOI PubMed

Failure of human cells transformed by simian virus 40 to form tumors in athymic nude mice.

Stiles C.D., Desmond W. Jr., Sato G.H., Saier M.H. Jr.

Proc. Natl. Acad. Sci. U.S.A. 72:4971-4975(1975).

DOI PubMed PMC

Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.

Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.

Tissue Antigens 11:279-286(1978).

DOI PubMed

Head and neck tumor cell lines.";

Carey T.E.

(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.79-120; Academic Press; New York; USA (1994).

DOI

The animal cell culture collection.";

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.

In Vitro 5:1-16(1970).

DOI

Chromosome abnormalities in human tumor cells in culture.";

Biedler J.L.

(In book chapter) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York; USA (1975).

DOI