SH-4Homo sapiens (Human)Cancer cell line

Also known as: SH4

🤖 AI SummaryBased on 11 publications

Quick Overview

SH-4 is a melanoma cell line derived from pleural effusion, used for cancer research.

Detailed Summary

SH-4 is a human melanoma cell line established from the pleural effusion of a patient with metastatic melanoma. It was developed after a period of slow growth, leading to the emergence of a continuous, rapidly growing cell line. The cells exhibit spindle-shaped or oligodendritic morphology and show partial pigmentation. Chromosome analysis indicates a near-diploid karyotype with a modal number of 51. SH-4 has been utilized in studies related to melanoma biology, including investigations into pigment synthesis and cellular differentiation. Its establishment provides a valuable model for studying the molecular mechanisms of melanoma progression and therapeutic responses.

Research Applications

Cancer researchMelanoma biologyPigment synthesis studiesCellular differentiationTherapeutic response investigations

Key Characteristics

Spindle-shaped or oligodendritic morphologyPartial pigmentationNear-diploid karyotypeRapid growth after initial slow phase

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1692
Species	Homo sapiens (Human)
Tissue Source	Pleural effusion[UBERON:UBERON_0000175]

Donor Information

Age	60
Age Category	Adult
Sex	Female
Race	caucasian

Disease Information

Disease	Melanoma
Lineage	Skin
Subtype	Melanoma
OncoTree Code	MEL

DepMap Information

Source Type	ATCC
Source ID	ACH-000441_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	BRAF	p.Val600Glu (c.1799T>A)	Unspecified	-	PubMed=26214590

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,12

D13S317

9,12

D16S539

11,12

D18S51

17,19

D19S433

13,14

D21S11

31.2

D2S1338

16,20

D3S1358

15,16

D5S818

11,12

D7S820

D8S1179

13,15

FGA

20,21

Penta D

Penta E

10,18

TH01

8,9.3

TPOX

8,11

vWA

16,17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Signatures of protein expression revealed by secretome analyses of cancer associated fibroblasts and melanoma cell lines.

Zelanis A.

J. Proteomics 174:1-8(2018).

DOI PubMed

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

Mutations of the BRAF gene in human cancer.";

Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.

Nature 417:949-954(2002).

DOI PubMed

Human tumor lines for cancer research.";

Fogh J.

Cancer Invest. 4:157-184(1986).

DOI PubMed

Establishment of a cell line (SH-4) from pleural effusion of a patient with melanoma.

Seman G., Hunter S.J., Lukeman J.M., Dmochowski L.

In Vitro 11:205-211(1975).

DOI PubMed

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

Fogh J., Wright W.C., Loveless J.D.

J. Natl. Cancer Inst. 58:209-214(1977).

DOI PubMed

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

Fogh J., Fogh J.M., Orfeo T.

J. Natl. Cancer Inst. 59:221-226(1977).

DOI PubMed