CAL-29Homo sapiens (Human)Cancer cell line

Also known as: Centre Antoine Lacassagne-29, CAL29, CAL 29

🤖 AI SummaryBased on 8 publications

Quick Overview

CAL-29 is a human bladder cancer cell line established from a high-grade transitional cell carcinoma, known for its tumorigenic...

Detailed Summary

CAL-29 is a human bladder cancer cell line derived from a high-grade transitional cell carcinoma, characterized by its tumorigenic potential in nude mice and its ability to undergo epithelial-mesenchymal transition (EMT) when exposed to growth factors such as EGF and TGF-α. These cells exhibit distinct morphological changes, including scattering and loss of epithelial markers, which are reversible upon removal of the growth factors. CAL-29 is widely used in research to study the mechanisms of cancer invasion and metastasis, particularly focusing on the role of EMT in tumor progression. The cell line is also valuable for investigating the effects of various kinase inhibitors on cell scattering and EMT, providing insights into potential therapeutic strategies for bladder cancer.

Research Applications

Tumorigenicity studiesEpithelial-mesenchymal transition (EMT) researchDrug response profilingKinase inhibitor studies

Key Characteristics

Tumorigenic in nude miceResponsive to EGF and TGF-α for EMTReversible cell scatteringLoss of epithelial markers

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1808
Species	Homo sapiens (Human)
Tissue Source	Urinary bladder[UBERON:UBERON_0001255]

Donor Information

Age	80
Age Category	Adult
Sex	Female

Disease Information

Disease	Bladder carcinoma
Lineage	Bladder/Urinary Tract
Subtype	Bladder Urothelial Carcinoma
OncoTree Code	BLCA

DepMap Information

Source Type	DSMZ
Source ID	ACH-000142_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg282Trp (c.844C>T)	Unspecified	-	PubMed=21173094, PubMed=1373872
MutationSimple	TERT	c.1-124C>T (c.228C>T) (C228T)	Unspecified	In promoter	from parent cell line Hep-G2
MutationSimple	PIK3CA	p.His1047Arg (c.3140A>G)	Unspecified	-	PubMed=25926053, PubMed=20570890

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

10,12

D13S317

8,12

D16S539

D18S51

13,18

D19S433

12,14

D21S11

31,33.2

D2S1338

20,25

D3S1358

18,19

D5S818

11,12

D7S820

8,11

D8S1179

FGA

23,24

Penta D

9,10

Penta E

5,13

TH01

TPOX

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.

van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.

Nat. Commun. 10:3574.1-3574.11(2019).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Systematic review: characteristics and preclinical uses of bladder cancer cell lines.

Zuiverloon T.C.M., de Jong F.C., Costello J.C., Theodorescu D.

Bladder Cancer 4:169-183(2018).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

Identification of mutations in distinct regions of p85 alpha in urothelial cancer.

Knowles M.A.

PLoS ONE 8:E84411-E84411(2013).

DOI PubMed PMC

Comprehensive mutation analysis of the TERT promoter in bladder cancer and detection of mutations in voided urine.

Hurst C.D., Platt F.M., Knowles M.A.

Eur. Urol. 65:367-369(2014).

DOI PubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Establishment of two new human bladder carcinoma cell lines, CAL 29 and CAL 185. Comparative study of cell scattering and epithelial to mesenchyme transition induced by growth factors.

Stora de Novion H., Amiel J., Lagrange J.-L., Rossi B., Gioanni J.

Br. J. Cancer 85:1412-1417(2001).

DOI PubMed PMC

Characterization of a new surface epitope specific for human epithelial cells defined by a monoclonal antibody and application to tumor diagnosis.

Lalanne C.-M.

Cancer Res. 47:4417-4424(1987).

PubMed