PaCaDD-135Homo sapiens (Human)Cancer cell line
Also known as: PACADD135, PACADD-135
🤖 AI SummaryBased on 1 publications
Quick Overview
Human pancreatic cancer cell line for research on PDAC.
Detailed Summary
PaCaDD-135 is a human pancreatic ductal adenocarcinoma (PDAC) cell line established from a lymph node metastasis. It exhibits epithelial morphology with a cobblestone pattern and demonstrates a doubling time of approximately 43.83 hours. The cell line shows cytoplasmic expression of CK8/18, membrane and cytoplasmic expression of E-cadherin, and cytoplasmic expression of ezrin. KRAS mutations are present, consistent with the primary tumor. PaCaDD-135 is used in studies focusing on PDAC biology, including investigations into cell growth, invasion, and molecular pathways such as EMT and apoptosis. It is maintained in Dresden-medium and has been characterized for its immunohistochemical profile and genetic mutations.
Research Applications
Cancer biologyPancreatic cancer researchCell proliferationInvasion and metastasisMolecular pathways in PDAC
Key Characteristics
Epithelial morphologyCobblestone patternKRAS mutationCytoplasmic CK8/18 expressionMembrane and cytoplasmic E-cadherin expressionCytoplasmic ezrin expressionDresden-medium culturingIn vitro growth characteristics
Generated on 6/17/2025
Basic Information
| Database ID | CVCL_1849 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Lymph node[UBERON:UBERON_0000029] |
Donor Information
| Age | 48 |
|---|---|
| Age Category | Adult |
| Sex | Female |
Disease Information
| Disease | Pancreatic adenocarcinoma |
|---|---|
| Lineage | Pancreas |
| Subtype | Pancreatic Adenocarcinoma |
| OncoTree Code | PAAD |
DepMap Information
| Source Type | DSMZ |
|---|---|
| Source ID | ACH-001376_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.Arg249fs (c.746_750_delGGCCC) | Unspecified | - | Unknown |
| MutationSimple | KRAS | p.Gly12Val (c.35G>T) | Heterozygous | Acquired | Unknown, Unknown |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
10
D13S317
11,13
D16S539
11,12
D18S51
17
D19S433
13,15
D21S11
29
D2S1338
18,20
D3S1358
15,16
D5S818
11,12
D7S820
11,12
D8S1179
13
FGA
19
Penta D
2,12
Penta E
5,11
TH01
9,9.3
TPOX
8,11
vWA
14,19
Gene Expression Profile
Gene expression levels and statistical distribution
Loading cohorts...
Full DepMap dataset with combined data across cell lines
Loading gene expression data...
Publications
Five primary human pancreatic adenocarcinoma cell lines established by the outgrowth method.
Krautz C., Hering S., Saeger H.-D., Grutzmann R., Pilarsky C.
J. Surg. Res. 172:29-39(2012).
Distribution of characteristic mutations in native ductal adenocarcinoma of the pancreas and pancreatic cancer cell lines.
Saeger H.-D.
Cell Biol. Res. Ther. 2:1000104.1-1000104.5(2013).