PaCaDD-137Homo sapiens (Human)Cancer cell line
Also known as: PACADD137, PACADD-137
🤖 AI SummaryBased on 1 publications
Quick Overview
Human pancreatic cancer cell line for research
Detailed Summary
PaCaDD-137 is a human pancreatic ductal adenocarcinoma cell line established using the Dresden outgrowth method. It exhibits epithelial-like morphology with a homogenous, colony-like pattern. The cell line shows a doubling time of 24.24 hours and does not adhere to matrigel, indicating limited invasive potential. Immunocytochemical analysis reveals cytoplasmic expression of CK8/18, membrane and cytoplasmic expression of E-cadherin, and nuclear p53 accumulation. KRAS mutations are present, with the cell line showing G12D mutation. PaCaDD-137 is used in studies related to pancreatic cancer biology, including investigations into cell proliferation, invasion, and molecular pathways such as EMT and apoptosis.
Research Applications
Pancreatic cancer researchCell proliferation studiesInvasion and metastasis researchMolecular pathway analysis
Key Characteristics
Epithelial-like morphologyDoubling time: 24.24 hoursNo matrigel adhesionCytoplasmic CK8/18 expressionMembrane and cytoplasmic E-cadherinNuclear p53 accumulationKRAS G12D mutation
Generated on 6/17/2025
Basic Information
| Database ID | CVCL_1850 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Pancreas, head[UBERON:UBERON_0001069] |
Donor Information
| Age | 75 |
|---|---|
| Age Category | Adult |
| Sex | Female |
Disease Information
| Disease | Pancreatic adenocarcinoma |
|---|---|
| Lineage | Pancreas |
| Subtype | Pancreatic Adenocarcinoma |
| OncoTree Code | PAAD |
DepMap Information
| Source Type | DSMZ |
|---|---|
| Source ID | ACH-001377_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | PTEN | p.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA) | Heterozygous | - | Unknown, Unknown, PubMed=25230021 |
| MutationSimple | CDKN2A | p.Arg80Ter (c.237_238CC>TT) (c.237_238delinsTT) (p.Pro94Leu, c.280_281CC>TT) | Unspecified | - | PubMed=23851445 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
11,12
D13S317
11
D16S539
11,12
D18S51
14
D19S433
12,13
D21S11
29.2,32.2
D2S1338
18,19
D3S1358
15,16
D5S818
9,12
D7S820
9,10
D8S1179
10
FGA
25,26
Penta D
9,11
Penta E
12,17
TH01
9,9.3
TPOX
8
vWA
18,19
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Five primary human pancreatic adenocarcinoma cell lines established by the outgrowth method.
Krautz C., Hering S., Saeger H.-D., Grutzmann R., Pilarsky C.
J. Surg. Res. 172:29-39(2012).
Distribution of characteristic mutations in native ductal adenocarcinoma of the pancreas and pancreatic cancer cell lines.
Saeger H.-D.
Cell Biol. Res. Ther. 2:1000104.1-1000104.5(2013).