COLO 201Homo sapiens (Human)Cancer cell line

Also known as: Colorado 201, Colo201, COLO201, COLO-201, Colo-201, Colo 201, Colo210

🤖 AI SummaryBased on 16 publications

Quick Overview

Human colorectal cancer cell line with known mutations and drug response profiles.

Detailed Summary

COLO 201 is a human colorectal cancer cell line derived from a primary tumor. It is widely used in cancer research to study molecular mechanisms and drug responses. The cell line exhibits specific genetic alterations, including mutations in the APC gene, which are common in colorectal cancers. COLO 201 has been utilized in studies investigating the effects of 5-fluorouracil (5-FU) and other chemotherapeutic agents, showing variable sensitivity to these treatments. Research on COLO 201 has contributed to understanding the role of mismatch repair deficiency in drug response and tumor biology. Additionally, it has been part of studies examining the genetic and molecular characteristics of colorectal cancer, including the identification of key mutations and pathways involved in tumorigenesis.

Research Applications

Study of colorectal cancer molecular mechanismsDrug response profilingInvestigation of APC gene mutationsAnalysis of 5-fluorouracil sensitivityResearch on mismatch repair deficiencyGenetic and molecular characterization of colorectal tumors

Key Characteristics

APC gene mutationsVariable sensitivity to 5-FUMismatch repair deficiency associationUsed in drug response studies

Generated on 6/17/2025

Basic Information

Database ID	CVCL_1987
Species	Homo sapiens (Human)
Tissue Source	Ascites[UBERON:UBERON_0007795]

Donor Information

Age	70
Age Category	Adult
Sex	Male
Race	caucasian

Disease Information

Disease	Colon adenocarcinoma
Lineage	Bowel
Subtype	Colon Adenocarcinoma
OncoTree Code	COAD

DepMap Information

Source Type	ATCC
Source ID	ACH-000253_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	CTNNB1	p.Asn287Ser (c.860A>G)	Homozygous	-	from parent cell line COLO 201
MutationSimple	BRAF	p.Val600Glu (c.1799T>A)	Unspecified	-	PubMed=26214590
MutationSimple	APC	p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA)	Heterozygous	-	from parent cell line WiDr

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,12

D13S317

D16S539

12,13

D18S51

D19S433

13,14

D21S11

30.2,33.2

D2S1338

17,18

D3S1358

D5S818

10,13

D7S820

9,10

D8S1179

9,14

FGA

21,23

Penta D

9,11

Penta E

13,15

TH01

8,9

TPOX

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

Highly expressed genes in rapidly proliferating tumor cells as new targets for colorectal cancer treatment.

Sanchez A., Schwartz S. Jr., Bilic J., Mariadason J.M., Arango D.

Clin. Cancer Res. 21:3695-3704(2015).

DOI PubMed

The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.

Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.

Nat. Commun. 6:7002.1-7002.10(2015).

DOI PubMed

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOI PubMed

Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.

Mariadason J.M., Sieber O.M.

Cancer Res. 74:3238-3247(2014).

DOI PubMed

Subtypes of primary colorectal tumors correlate with response to targeted treatment in colorectal cell lines.

Orphanides G., French T., Wessels L.F.A.

BMC Med. Genomics 5:66.1-66.15(2012).

DOI PubMed PMC

5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.

Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.

Br. J. Cancer 103:340-346(2010).

DOI PubMed PMC

Genomic and biological characterization of exon 4 KRAS mutations in human cancer.

Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.

Cancer Res. 70:5901-5911(2010).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Analysis of p53 mutations and their expression in 56 colorectal cancer cell lines.

Liu Y., Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006).

DOI PubMed PMC

Short tandem repeat profiling provides an international reference standard for human cell lines.

Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).

DOI PubMed PMC

Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.

Wolf E., Gabius H.-J.

J. Cancer Res. Clin. Oncol. 127:375-386(2001).

DOI PubMed

Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations.

Neumann T., Jauho A., Auer G., Ried T.

Genes Chromosomes Cancer 27:183-190(2000).

DOI PubMed PMC

Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.

Pignatelli M., Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 96:2316-2321(1999).

DOI PubMed PMC

Beta-catenin mutations in cell lines established from human colorectal cancers.

Ilyas M., Tomlinson I.P.M., Rowan A.J., Pignatelli M., Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 94:10330-10334(1997).

DOI PubMed PMC

Screening the p53 status of human cell lines using a yeast functional assay.

Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.

Mol. Carcinog. 19:243-253(1997).

DOI PubMed

HLA-A locus-restricted and tumor-specific CTLs in tumor-infiltrating lymphocytes of patients with non-small cell lung cancer.

Seki N., Hoshino T., Kikuchi M., Hayashi A., Itoh K.

Cell. Immunol. 175:101-110(1997).

DOI PubMed

Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.

Bodmer W.F.

Proc. Natl. Acad. Sci. U.S.A. 90:2842-2845(1993).

DOI PubMed PMC

Expression of cytoskeletal-associated protein tyrosine phosphatase PTPH1 mRNA in human hepatocellular carcinoma.

Yachi A.

J. Gastroenterol. 29:727-732(1994).

DOI PubMed

Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model.

Semple T.U., Quinn L.A., Woods L.K., Moore G.E.

Cancer Res. 38:1345-1355(1978).

PubMed

Web Resources

tcpaportal.org