NP-2Homo sapiens (Human)Cancer cell line
Also known as: NB3911, Case 4, NP 2
🤖 AI SummaryBased on 3 publications
Quick Overview
Human glioma cell line with MMAC1/PTEN mutations and p16/p53 alterations.
Detailed Summary
The NP-2 cell line is a human glioma cell line derived from malignant gliomas. It exhibits significant genetic alterations, including mutations in the MMAC1 (PTEN) gene, which is a critical tumor suppressor involved in cell cycle regulation and apoptosis. These mutations include rare 3-bp deletions and a nonsense mutation, suggesting functional impairment of the MMAC1 protein. Additionally, NP-2 shows alterations in the p16 and p53 tumor suppressor genes, with homozygous deletions and missense mutations, indicating potential roles in glioma progression. The cell line has been used to study the relationship between genetic mutations and tumor development, providing insights into the molecular mechanisms of glioma carcinogenesis.
Research Applications
Analysis of MMAC1/PTEN gene mutations in gliomasStudy of p16 and p53 gene alterations in glioma progressionInvestigation of genetic abnormalities in glioma cell lines
Key Characteristics
Mutations in MMAC1 (PTEN) geneHomozygous deletions in p16 and p53 genesMissense and nonsense mutations in tumor suppressor genes
Generated on 6/18/2025
Basic Information
| Database ID | CVCL_1R15 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Brain, left frontal lobe[UBERON:UBERON_0002811] |
Donor Information
| Age | 63 |
|---|---|
| Age Category | Adult |
| Sex | Male |
Disease Information
| Disease | Glioblastoma |
|---|---|
| Lineage | CNS/Brain |
| Subtype | Glioblastoma |
| OncoTree Code | GB |
DepMap Information
| Source Type | JCRB |
|---|---|
| Source ID | ACH-001608_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.Met237Ile (c.711G>T) | Unspecified | - | from parent cell line T98G |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
12
D13S317
10,12
D16S539
9,10,11
D18S51
13,15
D21S11
29
D3S1358
15,16
D5S818
11
D7S820
11
D8S1179
14,17
FGA
23
Penta D
12
Penta E
11
TH01
6
TPOX
8,11
vWA
16
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Frequent and variable abnormalities in p14 tumor suppressor gene in glioma cell lines.
Washiyama K., Kumanishi T., Uematsu Y., Itakura T.
Brain Tumor Pathol. 25:9-17(2008).
Rare-type mutations of MMAC1 tumor suppressor gene in human glioma cell lines and their tumors of origin.
Washiyama K., Kumanishi T.
Jpn. J. Cancer Res. 90:934-941(1999).
A comparative study of glioma cell lines for p16, p15, p53 and p21 gene alterations.
Washiyama K., Kumanishi T.
Jpn. J. Cancer Res. 87:900-907(1996).
The NP-2 cell system for analyses of the coreceptor usage of human and simian immunodeficiency viruses.
Hoshino H.
Kitakanto Med. J. 62:1-14(2012).