NP-3Homo sapiens (Human)Cancer cell line
Also known as: NB3972, Case 2, NP 3
🤖 AI SummaryBased on 3 publications
Quick Overview
Human glioma cell line with MMAC1 and p16 mutations
Detailed Summary
The NP-3 cell line is a human glioma cell line derived from malignant gliomas. It exhibits mutations in the MMAC1 (PTEN) gene, including rare 3-bp deletions and a nonsense mutation, which are associated with tumor suppressor dysfunction. Additionally, NP-3 shows alterations in the p16 gene, including homozygous deletions and missense mutations, contributing to cell cycle regulation disruption. These genetic changes are linked to glioma progression and provide a model for studying tumor suppressor gene inactivation in brain cancers. The cell line has been used to investigate the relationship between genetic mutations and tumor development in gliomas, offering insights into potential therapeutic targets.
Research Applications
Study of MMAC1 (PTEN) gene mutations in gliomasInvestigation of p16 gene alterations in tumor suppressionAnalysis of genetic mutations in glioma cell lines
Key Characteristics
Mutations in MMAC1 (PTEN) geneHomozygous deletions in p16 geneMissense mutations in p16 gene
Generated on 6/18/2025
Basic Information
| Database ID | CVCL_1R16 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Brain, left frontal lobe[UBERON:UBERON_0002811] |
Donor Information
| Age | 64 |
|---|---|
| Age Category | Adult |
| Sex | Male |
Disease Information
| Disease | Glioblastoma |
|---|---|
| Lineage | CNS/Brain |
| Subtype | Glioblastoma |
| OncoTree Code | GB |
DepMap Information
| Source Type | JCRB |
|---|---|
| Source ID | ACH-001609_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | PTEN | p.Met199del (c.592_594ATG[1]) (c.595_597delATG) | Unspecified | - | PubMed=10551321 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X,Y
CSF1PO
10,11
D13S317
13
D16S539
11
D18S51
23
D21S11
32.2
D3S1358
15,16
D5S818
9,10
D7S820
10
D8S1179
14
FGA
20,22
Penta D
13
Penta E
16
TH01
7,9
TPOX
9,11
vWA
17,19
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Frequent and variable abnormalities in p14 tumor suppressor gene in glioma cell lines.
Washiyama K., Kumanishi T., Uematsu Y., Itakura T.
Brain Tumor Pathol. 25:9-17(2008).
Rare-type mutations of MMAC1 tumor suppressor gene in human glioma cell lines and their tumors of origin.
Washiyama K., Kumanishi T.
Jpn. J. Cancer Res. 90:934-941(1999).