EJMHomo sapiens (Human)Cancer cell line

🤖 AI SummaryBased on 14 publications

Quick Overview

EJM is a human multiple myeloma cell line derived from a peritoneal effusion, characterized by IgG2 plasma cell morphology and ...

Detailed Summary

EJM is a human multiple myeloma cell line established from a peritoneal effusion of a patient with extramedullary myeloma. It exhibits plasma cell morphology with abundant rough endoplasmic reticulum and grows in liquid culture with a doubling time of 72 hours. The cell line is positive for CD9, CD20, CD32, CD38, and CD5, and expresses IgG2. EJM has been used in studies investigating the biology of multiple myeloma, including the characterization of MUM1/IRF4-IgH fusion events and MYC translocations. It is also utilized in research on the role of the NF-κB pathway in myeloma progression and in evaluating the efficacy of therapeutic agents targeting myeloma-specific genetic alterations.

Research Applications

Characterization of myeloma-specific genetic alterationsInvestigation of MUM1/IRF4-IgH fusion eventsStudy of MYC translocations in myelomaAnalysis of NF-κB pathway activation in myeloma progressionEvaluation of therapeutic agents targeting myeloma-specific genetic alterations

Key Characteristics

IgG2 plasma cell morphologyCD9, CD20, CD32, CD38, CD5 positivityGrowth in liquid culture with a doubling time of 72 hoursExpression of IgG2Utilized in studies of multiple myeloma biology and therapeutic strategies

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2030
Species	Homo sapiens (Human)
Tissue Source	Ascites[UBERON:UBERON_0007795]

Donor Information

Age	58
Age Category	Adult
Sex	Female

Disease Information

Disease	Multiple myeloma
Lineage	Lymphoid
Subtype	Plasma Cell Myeloma
OncoTree Code	PCM

DepMap Information

Source Type	DSMZ
Source ID	ACH-000821_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Lys132Asn (c.396G>C)	Heterozygous	-	Unknown, Unknown
MutationSimple	KRAS	p.Gly12Ala (c.35G>C)	Unspecified	-	PubMed=21173094

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,12

D13S317

D16S539

D18S51

15,17

D19S433

14,15

D21S11

31.2

D2S1338

18,25

D3S1358

15,17

D5S818

9,11

D7S820

D8S1179

FGA

20,22

Penta D

Penta E

TH01

9.3

TPOX

8,11

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

Evaluating the efficacy of multiple myeloma cell lines as models for patient tumors via transcriptomic correlation analysis.

Sirota M., Wiita A.P.

Leukemia 34:2754-2765(2020).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOI PubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Characterization of MYC translocations in multiple myeloma cell lines.

Dib A., Gabrea A., Glebov O.K., Bergsagel P.L., Kuehl W.M.

J. Natl. Cancer Inst. Monogr. 39:25-31(2008).

DOI PubMed PMC

Promiscuous mutations activate the noncanonical NF-kappaB pathway in multiple myeloma.

Stewart A.K., Carpten J.D., Bergsagel P.L.

Cancer Cell 12:131-144(2007).

DOI PubMed PMC

Activated fibroblast growth factor receptor 3 is an oncogene that contributes to tumor progression in multiple myeloma.

Kuehl W.M., Bergsagel P.L.

Blood 97:729-736(2001).

DOI PubMed

Malignant hematopoietic cell lines: in vitro models for the study of multiple myeloma and plasma cell leukemia.

Drexler H.G., Matsuo Y.

Leuk. Res. 24:681-703(2000).

DOI PubMed

Detection of MUM1/IRF4-IgH fusion in multiple myeloma.";

Shimizu S., Taniwaki M., Ueda R.

Leukemia 13:1812-1816(1999).

DOI PubMed

Fluorescence in situ hybridization analysis shows the frequent occurrence of 14q32.3 rearrangements with involvement of immunoglobulin switch regions in myeloma cell lines.

Lokhorst H.M., Clevers H.C., Bast B.J.E.G.

Cancer Genet. Cytogenet. 109:99-107(1999).

DOI PubMed

Characterization of new IgG lambda myeloma plasma cell line (EJM): a further tool in the investigation of the biology of multiple myeloma.

Hamilton M.S., Ball J., Bromidge E., Lowe J.L., Franklin I.M.

Br. J. Haematol. 75:378-384(1990).

DOI PubMed

Mutations of the p53 gene in human myeloma cell lines.";

Theillet C., Klein B.

Oncogene 7:1015-1018(1992).

PubMed

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI

Multiple myeloma cell lines.";

Jernberg-Wiklund H., Nilsson K.

(In book chapter) Human cell culture. Vol. 3. Cancer cell lines part 3; Masters J.R.W., Palsson B.O. (eds.); pp.81-155; Kluwer Academic Publishers; New York; USA (2000).

DOI

Web Resources

keatslab.org