ENHomo sapiens (Human)Cancer cell line
🤖 AI SummaryBased on 5 publications
Quick Overview
Human endometrial carcinoma cell line with TP53 mutation
Detailed Summary
The EN cell line is a human endometrial carcinoma cell line established from an invasive endometrioid adenocarcinoma of the uterine corpus in a 50-year-old patient. It exhibits rapid growth in culture with a doubling time of 24.4 hours and high migration activity. The cells show a tendency to pile up without contact inhibition. Subcutaneous transplantation of EN cells into nude mice forms solid tumors diagnosed as adenocarcinoma, with no metastasis observed. EN cells produce tissue polypeptide antigen and exhibit high telomerase activity. Genetic analysis reveals a TP53 mutation at codon 273, resulting in a stop codon at codon 308. The cell line also shows expression of estrogen receptor β but not α or progesterone receptors.
Research Applications
Cancer biology researchDrug sensitivity testingTumor formation studiesGenetic mutation analysis
Key Characteristics
Rapid in vitro growthHigh telomerase activityTP53 mutation at codon 273Expression of estrogen receptor βNo metastasis in xenograft models
Generated on 6/18/2025
Basic Information
| Database ID | CVCL_2034 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Endometrium[UBERON:UBERON_0001295] |
Donor Information
| Age | 50 |
|---|---|
| Age Category | Adult |
| Sex | Female |
| Race | asian |
Disease Information
| Disease | Endometrial carcinoma |
|---|---|
| Lineage | Uterus |
| Subtype | Endometrial Carcinoma |
| OncoTree Code | UCEC |
DepMap Information
| Source Type | DSMZ |
|---|---|
| Source ID | ACH-000978_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.Arg273Glnfs (c.817_818insA) | Unspecified | - | PubMed=12581894 |
| MutationSimple | STAG2 | p.Leu264Pro (c.791T>C) | Homozygous | - | Unknown, Unknown, PubMed=25010205 |
| MutationSimple | PTEN | p.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA) | Heterozygous | - | Unknown, Unknown, PubMed=25230021 |
| MutationSimple | PTEN | p.Leu186Valfs (c.556C>CG) | Heterozygous | - | PubMed=20944090 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
12,14
D13S317
8,11
D16S539
11,12
D18S51
14,15
D19S433
16,17
D21S11
30,34.2
D2S1338
20,25
D3S1358
16,18
D5S818
12,13
D7S820
11
D8S1179
15,17
FGA
18,19,20
Penta D
10,13
Penta E
11,17
TH01
6,9
TPOX
8,11
vWA
14,17
Gene Expression Profile
Gene expression levels and statistical distribution
Loading cohorts...
Full DepMap dataset with combined data across cell lines
Loading gene expression data...
Publications
Pan-cancer proteomic map of 949 human cell lines.";
Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Cancer Cell 40:835-849.e8(2022).
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Sellers W.R.
Nature 569:503-508(2019).
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
Cancer Res. 79:1263-1273(2019).
Characterization of human cancer cell lines by reverse-phase protein arrays.
Liang H.
Cancer Cell 31:225-239(2017).
A landscape of pharmacogenomic interactions in cancer.";
Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
Cell 166:740-754(2016).
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Loewer M., Sahin U., Castle J.C.
Genome Med. 7:118.1-118.7(2015).
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
Nature 483:603-607(2012).
PTEN deficiency in endometrioid endometrial adenocarcinomas predicts sensitivity to PARP inhibitors.
Lord C.J., Ashworth A., Reis-Filho J.S.
Sci. Transl. Med. 2:53ra75.1-53ra75.8(2010).