FKH-1Homo sapiens (Human)Cancer cell line
Also known as: FKH1
🤖 AI SummaryBased on 4 publications
Quick Overview
Human myeloid leukemia cell line with t(6;9) translocation and dek-can fusion.
Detailed Summary
The FKH-1 cell line is a human myeloid leukemia cell line established from a patient with t(6;9)(p23;q34) translocation, which results in the expression of a dek-can chimaeric transcript. This cell line is notable for its ability to proliferate and differentiate into various myeloid lineages, including macrophages, basophils, eosinophils, and neutrophils, when cultured with G-CSF. The FKH-1 cells exhibit a doubling time of approximately 54 hours and have been used in research to study the role of the dek-can fusion protein in leukaemogenesis. Additionally, the cell line has been utilized in investigations of microsatellite instability and mutations in genes such as BAX, TGFβRI, hMSH3, hMSH6, and DRP. The FKH-1 cell line is also mentioned in the context of myeloid neoplasms with eosinophilia, where it was found to have ZMYM2-FLT3 and DIAPH1-PDGFRB gene fusions, although these findings are not directly linked to the FKH-1 cell line itself. The cell line has been maintained for over 57 months without EB virus contamination, and it shows a consistent karyotype of 46.XY.t(6:9)(p23:34).
Research Applications
Study of t(6;9)(p23;q34) translocation and dek-can chimaeric transcriptInvestigation of myeloid differentiation and proliferationAnalysis of microsatellite instability and mutations in BAX, TGFβRI, hMSH3, hMSH6, and DRP genesResearch on myeloid neoplasms with eosinophilia
Key Characteristics
Expresses dek-can chimaeric transcriptCapable of multilineage myeloid differentiationMaintained for over 57 months without EB virus contaminationConsistent karyotype of 46.XY.t(6:9)(p23:34)
Generated on 6/18/2025
Basic Information
| Database ID | CVCL_2041 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Peripheral blood[UBERON:UBERON_0000178] |
Donor Information
| Age | 61 |
|---|---|
| Age Category | Adult |
| Sex | Male |
Disease Information
| Disease | Acute myeloid leukemia |
|---|---|
| Lineage | Myeloid |
| Subtype | Acute Myeloid Leukemia |
| OncoTree Code | AML |
DepMap Information
| Source Type | DSMZ |
|---|---|
| Source ID | ACH-002651_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| Gene fusion | DEK | DEK-NUP214, DEK-CAN | - | - | PubMed=9753053 |
| Gene fusion | ABL1 | ETV6-ABL1 | - | In frame | PubMed=28751768 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X,Y
CSF1PO
11,12
D13S317
8,10
D16S539
10,11
D18S51
15,17
D19S433
14,16
D21S11
29,32
D2S1338
18,22
D3S1358
16,17
D5S818
12,13
D7S820
12
D8S1179
10,15
FGA
19,22
Penta D
10,13
Penta E
12,16
TH01
8,9
TPOX
8,11
vWA
17,18
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Cytogenetically cryptic ZMYM2-FLT3 and DIAPH1-PDGFRB gene fusions in myeloid neoplasms with eosinophilia.
Tapper W.J., Reiter A., Cross N.C.P.
Leukemia 31:2271-2273(2017).
Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Leuk. Res. 25:275-278(2001).
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Leuk. Res. 24:255-262(2000).
Establishment of a novel human myeloid leukaemia cell line (FKH-1) with t(6;9)(p23;q34) and the expression of dek-can chimaeric transcript.
Eguchi M.
Br. J. Haematol. 102:1249-1256(1998).
The leukemia-lymphoma cell line factsbook.";
Drexler H.G.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).