HNT-34Homo sapiens (Human)Cancer cell line

Also known as: HNT34

🤖 AI SummaryBased on 11 publications

Quick Overview

Human myeloid leukemia cell line with t(3;3)(q21;q26) and t(9;22)(q34;q11) translocations.

Detailed Summary

HNT-34 is a human myeloid leukemia cell line established from a patient with AML transformed from MDS with 3q21q26 syndrome. It exhibits t(3;3)(q21;q26) and t(9;22)(q34;q11) translocations, along with the expression of EV11 gene, P210, and P190 BCR/ABL chimaeric transcripts. The cell line is characterized by undifferentiated blasts with specific surface markers and is used in studies related to myeloid malignancies and leukemogenesis mechanisms. It has been utilized in research on JAK2 V617F mutations and other genetic alterations in hematological cancers.

Research Applications

Myeloid malignanciesLeukemogenesis mechanismsJAK2 V617F mutation studiesBCR/ABL chimeric transcripts analysis

Key Characteristics

t(3;3)(q21;q26) and t(9;22)(q34;q11) translocationsExpression of EV11 gene, P210, and P190 BCR/ABL chimaeric transcriptsUndifferentiated blasts with specific surface markers

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2071
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age	47
Age Category	Adult
Sex	Female

Disease Information

Disease	Acute myeloid leukemia
Lineage	Myeloid
Subtype	Acute Myeloid Leukemia
OncoTree Code	AML

DepMap Information

Source Type	Academic lab
Source ID	ACH-000299_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
Gene fusion	ABL1	BCR-ABL1, BCR-ABL	-	BCR exon 1 fused to ABL1 exon 2	PubMed=10576511, PubMed=10071072, PubMed=8751477

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,13

D13S317

10,11

D16S539

9,12

D18S51

13,16

D19S433

D21S11

28,32.2

D2S1338

18,24

D3S1358

D5S818

D7S820

8,11

D8S1179

13,14

FGA

20,26

Penta D

Penta E

11,14

TH01

6,9

TPOX

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOI PubMed

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

Leukemia 20:471-476(2006).

DOI PubMed

Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Leuk. Res. 25:275-278(2001).

DOI PubMed

Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Leuk. Res. 24:255-262(2000).

DOI PubMed

p53 alterations in human leukemia-lymphoma cell lines: in vitro artifact or prerequisite for cell immortalization?

Uphoff C.C.

Leukemia 14:198-206(2000).

DOI PubMed

ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.";

Uphoff C.C., Habig S., Fombonne S., Matsuo Y., Drexler H.G.

Leuk. Res. 23:1055-1060(1999).

DOI PubMed

Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.

Drexler H.G., MacLeod R.A.F., Uphoff C.C.

Leuk. Res. 23:207-215(1999).

DOI PubMed

Establishment of a novel human myeloid leukaemia cell line (HNT-34) with t(3;3)(q21;q26), t(9;22)(q34;q11) and the expression of EVI1 gene, P210 and P190 BCR/ABL chimaeric transcripts from a patient with AML after MDS with 3q21q26 syndrome.

Morishita K.

Br. J. Haematol. 98:399-407(1997).

DOI PubMed

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI