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KCL-22Homo sapiens (Human)Cancer cell line

Also known as: KCL22-s, KCL22-S, KCL22S, Kcl22, KCL22

🤖 AI SummaryBased on 11 publications

Quick Overview

KCL-22 is a Ph1+ CML cell line with double Ph1 chromosomes and additional abnormalities.

Detailed Summary

KCL-22 is a Ph1-positive chronic myeloid leukemia (CML) cell line established from a patient in blast crisis. It exhibits double Ph1 chromosomes and additional chromosomal abnormalities, including trisomy 8 and isochromosome 17. The cell line shows characteristics of immature myeloid cells, with positive staining for periodic acid-Schiff and acid phosphatase. It lacks lymphoid markers and Epstein-Barr virus (EBV) nuclear antigen, indicating its origin from CML blasts. KCL-22 is used in research on CML pathogenesis, drug resistance mechanisms, and molecular characterization of Ph1-positive leukemias. It has been utilized in studies investigating the role of BCR-ABL fusion genes, chromosomal translocations, and imatinib resistance mechanisms.

Research Applications

Chronic myeloid leukemia researchPh1 chromosome studiesBCR-ABL fusion gene analysisImatinib resistance mechanismsChromosomal translocation studies

Key Characteristics

Ph1-positiveDouble Ph1 chromosomesAdditional chromosomal abnormalitiesImmature myeloid cell characteristicsLack of lymphoid markers

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2091
Species	Homo sapiens (Human)
Tissue Source	Pleural effusion[UBERON:UBERON_0000175]

Donor Information

Age	32
Age Category	Adult
Sex	Female
Subtype Features	BCR-ABL1 positive

Disease Information

Disease	Chronic myeloid leukemia
Lineage	Myeloid
Subtype	Chronic Myeloid Leukemia, BCR-ABL1+
OncoTree Code	CMLBCRABL1

DepMap Information

Source Type	DSMZ
Source ID	ACH-000983_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Pro301Glnfs*44 (c.902delC)	Heterozygous	-	PubMed=33087752
MutationSimple	MSH6	p.Phe1088Leufs*5 (c.3261dupC) (3261_3262insC)	Heterozygous	-	from parent cell line KCL-22
MutationSimple	MSH6	p.Phe1088Serfs*2 (c.3261delC)	Homozygous	-	from parent cell line Jurkat
Gene fusion	ABL1	BCR-ABL1, BCR-ABL	-	BCR exon 1 fused to ABL1 exon 2	PubMed=10576511, PubMed=10071072, PubMed=8751477

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X

CSF1PO

11,12

D13S317

8

D16S539

11,12

D18S51

17

D19S433

13,14

D21S11

29,34.2

D2S1338

19,20

D3S1358

15,18

D5S818

10,11

D7S820

11,12

D8S1179

10,11,13,14

FGA

22,26

Penta D

9,13

Penta E

17,19

TH01

7,9

TPOX

8

vWA

14

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

The LL-100 panel: 100 cell lines for blood cancer studies.";

MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.

Sci. Rep. 9:8218-8218(2019).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

Identification of annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells.

Ruoppolo M., Salvatore F.

Proteomics 13:2414-2418(2013).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

Gaining insights into the Bcr-Abl activity-independent mechanisms of resistance to imatinib mesylate in KCL22 cells: a comparative proteomic approach.

Pane F., Ruoppolo M., Salvatore F.

Biochim. Biophys. Acta 1804:1974-1987(2010).

Cytogenetic characterisation and proteomic profiling of the Imatinib-resistant cell line KCL22-R.

Loncarevic I.F.

Int. J. Oncol. 31:121-128(2007).

ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.";

Uphoff C.C., Habig S., Fombonne S., Matsuo Y., Drexler H.G.

Leuk. Res. 23:1055-1060(1999).

Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.

Drexler H.G., MacLeod R.A.F., Uphoff C.C.

Leuk. Res. 23:207-215(1999).

Frameshift mutations of the hMSH6 gene in human leukemia cell lines.

Hirai H.

Jpn. J. Cancer Res. 89:33-39(1998).

Two new human myeloid cell lines derived from acute promyelocytic leukemia and chronic myelocytic leukemia.

Miyoshi I.

Gann 74:319-322(1983).

Establishment of a Ph1 chromosome-positive cell line from chronic myelogenous leukemia in blast crisis.

Kubonishi I., Miyoshi I.

Int. J. Cell Cloning 1:105-117(1983).

Ph positive CML cell lines.";

Keating A.

Baillieres Clin. Haematol. 1:1021-1029(1987).

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).