KYO-1Homo sapiens (Human)Cancer cell line

Also known as: Kyoto 1, KYO1

🤖 AI SummaryBased on 7 publications

Quick Overview

KYO-1 is a Ph' chromosome-positive cell line derived from CML in blast crisis, used for studying differentiation and proliferat...

Detailed Summary

KYO-1 is a Ph' chromosome-positive cell line established from the peripheral blood of a patient with chronic myelogenous leukemia (CML) in blastic crisis. This cell line exhibits the unique capacity to differentiate along the erythroid and monocytic lineages for the first several months, although this capability gradually diminishes with repeated passages. KYO-1 cells are considered undifferentiated myeloid cells and provide a valuable model for studying the differentiation and proliferation of pluripotent stem cells from CML in blastic crisis. The cell line has been maintained in continuous culture for over three years with a doubling time of approximately 22 hours. KYO-1 retains colony-forming ability in agar cultures, with a significant proportion of colonies containing α-NBE positive cells, indicating some degree of differentiation potential.

Research Applications

Study of differentiation and proliferation of pluripotent stem cellsInvestigation of erythroid and monocytic differentiationAnalysis of Ph' chromosome-positive CML cell lines

Key Characteristics

Ph' chromosome-positiveUndifferentiated myeloid cellsSpontaneous differentiation along erythroid and monocytic lineages for initial passagesContinuous culture for over three yearsColony-forming ability in agar cultures

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2095
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age	22
Age Category	Adult
Sex	Male
Race	asian
Subtype Features	BCR-ABL1 positive

Disease Information

Disease	Chronic myeloid leukemia
Lineage	Myeloid
Subtype	Chronic Myeloid Leukemia, BCR-ABL1+
OncoTree Code	CMLBCRABL1

DepMap Information

Source Type	DSMZ
Source ID	ACH-000604_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg248Gln (c.743G>A)	Unspecified	Somatic mutation acquired during proliferation	PubMed=20575032
Gene fusion	ABL1	BCR-ABL1, BCR-ABL	-	BCR exon 1 fused to ABL1 exon 2	PubMed=10576511, PubMed=10071072, PubMed=8751477

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,14

D13S317

D16S539

D18S51

D19S433

14,16

D21S11

D2S1338

D3S1358

16,18

D5S818

10,13

D7S820

D8S1179

12,14

FGA

18,25

Penta D

10,11

Penta E

11,15,17

TH01

6,7

TPOX

9,11

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

DOI PubMed PMC

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.";

Uphoff C.C., Habig S., Fombonne S., Matsuo Y., Drexler H.G.

Leuk. Res. 23:1055-1060(1999).

DOI PubMed

Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.

Drexler H.G., MacLeod R.A.F., Uphoff C.C.

Leuk. Res. 23:207-215(1999).

DOI PubMed

A novel Ph1 chromosome positive cell line established from a patient with chronic myelogenous leukemia in blastic crisis.

Ohkubo T., Kamamoto T., Kita K., Hiraoka A., Yoshida Y., Uchino H.

Leuk. Res. 9:921-926(1985).

DOI PubMed

Ph positive CML cell lines.";

Keating A.

Baillieres Clin. Haematol. 1:1021-1029(1987).

DOI PubMed

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI